Patents Examined by Iqbal H Chowdhury
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Patent number: 11976310Abstract: The present disclosure relates to a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps: (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1). The disclosure also relates to enzymes for catalyzing the corresponding enzymatic reactions and recombinant microorganisms which express the enzymes for catalyzing the corresponding enzymatic reactions.Type: GrantFiled: February 16, 2021Date of Patent: May 7, 2024Inventors: Arren Bar-Even, Hai He, Philippe Marliere
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Patent number: 11976311Abstract: The present invention provides for a method of fermenting or saccharifying a biomass comprising: (a) (i) contacting a biomass comprising a polysaccharide, and an ionic liquid (IL) to form a first solution, or (ii) providing the first solution comprising the biomass and the IL, (b) contacting the first solution and carbon dioxide such that the first solution results in a lower pH, (c) introducing (i) an enzyme capable of enzymatically to breakdown at least one bond in the polysaccharide or a breakdown product of the polysaccharide, and/or (ii) a microorganism that capable of producing the enzyme and/or fermenting the polysaccharide or a breakdown product of the polysaccharide, such that the polysaccharide is at least partially broken down and the first solution is transformed into a second solution.Type: GrantFiled: November 16, 2020Date of Patent: May 7, 2024Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Jian Sun, Jian Shi, Blake A. Simmons, Seema Singh
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Patent number: 11970723Abstract: The present disclosure discloses a strain producing D-allulose 3-epimerase and application thereof, and belongs to the technical field of bioengineering. The present disclosure provides a method for improving the expression of D-allulose 3-epimerase by screening promoters and optimizing RBS thereof. The recombinant Bacillus subtilis constructed using thevectors pP43NMK-hag and pP43NMK-hag-RBS4 provided by the present disclosure improves the enzyme activity of a target gene D-allulose 3-epimerase, and theenzyme activities in shake flasks upon transformation are 1.30 times and 1.69 times that of an original vector. The present disclosure further provides a non-antibiotic resistance vector and a non-antibiotic resistance recombinant B. subtilis strain. Using the non-antibiotic resistance strain B. subtilis 1A751-dal-/pP43NMK-hag-RBS4-dpe-dal provided by the present disclosure, the highest fermentation enzyme activity in a shake flask is 24.72 U/mL, and the enzyme activity in a fermenter is 714.8 U/mL.Type: GrantFiled: March 22, 2023Date of Patent: April 30, 2024Assignee: JIANGNAN UNIVERSITYInventors: Tao Zhang, Mengying Hu, Bo Jiang, Mengli Li
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Patent number: 11964316Abstract: A method for reducing odor in remediation of pesticide chemical contaminated soil comprises preparing odor control sustained-release particles and evenly covering on the surface of the pesticide chemical contaminated soil according to a certain dosage; preparing odor covering base materials and mixed bacterial solution, and preparing the odor covering soil by mixing the odor covering base materials and mixed bacterial solution in proportion, and then covering the odor covering soil on the surface of the pesticide chemical contaminated soil; and inserting heating rods into the pesticide chemical contaminated soil, and heating the surface layer of the pesticide chemical contaminated soil to 30-40° C.Type: GrantFiled: December 1, 2022Date of Patent: April 23, 2024Assignee: Nanjing Institute of Environmental Sciences, MEEInventors: Shengtian Zhang, Mei Li, Lu Yang, Jinzhong Wan, Qun Li, Tingting Fan, Yan Zhou, Yuanchao Zhao, Xiang Wang
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Patent number: 11965201Abstract: The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways.Type: GrantFiled: March 16, 2021Date of Patent: April 23, 2024Assignee: LanzaTech NZ, Inc.Inventors: Kaspar Valgepea, Michael Koepke, James Bruce Yarnton Haycock Behrendorff, Esteban Marcellin, Lars K. Nielsen, Renato de S. P. Lemgruber
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Patent number: 11959117Abstract: The present invention includes novel systems, methods, and compositions for the enzymatic/chemical production of pseudouridine (?) and its variants, such as N1-methyl-pseudouridine-5?-triphosphate (m1?TP).Type: GrantFiled: June 6, 2023Date of Patent: April 16, 2024Assignee: NATURE'S TOOLBOX, INC.Inventors: Alexander Koglin, Michael Humbert, Matthias Strieker, Grant Eliason
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Patent number: 11957722Abstract: The present invention discloses an anti-aging composition, which includes: (a) isolated lactic acid bacterial strains or a fermented product thereof; and (b) an excipient, a diluent, or a carrier; wherein the isolated lactic acid bacterial strains include: Bifidobacterium bifidum VDD088 strains, Bifidobacterium breve Bv-889 strains, and Bifidobacterium longum BLI-02 strains. The present invention further provides a method for preventing aging by administering the foregoing anti-aging composition to a subject in need thereof.Type: GrantFiled: March 7, 2022Date of Patent: April 16, 2024Assignee: GLAC BIOTECH CO., LTDInventors: Hsieh-Hsun Ho, Yi-Wei Kuo, Wen-Yang Lin, Jia-Hung Lin, Yen-Yu Huang, Chi-Huei Lin, Shin-Yu Tsai
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Patent number: 11952596Abstract: Application of glutamate dehydrogenase GdhA of Peptostreptococcus asaccharolyticus in increasing the yield of poly-?-glutamic acid from Bacillus licheniformis. The glutamate dehydrogenase GdhA of the Bacillus licheniformis WX-02 per se is replaced with the glutamate dehydrogenase derived from the Peptostreptococcus asaccharolyticus by means of homologous recombination, which significantly increases the level of synthesizing the poly-?-glutamic acid for the Bacillus licheniformis, and the yield of the obtained poly-?-glutamic acid from strains is increased at least by more than 20% compared with control strains.Type: GrantFiled: September 30, 2020Date of Patent: April 9, 2024Assignee: Hubei UniversityInventors: Shouwen Chen, Fan Yang, Dongbo Cai, Yaozhong Chen, Qing Zhang, Xin Ma, Jiangang Chen
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Patent number: 11932672Abstract: Some embodiments relate to a method for producing a product of interest with a microbial host using an auto-replicative extra-chromosomal nucleic acid molecule comprising a first nucleic acid sequence whose genetic activity confers an advantage to the host, optionally wherein the genetic activity of said first nucleic acid molecule is controlled.Type: GrantFiled: December 19, 2018Date of Patent: March 19, 2024Assignees: Syngulon S.A., Université Libre de BruxellesInventors: Philippe Gabant, Mohamed El Bakkoury, Laurence Van Melderen
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Patent number: 11921118Abstract: Provided herein are methods for labeling the proteomes of cells, as well as methods for labeling proteins or populations of proteins produced by cells. In some embodiments, the methods comprise introducing variant aminoacyl-tRNA synthetases and noncanonical amino acids into cells. Also provided herein are polynucleotides encoding variant aminoacyl-tRNA synthetases that recognize noncanonical amino acids. The methods and compositions provided herein are useful for, among other things, identifying target cells and identifying biomarkers of interest.Type: GrantFiled: November 5, 2020Date of Patent: March 5, 2024Assignees: The Board of Trustees of the Leland Stanford Junior University, The United States Government as represented by the Department of Veterans AffairsInventors: Andrew Yang, Anton Wyss-Coray, Kyle Brewer
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Patent number: 11920120Abstract: Provided herein are isolation processes and the associated hardware to allow fluid streams to be isolated from a sterilized system (e.g., a sterile process vessel) that contains a sterile process. The isolation processes described herein allow for continuous removal of fluid streams (e.g., waste streams, liquid containing recombinant therapeutic proteins) from a sterilized system (e.g., a biological manufacturing system), which provides for less manual manipulation of the sterilized system and a decreased risk of contaminating the sterilized system.Type: GrantFiled: November 24, 2021Date of Patent: March 5, 2024Assignee: Genzyme CorporationInventor: Robert Snow
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Patent number: 11913034Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.Type: GrantFiled: July 21, 2021Date of Patent: February 27, 2024Assignee: DSM IP ASSETS B.V.Inventors: Johannes Gustaaf Ernst Van Leeuwen, Priscilla Zwartjens, Viktor Marius Boer
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Patent number: 11913046Abstract: Microbial cells genetically engineered with a heterologous nucleic acid sequence that increases export of 2? fucosyllactose are disclosed. Methods of increasing export of 2? fucosyllactose from a microbial cell and for identifying a heterologous nucleic acid sequence that increases export of 2? fucosyllactose from a microbial cell are also disclosed.Type: GrantFiled: April 23, 2018Date of Patent: February 27, 2024Assignee: INBIOSE N.V.Inventors: Kerry Hollands, Lori Anne Maggio-Hall, Steven Cary Rothman
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Patent number: 11912742Abstract: The present disclosure includes a construct comprising a leupeptin biosynthesis operon, the operon comprising leupA, leupB, leupC, and leupD genes operably linked to a promoter. Also included are cells comprising the construct, and methods for production of leupeptin peptide.Type: GrantFiled: June 30, 2021Date of Patent: February 27, 2024Assignee: YALE UNIVERSITYInventors: Jason Crawford, Jhe-Hao Li, Joonseok Oh
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Patent number: 11905513Abstract: Methods of genetically modifying microorganisms using advanced genome editing are disclosed. Methods of modifying the genome of these microorganisms for point mutations, deletions, and DNA insertions are also disclosed. Further, inhibiting expression of genes without manipulating the genome of the microorganism is disclosed. In some cases, the microorganism can be a methylotroph, e.g., a methanotroph.Type: GrantFiled: October 11, 2018Date of Patent: February 20, 2024Assignee: MBP TITAN, LLCInventors: Xuezhi Li, James Kealey, Kevin Lee Dietzel
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Patent number: 11905540Abstract: A method for producing L-lysine by fermentation, comprising modifying a gene for coding an NCBI reference sequence NP_601029.1 and/or NP_599350.1 on a Corynebacterium bacterial chromosome to enable the activity and/or expression quantity of NP_601029.1 and/or NP_599350.1 to be reduced; replacing a promoter of one or more genes on the Corynebacterium bacterial chromosome with a EP5 promoter, and fermenting bacteria obtained by modification to produce L-lysine. Also provided are methods and applications derived from the method, and bacteria and promoter that can used in the methods and the applications.Type: GrantFiled: December 21, 2021Date of Patent: February 20, 2024Assignee: NINGXIA EPPEN BIOTECH CO., LTDInventors: Gang Meng, Aiying Wei, Fengyong Ma, Huiping Jia, Jiyin Ma
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Patent number: 11896034Abstract: The present invention relates animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. The invention also relates to polypeptides having lysozyme activity, polynucleotides encoding the polypeptides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: March 25, 2021Date of Patent: February 13, 2024Assignee: Novozymes A/SInventors: Mikkel Klausen, Kirk Matthew Schnorr, Lars Kobberoee Skov, Soeren Nymand-Grarup, Marianne Thorup Cohn, Peter Bjarke Olsen, Ming Li, Ye Liu
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Patent number: 11898172Abstract: The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.Type: GrantFiled: March 29, 2018Date of Patent: February 13, 2024Assignee: Genomatica, Inc.Inventors: Kevin Hoff, Cara Ann Tracewell, Kui Chan, Michael Kuchinskas, Harish Nagarajan
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Patent number: 11891591Abstract: The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions.Type: GrantFiled: November 3, 2021Date of Patent: February 6, 2024Assignee: NOVOZYMES A/SInventors: Kim Borch, Carsten Hørslev Hansen, Jesper Vind, Marco Malten, Svend Gunnar Kaasgaard
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Patent number: 11884947Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.Type: GrantFiled: February 22, 2019Date of Patent: January 30, 2024Assignee: ShanghaiTech UniversityInventors: Jia Chen, Li Yang, Xingxu Huang, Bei Yang, Xiao Wang, Jianan Li