Patents Examined by Nghi V Nguyen
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Patent number: 11141510Abstract: A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.Type: GrantFiled: March 22, 2019Date of Patent: October 12, 2021Assignee: Revotek Co., Ltd.Inventors: Yujian James Kang, Xiao Zuo
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Patent number: 11111510Abstract: The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO, CO2 or H2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.Type: GrantFiled: December 20, 2019Date of Patent: September 7, 2021Assignees: LanzaTech New Zealand Limited, DBT-IOC Centre for Advanced Bio-Energy ResearchInventors: Sean Dennis Simpson, Sebastian Michal Bernasek, Deepak K Tuli
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Patent number: 11098278Abstract: The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.Type: GrantFiled: October 7, 2019Date of Patent: August 24, 2021Assignee: OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Xiupin Wang, Peiwu Li, Huali Xie, Xuefang Wang, Qi Zhang, Xiaoxia Ding, Wen Zhang, Liangxiao Zhang
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Patent number: 11077441Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.Type: GrantFiled: June 3, 2016Date of Patent: August 3, 2021Assignee: Sphere Fluidics LimitedInventors: Frank F. Craig, Marian Rehak, David Holmes, Clive A. Smith, Xin Liu, Giuseppe Benazzi, Xin Li, Vinayaka Pawate, Robert Frank Marchington, Richard Francis Day, Michael Stuart Hazell
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Patent number: 11078454Abstract: A bioreactor and method of forming complex three-dimensional tissue constructs in a single culture chamber. The bioreactor and methods may be used to form multi-phasic tissue constructs having tissue formed from multiple cell types in a single culture chamber. The bioreactor includes at least one translation mechanism to facilitate translation of one or more tissue constructs without direct user intervention, thereby providing a closed, sterile environment for complex tissue fabrication. The bioreactor may be used as a stand-alone device or as part of a large-scale system including many bioreactors. The large-scale system may include a perfusion system to monitor and regulate the tissue culture environment.Type: GrantFiled: November 21, 2018Date of Patent: August 3, 2021Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Lisa M. Larkin, Ellen M. Arruda, Michael J. Smietana, Pablo Moncada-Larrotiz
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Patent number: 11051535Abstract: The present disclosure relates to a method of isolating a yeast strain that is able to degrade L-asparagine under non-inducing conditions comprising repeated cycles of adaptive evolution and mutagenesis followed by strain selection. Also included are yeast strains obtained by the method, and methods and uses thereof for reducing asparagine, and thus acrylamide, during food preparation and processing.Type: GrantFiled: July 6, 2016Date of Patent: July 6, 2021Assignee: Renaissance BioScience Corp.Inventors: Zachari J. Turgeon, Jessica Marie Swanson, Matthew S. Dahabieh, John I. Husnik
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Patent number: 11046931Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.Type: GrantFiled: April 1, 2019Date of Patent: June 29, 2021Assignee: Coming IncorporatedInventors: Stephen Joseph Caracci, David Henry, Jessica Jo Kelley, Mark Alan Lewis, Yue Zhou
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Patent number: 11046947Abstract: Methods and devices are provided for simultaneous irradiation of a target, such as mammalian cells and/or nanostructures. The target is simultaneously exposed to optical irradiation and oscillating magnetic field radiation. The simultaneous optical irradiation and oscillating magnetic field radiation occur within an incubator-actuator device. The incubator-actuator device includes a sample chamber, a magnetic field generating coil, and, a light-emitting diode (LED) placement cage.Type: GrantFiled: September 29, 2016Date of Patent: June 29, 2021Assignee: Southeast Missouri State UniversityInventors: Santaneel Ghosh, Somesree GhoshMitra, Jian Peng
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Patent number: 11041180Abstract: Provided a complex bacteria for improving the corn steeping effects and usage thereof. The complex bacteria comprises strains capable of producing protease, cellulase and hemicellulase. Using the complex bacteria to steep corn, effects of reducing the addition amount of SO2, shortening the steeping time and improving the starch yield are achieved.Type: GrantFiled: March 19, 2020Date of Patent: June 22, 2021Assignee: COFCO (Jilin) Bio-Chemical Technology Co., Ltd.Inventors: Yi Tong, Yi Li, Jin Tao, Bo Chen, Yuan Zhang
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Patent number: 11033586Abstract: The present invention provides a method of producing a cell sheet comprising a retinal pigment epithelial cell layer and a vascular forming cell layer composed of stem cells having vascular formation ability or blood vessel constituting cells, comprising a step of laminating the retinal pigment epithelial cell layer and the vascular forming cell layer, and a cell sheet obtained by said method. Furthermore, the present invention provides a cell sheet for transplantation, comprising a cell layer formed with retinal pigment epithelial cells obtained by inducing differentiation of stem cells or progenitor cells ex vivo, a basement membrane secreted from said cells, and a vascular forming cell layer composed of stem cells having vascular formation ability or blood vessel constituting cells.Type: GrantFiled: August 23, 2013Date of Patent: June 15, 2021Assignee: RIKENInventors: Masayo Takahashi, Hiroyuki Kamao
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Patent number: 11013844Abstract: A device and method can be used for clinical virus inactivation treatment to automatically, continuously and cyclically treat plasma of a patient in a closed system. The disclosed plasma treatment device may be used for an anti-viral agent such as, for example, methylene blue photochemical plasma virus inactivation. The plasma treatment device can perform real-time treatment to the plasma in the closed system on the basis of the principle of plasma exchange and can realize real-time back-transfusion during treatment.Type: GrantFiled: November 19, 2015Date of Patent: May 25, 2021Assignees: City of Hope, Shanghai Blood CenterInventors: Yuwen Huang, Qin Mo, Bo Zhang, Kaicheng Qian, Yongming Zhu, Shirong Wang, Xun Wang, Xiaofei Wu, Yao Jia, Lidong Liu
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Patent number: 11001800Abstract: A microalgae culturing method by using a paper-based culture apparatus, includes: 1) designing the shape of a culture apparatus as a rectangle with circles distributed in its middle; 2) spraying and printing wax on a piece of filter paper; 3) heating such that the wax penetrates through the filter paper to obtain a paper culture apparatus, where a surface of the filter paper on which the wax is sprayed and printed is called a surface A while the other surface thereof is called a surface B; and 4) dropping a first agar aqueous solution onto the circles on the surface B of the paper culture apparatus obtained in the step 3), and dropping a second agar aqueous solution added with microalgae onto the surface A of the paper culture apparatus obtained in the step 3).Type: GrantFiled: October 10, 2019Date of Patent: May 11, 2021Assignee: TIANJIN UNIVERSITYInventors: Guanyi Chen, Daohong Zhang, Beibei Yan, Zhan Hu, Yun Qi
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Patent number: 10988735Abstract: Described herein are tissues containing semiconductor nanomaterials. In some embodiments, the tissues include vascular cells, cardiomyocytes, and/or cardiac fibroblasts. The tissue may be scaffold-free. In some embodiments, the tissue includes an electrically conductive network. The tissue may exhibit synchronized electrical signal propagation within the tissue. In some embodiments, the tissue exhibits increased functional assembly of cardiac cells and/or increased cardiac specific functions compared to a cardiac tissue prepared using a conventional tissue culture method. Methods of preparing and using such tissues are also described herein.Type: GrantFiled: January 15, 2016Date of Patent: April 27, 2021Assignees: Clemson University Research Foundation, MUSC Foundation for Research Development, The University of ChicagoInventors: Ying Mei, Tan Yu, Dylan Richards, Donald R. Menick, Bozhi Tian
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Patent number: 10947577Abstract: A biological sterilization indicator device is provided. The device comprises a body, a plurality of test microorganisms, and an oxygen-modulated first fluorescent sensor. The body comprises a first layer attached to a second layer, forming at least one isolatable microchamber and at least one primary passageway that provides fluidic communication between ambience and the at least one microchamber. The microchamber has an isolated volume of about 0.5 microliters to about 9.5 microliters. The plurality of test microorganisms and the oxygen-modulated first fluorescent sensor are disposed in the microchamber. A method of using the device to determine the effectiveness of a sterilization process is also provided.Type: GrantFiled: February 11, 2013Date of Patent: March 16, 2021Assignee: 3M Innovative Properties CompanyInventors: Francois Ahimou, Sailaja Chandrapati, Kurt J. Halverson, Heather M. Webb, Michael G. Williams
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Patent number: 10947321Abstract: A method for producing Metal Organic Framework (MOF) having a framework that encapsulates a bio-molecule, the method comprising combining in a solution the bio-molecule and MOF precursors, wherein the bio-molecule promotes formation of the encapsulating framework. The method stems from a surprising effect that a bio-molecule can promote or trigger the formation of MOF when combined together in a solution with MOF precursors. That is, it has now been found that a bio-molecule can effectively act as a seed around which the framework forms, with the resulting framework encapsulating the bio-molecule.Type: GrantFiled: May 19, 2015Date of Patent: March 16, 2021Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Kang Liang, Raffaele Ricco, Cara Maxwell Doherty, Paolo Falcaro
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Patent number: 10920214Abstract: The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.Type: GrantFiled: May 31, 2013Date of Patent: February 16, 2021Assignee: NEWLEAF SYMBIOTICS, INC.Inventor: Gregg Bogosian
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Patent number: 10865376Abstract: A method detects the presence or absence of at least one first inhibition zone having a culture of biological particles on a culture medium in the presence of a chemical agent. The method of detection has a first step of seeding the culture medium with the biological particles, a second step in which the culture medium receives the chemical agent, a third step of incubation of the culture medium, and a fourth step of measurement of the first inhibition zone of the culture medium, which includes a first phase of taking an image of the first inhibition zone by ombroscopy.Type: GrantFiled: June 29, 2015Date of Patent: December 15, 2020Assignee: BIOMERIEUXInventors: Laurent Drazek, Frédéric Pinston
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Patent number: 10857264Abstract: Biomaterial having a multi-dimensional structure and comprising demineralized bone matrix dispersed within differentiated mesenchymal stem cells (MSCs) tissue, wherein MSCs are adipose tissue-derived stem cells. Method for producing this biomaterial comprising incubating MSCS in osteoblastic and/or chondrongenic medium in presence of demineralized bone matrix. Use of this biomaterial for alleviating or treating a bone or cartilage defect, supporting or correcting a congenital or acquired abnormality, supporting a bon or articular bone replacement following surgery or trauma, and/or supporting a musculoskeletal implant.Type: GrantFiled: July 7, 2017Date of Patent: December 8, 2020Assignees: Universite Catholique De Louvain, Cliniques Universitaires Saint-LucInventors: Denis Dufrane, Christian Delloye
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Patent number: 10822625Abstract: The present disclosure provides a method for domesticating Saccharomyces cerevisiae which is resistant to fermentation inhibitors, high temperature and high concentration of ethanol. The method comprises a primary domestication and a secondary domestication of the S. cerevisiae, wherein the primary domestication comprises the steps of replacing the fermented mash with fresh liquefied mash and continuing fermentation when the ethanol content in the fermented mash reaches a higher concentration (>12% (v/v)), the replacement is performed for at least 20 times, and the time interval of replacements is 20-30 hours; the secondary domestication comprises the steps of performing high-temperature domestication on the primary domesticated strain by a temperature gradient and replacing the fermented mash for multiple times, wherein the temperature is raised by 0.5° C.-2° C. every 18-24 times of replacement. The domesticated S.Type: GrantFiled: December 2, 2019Date of Patent: November 3, 2020Assignees: COFCO (JILIN) BIO-CHEMICAL TECHNOLOGY CO., LTD., COFCO BIO-ENERGY (ZHAODONG) CO., LTD., NUTRITION & HEALTH RESEARCH INSTITUTE, COFCO CORPORATIONInventors: Yi Tong, Yi Li, Junqi Zhang, Taibo He, Zexing Wang, Bo Hou, Hui Liu, Fan Li, Lida Wu
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Patent number: 10816557Abstract: Methods for testing a red blood cell (RBC) unit for release for transfusion into a subject are described. The methods allow management of the blood supply by assessing the viability of an RBC unit by its levels of dicarboxylic fatty acids (DFA). The methods include testing an RBC sample from an RBC unit for levels of DFA and discarding the RBC unit or releasing the RBC unit for transfusion based on the results.Type: GrantFiled: August 10, 2015Date of Patent: October 27, 2020Assignee: BloodworksInventor: James Charles Zimring