Abstract: A method for oxidizing an alkyl, including a) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the ?-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, and b) contacting the aqueous solution from a) with a water-immiscible organic solvent.
Type:
Grant
Filed:
October 7, 2020
Date of Patent:
August 23, 2022
Assignee:
Evonik Operations GmbH
Inventors:
Thomas Haas, Oliver Thum, Jan Christoph Pfeffer, Philip Engel, Christian Gehring, Markus Poetter
Abstract: The present disclosure relates to the use of beta-glucosidase to enhance the production efficiency of desired steviol glycosides, such as rebaudioside M (reb M).
Type:
Grant
Filed:
December 23, 2019
Date of Patent:
August 16, 2022
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Michael Batten, Oliver Yu
Abstract: Provided herein are improved compositions and methods for enzyme replacement therapy using modified human cystathionine beta synthase (CBS) in the treatment of homocystinuria and related diseases and disorders.
Type:
Grant
Filed:
June 24, 2020
Date of Patent:
August 2, 2022
Assignee:
THE REGENTS OF THE UNIVERSITY OF COLORADO
Inventors:
Jan P. Kraus, Tomas Majtan, Erez Bublil
Abstract: A method of demethylizing an opioid to a nor-opioid is provided. The method comprises contacting an opioid with at least one enzyme. Contacting the opioid with the at least one enzyme converts the opioid to a nor-opioid. A method of converting a nor-opioid to a nal-opioid is provided. The method comprises contacting a nor-opioid with at least one enzyme. Contacting the nor-opioid with the at least one enzyme converts the nor-opioid to a nal-opioid.
Type:
Grant
Filed:
July 1, 2020
Date of Patent:
July 26, 2022
Assignee:
Antheia, Inc.
Inventors:
Christina D. Smolke, Catherine Thodey, Isis Trenchard
Abstract: The present invention relates to alpha-amylase variants comprising substitutions at positions corresponding to positions 268 and 293 of SEQ ID NO: 1, in particular substitutions selected from the group consisting of: 268G+293Y; 268G+293F; 268G+293W; 268G+293H; 268G+293A; 268G+293Q; 268A+293Y; 268A+293F; 268A+293W; 268A+293H; 268A+293A; 268A+293Q; 268P+293Y; 268P+293F; 268P+293W; 268P+293H; 268P+293A; 268P+293Q; 268S+293Y; 268S+293F; 268S+293W; 268S+293H; 268S+293A; 268S+293Q; 268T+293Y; 268T+293F; 268T+293W; 268T+293H; 268T+293A; 268T+293Q; 268V+293Y; 268V+293F; 268V+293W; 268V+293H; 268V+293A; 268V+293Q; 268I+293Y; 268I+293F; 268I+293W; 268I+293H; 268I+293A; 268I+293Q; 268L+293Y; 268L+293F; 268L+293W; 268L+293H; 268L+293A; 268L+293Q; 268M+293Y; 268M+293F; 268M+293W; 268M+293H; 268M+293A; 268M+293Q; and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a
Type:
Grant
Filed:
December 7, 2018
Date of Patent:
July 12, 2022
Assignee:
NOVOZYMES A/S
Inventors:
Carsten Andersen, Lars Giger, Johanne Mørch Jensen, Louis Patrick Lessard
Abstract: To provide a method for industrially producing a sugar carboxylic acid from an oxidized starch decomposition product or oxidized transfer reaction product having a degree of polymerization of 2 or more in a high yield, using a catalase formulation which quickly decomposes hydrogen peroxide produced as a by-product in the oxidation.
Abstract: The use of a cytochrome P-450 enzyme comprising SEQ ID NO: 3, or a variant enzyme having at least 70% identity thereto and having CYP-450 activity, for the hydroxylation of an organic compound.
Type:
Grant
Filed:
November 15, 2017
Date of Patent:
July 12, 2022
Assignee:
HYPHA DISCOVERY LIMITED
Inventors:
Jonathan Charles Paul Steele, Antonio De Riso, Headley St Edward Williams, Richard Kerry Phipps, Stephen Keith Wrigley, Kinga Linda Nytko, Vincent Poon, Sebastian Schulz, John Maxim Ward
Abstract: The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.
Type:
Grant
Filed:
June 4, 2019
Date of Patent:
June 28, 2022
Assignee:
Teknologian Tutkimuskeskus VTT Oy
Inventors:
Kaisa Marjamaa, Anu Koivula, Nina Aro, Tiina Pakula
Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Abstract: The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.
Type:
Grant
Filed:
February 10, 2020
Date of Patent:
June 21, 2022
Assignee:
The Regents of the University of California
Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
Type:
Grant
Filed:
May 15, 2018
Date of Patent:
June 21, 2022
Assignee:
Promega Corporation
Inventors:
Andrew S. Dixon, Lance P. Encell, Thomas Machleidt, Marie Schwinn, Keith Wood, Monika Wood, Kris Zimmerman
Abstract: A saccharification reaction mixture wherein the reaction mixture can saccharify at least one of cellulose and hemicellulose and contains at least one of cellulose and hemicellulose, a saccharification enzyme, silica or a silica-containing substance, and at least one compound (A) selected from the group including a polyhydric alcohol compound represented by the following formula (1) or a derivative thereof and an acetylene glycol represented by formula (2) or an alkylene oxide adduct thereof. The symbols in the chemical formulas are defined in the specification.
Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.
Type:
Grant
Filed:
June 1, 2020
Date of Patent:
June 14, 2022
Assignee:
Genomatica, Inc.
Inventors:
Andreas W. Schirmer, Noah Helman, Haibo Wang, Zhihao Hu, Vikranth Arlagadda, Alma Itzel Ramos-Solis, Elizabeth Clarke
Abstract: The invention provides a mutant of a parent heterotrimeric G protein alpha (G?) subunit, which mutant (i) lacks at least one helix of the helical domain of the parent G? subunit; (ii) is capable of binding to a GPCR in the absence of a heterotrimeric G protein beta (G?) subunit and a heterotrimeric G protein gamma (G?) subunit; and (iii) has an amino acid sequence that contains one or more mutations compared to the amino acid sequence of the parent heterotrimeric G? subunit, which mutations are selected from a deletion, a substitution and an insertion.
Type:
Grant
Filed:
March 31, 2020
Date of Patent:
May 24, 2022
Assignee:
Heptares Therapeutics Limited
Inventors:
Byron Carpenter, Andrew Leslie, Rony Nehmé, Christopher Gordon Tate, Antony Warne
Abstract: A method of increasing terpenoid production in a host cell that produces one or more terpenoids, comprising: a) providing a host cell that produces one or more terpenoids, said host cell comprising a vector comprising a polynucleotide sequence encoding a terpene synthase enzyme; b) modifying the vector to: i. introduce an inducible promoter operably linked to the polynucleotide sequence encoding the terpene synthase enzyme; and ii. introduce a polynucleotide sequence encoding a ribosomal binding site (RBS) that increases translation initiation rate of the terpenoid compared to a wild type ribosomal binding site; c) determining the dosage of an inducer capable of inducing the inducible promoter; d) culturing the host cell in a culture medium in the presence of the inducer at the dosage determined from step c); and e) isolating the terpenoid from the culture medium.
Type:
Grant
Filed:
July 17, 2020
Date of Patent:
May 17, 2022
Assignee:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Abstract: The present disclosure relates to a method for preparing a transfructosylated steviol glycoside using Arthrobacter-derived microorganisms, a culture thereof, a supernatant of the culture, an extract of the culture, and a lysate of the microorganisms.
Type:
Grant
Filed:
October 25, 2018
Date of Patent:
May 17, 2022
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Tae Joo Yang, In Sung Kang, Min Hoe Kim, Sunghee Park, Sun Chu, Seong Bo Kim, Young Mi Lee, Young Su Lee, Eun Jung Choi
Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.
Type:
Grant
Filed:
July 21, 2020
Date of Patent:
May 10, 2022
Assignee:
ERBER AKTIENGSELLSCHAFT
Inventors:
Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
Abstract: The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae exhibiting decreased level of BCY1 protein activity and capable of anaerobic fermentation of xylose into ethanol without the need for cell growth. Also provided are methods of using such genetically engineered yeast strains for improved anaerobic xylose fermentation in the yeast for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.