Patents Examined by Iqbal H Chowdhury
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Patent number: 11753615Abstract: The present disclosure belongs to the field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a genetically engineered strain of Saccharomyces cerevisiae, method for constructing the same, and its use for brewing, the genetically engineered strain of Saccharomyces cerevisiae heterogeneously overexpresses an acetaldehyde dehydrogenase gene ALD6, an acetyl-CoA synthase gene ACS1 and an alcohol acyltransferase gene AeAT9. The Saccharomyces cerevisiae strain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.Type: GrantFiled: August 25, 2021Date of Patent: September 12, 2023Assignee: Tianjin University of Science and TechnologyInventors: Yefu Chen, Wenqi Shi, Guo Zhang, Ruirui Li, Yanfang Chen, Huan Wang, Chunhong Sun, Dongguang Xiao, Xiaole Wu, Xuewu Guo
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Patent number: 11753646Abstract: Provided are a tagatose-bisphosphate aldolase variant having tagatose conversion activity, and a method of preparing tagatose using the same.Type: GrantFiled: September 27, 2019Date of Patent: September 12, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Young Mi Lee, Eul-Soo Park, Il Hyang Park, Sun Mi Shin, Sung Jae Yang, Ran Young Yoon, Eun Jung Choi, Seong Bo Kim, Seung Won Park
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Patent number: 11746351Abstract: A genetically engineered microorganism for the production of a cannabinoid biosynthetic pathway product is described. The genetically engineered microorganism comprises at least one nucleic acid molecule encoding at least one cannabinoid biosynthetic pathway enzyme. The disclosure also relates to methods for producing a cannabinoid biosynthetic pathway product using a genetically engineered microorganism.Type: GrantFiled: April 29, 2019Date of Patent: September 5, 2023Assignee: Algae-C Inc.Inventors: William Mather Almon Carscallen, Isabel Desgagné-Penix, Jean-Francois Lemay
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Patent number: 11746130Abstract: The present disclosure relates to a novel protein variant having an activity of exporting 5?-inosine monophosphate, a microorganism comprising the protein variant, and a method for preparing 5?-inosine monophosphate using the microorganism.Type: GrantFiled: June 6, 2022Date of Patent: September 5, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Jin Ah Rho, Byoung Hoon Yoon, So-jung Park, Min Ji Baek, Ji Hye Lee
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Patent number: 11739337Abstract: Disclosed is a synthetic gene cluster for producing gadusol derivatives, expression vectors and host cells containing the same, methods of producing gadusol derivatives, and compositions thereof. In an example, the synthetic gene cluster includes a valA nucleotide sequence capable of expressing ValA protein; a nucleotide sequence capable of expressing methyltransferase/oxidoreductase (MT-Ox) protein; a mysC nucleotide sequence capable of expressing a MysC protein; and a mysD nucleotide sequence capable of expressing a MysD protein. In this way, gadusol derivatives can be produced in amounts sufficient for use in a variety of applications.Type: GrantFiled: September 4, 2020Date of Patent: August 29, 2023Assignee: OREGON STATE UNIVERSITYInventors: Taifo Mahmud, Andrew Robert Osborn
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Patent number: 11725224Abstract: Disclosed are methods, systems, components, and compositions for cell-free synthesis of proteins and glycoproteins. The methods, systems, components, and compositions may be utilized for incorporating non-standard amino acids (nsAAs) into cell-free synthesized proteins and glycosylating or otherwise modifying the cell-free synthesized proteins in vitro. The nsAAs of the cell-free synthesized protein may be modified via glycosylation or other modification.Type: GrantFiled: April 16, 2019Date of Patent: August 15, 2023Assignee: Northwestern UniversityInventors: Michael Christopher Jewett, Jessica Carol Stark, Jasmine Hershewe
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Patent number: 11725197Abstract: The present invention relates to a variant of a parent lipase, wherein said variant has at least 60% but less than 100% sequence identity with SEQ ID NO: 2; has lipase activity; has substitutions corresponding to G91A and N92D in the lid region located from position G82 to position 1100 of SEQ ID NO: 2; has one or more additional substitutions in the lid region; and has at least mutations in positions corresponding to position T231 and position N233 of SEQ ID NO: 2. The present invention also relates to compositions, methods for cleaning a surface using a variant or a composition of the invention; polynucleotides encoding the variants; nucleic acid constructs, vectors, host cells comprising the polynucleotides; methods of using the variants; and methods for obtaining the lipase variant.Type: GrantFiled: December 3, 2018Date of Patent: August 15, 2023Assignee: Novozymes A/SInventors: Allan Svendsen, Sune Fang Christensen, Lars Lehman Hylling Christensen, Vikram Kjoller Bhatia, Jesper Vind, Vibeke Skovsgaard Nielsen
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Patent number: 11718649Abstract: The present invention is based on the fusion of FimH, CsgA and PapG adhesins (type 1 Fimbriae, curli and P, respectively) to generate biomolecules that can be used as vaccines against UTIs. Briefly, the invention is based on the design of a fusion template gene fimH-csgA-papG-fimH-csgA and defined as fcpfc to generate the different combinations of monomeric [FimH, CsgA and PapG], dimeric [FimH-CsgA (FC), CsgA-PapG (CP) and PapG-FimH (PF)] and trimeric (FimH-PapG-CsgA (FCP), PapG-CsgA-FimH (PCF) and CsgA-FimH-PapG (CFP)] proteins. Bioactivity was determined by the antibodies present in UP sera and urine, the quantification in the release of cytokines and adhesion inhibition assays.Type: GrantFiled: October 11, 2018Date of Patent: August 8, 2023Inventors: Juan Xicohtencatl Cortes, Victor Manuel Luna Pineda
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Patent number: 11708567Abstract: The present invention provides several non-naturally occurring sulfotransferase enzymes that have been engineered to react with aryl sulfate compounds as sulfo group donors, instead of the natural substrate 3?-phosphoadenosine 5?-phosphosulfate (PAPS), and with heparosan-based polysaccharides, particularly heparan sulfate, as sulfo group acceptors. Each of the engineered sulfotransferase enzymes have a biological activity characterized by the position within the heparosan-based polysaccharide that receives the sulfo group, including glucosaminyl N-sulfotransferase activity, hexuronyl 2-O sulfotransferase activity, glucosaminyl 6-O sulfotransferase activity, or glucosaminyl 3-O sulfotransferase activity. Methods of using the engineered sulfotransferases to produce sulfated heparosan-based polysaccharides, including polysaccharides having anticoagulant activity, are also provided.Type: GrantFiled: August 24, 2022Date of Patent: July 25, 2023Assignee: OPTIMVIA, LLCInventors: Tarsis Gesteira Ferreira, Daniel H. Lajiness
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Patent number: 11697826Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.Type: GrantFiled: May 24, 2019Date of Patent: July 11, 2023Assignee: Inscripta, Inc.Inventors: Ryan T. Gill, Andrew Garst, Tanya Elizabeth Warnecke Lipscomb
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Patent number: 11692204Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.Type: GrantFiled: December 3, 2018Date of Patent: July 4, 2023Assignee: JIANGNAN UNIVERSITYInventors: Jian Jin, Yun Chen, Huazhong Li, Songtao Zhou, Zuoying Duan, Xiaohai Gong
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Patent number: 11685937Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.Type: GrantFiled: May 11, 2020Date of Patent: June 27, 2023Assignee: Danisco US Inc.Inventors: Marguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
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Patent number: 11667885Abstract: The present description is related to the field of cultivating algae. It introduces a method of cultivating algae by depleting the culture of an inorganic nutrient and exposing the alga to high intensity light to obtain algal cell mass having enriched lipid content and reduced chlorophyll content.Type: GrantFiled: November 4, 2015Date of Patent: June 6, 2023Assignees: NESTE OYJ, SUOMEN YMPÄRISTÖKESKUS SYKEInventors: Reijo Tanner, Jukka Seppälä, Timo Tamminen, Kristian Spilling, Pasi Ylöstalo, Pauliina Uronen
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Patent number: 11667901Abstract: Provided are chondrosulfatase and a use thereof, belonging to the technical field of biological engineering. Chondrosulfatase is screened and identified from the natural world, the maximum similarity between its amino acid sequence and that of the chondrosulfatase reported by NCBI being 85%; then the expression in Escherichia coli and Bacillus subtilis is optimized, achieving the high-efficiency biosynthesis of chondrosulfatase having high enzymatic activity, the maximum enzyme activity being 11976.5 U/L; furthermore, the entire process and post-processing are simpler. The invention has potentially broad value in application in the preparation of products containing low molecular-weight chondroitin sulfate in the fields of medicine, cosmetics, and biology, lays the foundation for efficient fermentation of a microbial system to produce a chondrosulfatase having high enzyme activity, and is suitable for industrialized production applications.Type: GrantFiled: September 18, 2019Date of Patent: June 6, 2023Assignee: NANJING HANXIN PHARMACEUTICAL INCInventors: Haoning Zhang, Song Chen, Chuangen Tang, Jing Wang
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Patent number: 11667935Abstract: The invention relates to a process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell, capable of converting sugar, glycerol and acetic acid, wherein the carbon source comprises sugar and acetic acid, comprising the following steps: a) Inoculating a optionally diluted carbon source with the cell; b) optionally fermenting the reactor in batch mode; c) adding carbon source comprising glycerol and optionally sugar gradually to the reactor; d) after sufficient fermentation time, isolation of fermentation product from the reactor, e) optionally keeping the remaining fraction after isolation of step d) as spent broth; and f) optionally using the spent broth in step a) to dilute the carbon source.Type: GrantFiled: November 12, 2020Date of Patent: June 6, 2023Assignee: DSM IP ASSETS B.V.Inventors: Hans Marinus Charles Johannes De Bruijn, Wilhelmus Theodorus Antonius Maria De Laat, Paul Klaassen
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Patent number: 11653679Abstract: Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2, reb M2, and reb I are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.Type: GrantFiled: August 21, 2015Date of Patent: May 23, 2023Assignee: PURECIRCLE SDN BHDInventors: Avetik Markosyan, Indra Prakash, Cyrille Jarrin, Aurelien Badie, Robert Ter Halle, Cynthia Curran, Daniel Auriol
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Patent number: 11655476Abstract: A modular and hierarchical DNA assembly platform for synthetic biology is described. This enabling technology, termed MIDAS (for Modular Idempotent DNA Assembly System), can precisely assemble multiple DNA fragments in a single reaction using a standardised assembly design. It can be used to build genes from libraries of sequence-verified, reusable parts and to assemble multiple genes in a single vector. We describe the design and use of MIDAS, and its application in the reconstruction of the metabolic pathway for production of paspaline, a key intermediate in the biosynthesis of a range of indole diterpenes—a class of economically important secondary metabolites produced by several species of filamentous fungi.Type: GrantFiled: September 28, 2018Date of Patent: May 23, 2023Assignee: Victoria Link LlimitedInventors: Emily Jane Parker, Matthew Joseph Nicholson, Craig John Van Dolleweerd
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Patent number: 11655465Abstract: The disclosure provides novel topoisomerases which exhibit weakened ability to form a covalent bond to the 5?-(C/T)CCTT-3? recognition site in the presence of increased NaCl concentrations relative to wild-type, together with novel methods for synthesizing DNA in the 3? to 5? direction using the novel topoisomerase; and other compounds, compositions, methods and devices comprising or utilizing topoisomerases which exhibit reduced covalent bond formation to the 5?-(C/T)CCTT-3? recognition site in the presence of increased NaCl concentrations, relative to wild-type.Type: GrantFiled: May 4, 2020Date of Patent: May 23, 2023Assignee: IRIDIA, INC.Inventors: Paul F. Predki, Stefen Boehme
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Patent number: 11649450Abstract: The disclosure provides methods of making a protein having a desired non-standard amino acid incorporated at its N-terminus in a cell and methods of screening for an amino acyl tRNA synthetase variant that preferentially selects a non-standard amino acid against its standard amino acid counterpart or undesired non-standard amino acids for incorporation into a protein in a cell.Type: GrantFiled: June 29, 2018Date of Patent: May 16, 2023Assignee: President and Fellows of Harvard CollegeInventors: Aditya Mohan Kunjapur, George M. Church
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Patent number: 11649512Abstract: The invention relates to the use of a polysaccharide having at least four saccharide units, such as stachyose, as a glass-forming agent for the freeze-drying of a reaction mixture comprising an enzyme. In particular, the enzyme is a polymerase useful in a nucleic acid amplification reaction such as a Polymerase Chain Reaction. Compositions comprising such polysaccharides as well as methods for preparing them, kits containing them and methods for using them form further aspects of the invention.Type: GrantFiled: March 9, 2020Date of Patent: May 16, 2023Assignee: FLUOROGENICS LTD.Inventors: Martin Lee, Diane Lee, Mark Laverick