Abstract: This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

Abstract: A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).

Abstract: The present invention relates to a safflower plant or part thereof involving the molecular stacking of safflower events IND-1ØØØ3-4 x IND-1ØØ15-7, wherein the plant produces and accumulates chymosin in seed under agricultural conditions. A plant seed involving the molecular stacking of safflower events IND-1ØØØ3-4 x IND-1ØØ15-7. A consumer product produced from the seed, defined as chymosin, and additionally as ground grain, flour, flakes, oil, biodiesel, biogas, or another biomaterial. Also, the present invention include a recombinant DNA molecule involved in the molecular stacking of safflower events IND-1ØØØ3-4 x IND-1ØØ15-7. A DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of the DNA molecule involved in the molecular stacking of safflower events IND-1ØØØ3-4 x IND-1ØØ15-7, or its complement which is useful in a DNA amplification method to produce a diagnostic amplicon for the event IND-1ØØØ3-4 and IND-1ØØ15-7, or each of them separately IND-1ØØØ3-4 and IND-1ØØ15-7.

Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).

Abstract: Provided is a food processor and an attachment for a food processor. The attachment having a substantially S-shaped vertical rotational member having an upper end and a lower end, the lower end of the rotational member configured for secure engagement to a base motor. There is also a first blade assembly attached to a lower portion of the rotational member, the first blade assembly having at least one upward-angled blade, and a second blade assembly attached to an upper portion of the rotational member, the second blade assembly having at least one downward-angled blade. The first blade assembly and second blade assembly are disposed along the rotational member such that the first blade assembly and second blade assembly offset one another along the height and width of the housing.

Abstract: This invention relates to compositions and methods for modifying Growth Regulating Factor (GRF) family transcription factors in plants to produce plants having improved phenotypic characteristics including increased growth. The invention further relates to plants produced using the methods and compositions of the invention.

Abstract: The present invention relates to a safflower plant or part thereof involving the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7, wherein the plant produces and accumulates chymosin in seed under agricultural conditions. A plant seed involving the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7. A consumer product produced from the seed, defined as chymosin, and additionally as ground grain, flour, flakes, oil, biodiesel, biogas, or another biomaterial. Also, the present invention include a recombinant DNA molecule involved in the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7. A DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of the DNA molecule involved in the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7, or its complement which is useful in a DNA amplification method to produce a diagnostic amplicon for the event IND-1ØØØ3-4 and IND-1ØØ15-7, or each of them separately IND-1ØØØ3-4 and IND-1ØØ15-7.

Abstract: A gene PpHSP21 with black spot disease resistance is isolated from Pyrus pyrifolia. A nucleotide sequence of the gene PpHSP21 is shown in SEQ ID NO. 1. An amino acid sequence of an encoded protein of the PpHSP21 gene is shown in SEQ ID NO. 2. By constructing the plant overexpression vector and silencing vector of the gene PpHSP21, the gene PpHSP21 is introduced into the plant by the Agrobacterium-mediated genetic transformation method, so that the gene PpHSP21 is able to be overexpressed in the plants, thereby significantly improving black spot disease resistance in plants. The discovery and identification of the gene PpHSP21 provide new genetic resources for stress resistance molecular design and breeding in plants, and to provide new genetic resources for the implementation of green agriculture. The development and utilization of the genetic resources is conducive to reducing agricultural production costs and achieving environmental friendliness.

Abstract: Described herein are methods for producing a hot forged material in air using a Ni-based superalloy for the die. Also described herein are methods for producing a hot forged material, by placing on a lower die a material for hot forging, of which part or all of the surface is coated with a glass-based lubricant, and pressing the material for hot forging with the lower die and an upper die, to form a hot forged material, wherein one or both of the lower die and the upper die are made of a Ni-based superalloy, and wherein the surface of the die in contact with the material for hot forging is coated with a glass-based lubricant containing SiO2 as a main component, in which the total content of alkali metal oxides is 0 to 10.0% in mass %.

Abstract: The invention relates to a device (1) for securing a tool (2), in particular a bending tool, comprising a receiving. space (4) for receiving an engaging part (5) of the tool and means (9) arranged in or close to the receiving space for locating and/or communicating with the tool. The device is further provided with a first protective layer (11) at least partially separating the locating and/or communication means from the receiving space. The tool can comprise a locating and/or communication element (14) which co-acts with the locating and/or communication means and which is closed off from the surrounding area by a second protective layer (15). The invention further relates to a method for manufacturing such a securing device.

Abstract: A mandrel for forming a stent with a tapered profile and one or more anti-migration features includes a first stent shaping segment having a first diameter, a second stent shaping segment having a second diameter less than the first diameter and a tapered segment disposed therebetween. A third stent shaping segment is releasably securable to the second stent shaping segment and has a third diameter greater than the second diameter. One or more movable pins are outwardly extendable from corresponding apertures formed within the tapered segment. An actuation element is engagable with the first stent shaping segment and includes a tapered surface configured to engage the one or more movable pins and support the one or more movable pins extended from the corresponding apertures.

Abstract: A press brake is provided with a hydraulic cylinder configured to move an upper table and a lower table relative to each other in a vertical direction, and a control unit configured to control a hydraulic circuit of the hydraulic cylinder, in which the control unit manages a predictor of an occurrence of an abnormality in the hydraulic circuit including a first pressure control valve configured to control a back pressure of hydraulic oil on a first port side of the hydraulic cylinder.

Abstract: A punch and a pad sandwiching a top sheet portion, bending blades for bend-forming the vertical wall portions, and stoppers facing the bending blades are provided. The punch is supported by a first cushion component. The bending blades each have an upper die component and a lower die component disposed facing each other in the press direction with an interval equal to a set compression amount in the range of 2% or more and 6% or less of the heights of the vertical wall portions and a second cushion component interposed between the upper die component and the lower die component, maintaining the interval, and contractible in the press direction. The cushion pressure of the second cushion component is lower than the cushion pressure of the first cushion component and has such cushion pressure that the second cushion component does not contract during the bend-forming of the vertical wall portions.

Abstract: A machine for shredding portions of bituminous material in the solid-state into granules in the solid-state. The machine comprises a pressing member provided with a piston which is axially mobile inside a perforated tubular body along an axis and is provided on the head thereof with an internal concave portion having a shape substantially complementary to a projecting part present in an axial end of the perforated tubular body. On the head of the piston through-openings are made, which extend radially along a direction approximately transversal to the axis and are in communication with the internal space of the concave portion.

Abstract: The present invention has as its object to provide a method for manufacturing a shaped part able to suppress the occurrence of shaping defects when manufacturing a tubular shaped part. The above object is achieved by providing a method for manufacturing a shaped part comprising a first bending process of press-forming a metal sheet to obtain a curved intermediate part curved in a longitudinal direction by a curvature ?, a U-process of press-forming the curved intermediate part into an approximately U-section to obtain a first curved U-section intermediate part provided with a bottom part having the curvature ?, and an O-process of press-forming the first curved U-section intermediate part to form a closed cross-section to obtain a tubular shaped part provided with a bottom part having the curvature ?.

Abstract: A bender configured to bend a workpiece in a bending operation and its method of use are provided. The bender includes a frame, a bending shoe assembly rotatably mounted on the frame, a control system and a driver in communication therewith, and a springback assembly in communication with the control system and configured to provide information to the control system regarding information on a bend affected to the workpiece. The bending shoe assembly includes a bending shoe into which the workpiece can be seated, and a gripping member mounted on the bending shoe and configured to grip the workpiece during the bending operation. The driver provides rotational force to the bending shoe assembly to rotate the bending shoe assembly relative to the frame.

Abstract: A tight space pilot, tight space pilot assembly, and related method includes a tight space pilot that can be installed into a die without fasteners, fastener apertures, or added blocks for mounting. The tight space pilot assembly includes ejectors that are retained by the pilot and a retaining ring that holds the tight space pilot in place in a die member.

Abstract: in a method for producing a solder deposit in a metal sheet, a depression is made in a topside of the metal sheet through deep drawing, thereby causing material to protrude on a bottom side of the metal sheet. The metal sheet is then subjected to a material forming process to produce a collar such that the collar projects in relation to the topside. The collar is then at least partially pressed in a direction of the depression to reduce a cross-sectional area of a mouth of the depression, and the protruding material on the bottom side is completely pushed back so that the bottom side in a region of the depression is in one plane with neighboring regions of the bottom side.

Abstract: The invention relates to a method for producing a connecting element comprising at least two ball-like sections, wherein two ball-like sections are directly adjacent to each other, or wherein two ball-like sections are connected to each other by means of a connecting section, wherein the rolling of a single-piece blank, which is cylindrical at least in sections, between at least two rolling tools and the shaping of the at least two ball-like sections and optionally of the connecting section during the rolling are provided.

Abstract: A tool, such as a conduit bender, that includes a gear assembly for providing a mechanical advantage when bending a workpiece conduit. The gear assembly comprises a pinion gear rotatably coupled to a handle, the handle including a pin that can be selectively engaged with the pinion gear. The conduit bender also includes a shoe with teeth that protrude radially inward from a curved outer portion. When the conduit bender is in use, the teeth of the pinion gear engage with the teeth of the shoe.