Abstract: The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.
Type:
Grant
Filed:
February 1, 2013
Date of Patent:
February 20, 2018
Assignee:
DANISCO US INC
Inventors:
Mark S. Gebert, Sang-Kyu Lee, Mariliz Johnson, Michael Ward
Abstract: Provided is a host cell comprising a rhlA gene or an ortholog thereof, under the control of a heterologous promoter and a rhlB gene or an ortholog thereof, under the control of a heterologous promoter. The host cell is capable of achieving a carbon yield of more than 0.18 Cmol rhamnolipid/Cmol substrate. Provided is also a method of producing rhamnolipids, employing such a host cell.
Type:
Grant
Filed:
September 21, 2012
Date of Patent:
January 2, 2018
Assignee:
Amlika Mercantile Private Limited
Inventors:
Lars Blank, Frank Rosenau, Susanne Wilhelm, Andreas Wittgens, Till Tiso
Abstract: The invention relates to a method of reacting one or more L-nucleotides with a first L-nucleic acid in the presence of a D-enzyme that adds the one or more L-nucleotides to the 3? end of the first L-nucleic acid.
Type:
Grant
Filed:
May 16, 2013
Date of Patent:
December 26, 2017
Assignee:
NOXXON Pharma AG
Inventors:
Andreas Pech, Ralf David, Florian Jarosch, Michael Jahnz, Sven Klussmann
Abstract: The present disclosure provides a sophorolipid composition that can be used for inducing protein expression in a fermentation host. The sophorolipid composition described herein can be prepared from a natural sophorolipid mixture. Acid treatment of the natural sophorolipid mixture results in a mixture of monoacetylated, deacetylated, and/or diacetylated sophorolipids. The chemically modified sophorolipid composition, or isolated components of the chemically modified sophorolipid composition, can be used as inducers for protein production in filamentous fungi.
Abstract: A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
Type:
Grant
Filed:
May 24, 2011
Date of Patent:
November 21, 2017
Assignee:
EVOLVA SA
Inventors:
Michael Patrik Katz, Thomas Durhuus, Hans Peter Smits, Jochen Förster
Abstract: Provided herein are compositions, reactions mixtures, mutant Ras proteins, kits, substrates, and systems for selecting a Ras antagonist, as well as methods of using the same.
Type:
Grant
Filed:
November 2, 2016
Date of Patent:
November 7, 2017
Assignee:
ARAXES PHARMA LLC
Inventors:
Matthew P. Patricelli, Ulf Peters, Liansheng Li, Pingda Ren, Yi Liu
Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Type:
Grant
Filed:
July 15, 2016
Date of Patent:
October 31, 2017
Assignee:
Codexis, Inc.
Inventors:
Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
Abstract: The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.
Type:
Grant
Filed:
July 20, 2012
Date of Patent:
October 24, 2017
Assignee:
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANIZATION
Inventors:
Colin Scott, John Oakeshott, Robyn Russell, Nigel French, Steven Kotsonis, Kaiyan Liu
Abstract: The present invention is directed towards a protein-polymer composition having a protein with a site-specifically incorporated unnatural amino acid initiator and a covalently attached polymer.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
October 17, 2017
Assignee:
Franklin and Marshall College
Inventors:
Ryan A. Mehl, Krzysztof Matyjaszewski, Saadyah Averick
Abstract: The present invention provides a method of producing lysergic acid and other ergot alkaloids by genetic modification of a fungus. A strain of fungus comprising Aspergillus fumigatus (A. fumigatus) and expressing one or more genes of the ergot alkaloid biosynthesis pathway from one or more fungus selected from the group consisting of Epichloë festucae var. lolii×Epichloë typhina isolate Lp1 (E. sp. Lp1); Claviceps species; Claviceps africana (C. africana); Claviceps gigantea (C. gigantea); Epichloë coenophiala and Periglandula species, wherein gene easA or gene easM is inactivated in said A. fumigatus, is provided.
Type:
Grant
Filed:
June 15, 2015
Date of Patent:
October 17, 2017
Assignee:
West Virginia University
Inventors:
Daniel G. Panaccione, Sarah L. Robinson
Abstract: A genetically modified yeast cell that is capable of consuming glucose at an increased rate and a method of efficiently producing pyruvate or pyruvate-derived products by using the yeast cell.
Type:
Grant
Filed:
May 27, 2015
Date of Patent:
October 10, 2017
Assignees:
SAMSUNG ELECTRONICS CO., LTD., SNU R&DB FOUNDATION
Inventors:
Jiyoon Song, Jisook Hahn, Changduk Kang, Daehee Kim, Kwangmyung Cho
Abstract: Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone.
Abstract: The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase from Escherichia coli and other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.
Type:
Grant
Filed:
November 12, 2013
Date of Patent:
September 19, 2017
Assignee:
Feed Research Institute, Chinese Academy of Agricultural Sciences
Inventors:
Bin Yao, Huoqing Huang, Huiying Luo, Chao Shao, Yingguo Bai, Yaru Wang, Peilong Yang, Pengjun Shi, Kun Meng, Heng Zhao, Rui Ma
Abstract: The present invention relates to pharmaceuticals and modified beta-lactamases. Specifically, the invention relates to novel recombinant beta-lactamases and pharmaceutical compositions comprising the beta-lactamases. Also, the present invention relates to methods for modifying a beta-lactamase, producing the beta-lactamase and treating or preventing beta-lactam antibiotic induced adverse effects. Furthermore, the present invention relates to the beta-lactamase for use as a medicament and to the use of the beta-lactamase in the manufacture of a medicament for treating or preventing beta-lactam antibiotics induced adverse effects. Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.
Type:
Grant
Filed:
April 26, 2016
Date of Patent:
September 19, 2017
Assignee:
SYNTHETIC BIOLOGICS, INC.
Inventors:
Pertti Koski, Ulla Airaksinen, Katja Valimaki
Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogs, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
Abstract: The present invention relates to a method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from mammalian blood/use of a device comprising a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, for extracorporeal removal of said pathogenic microbe, inflammatory cell or inflammatory protein from mammalian blood/use of a carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, wherein said carbohydrate is immobilized on a solid substrate, in the preparation of a device for treatment of a condition caused or aggravated by said pathogenic microbe, inflammatory cell or inflammatory protein and a method for treatment of a mammalian subject suffering from a condition caused or aggravated by a pathogenic microbe, an inflammatory cell or an inflammatory protein.
Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.
Abstract: Provided is an acid-resistant yeast cell that is genetically engineered to enhance activity of a radiation sensitivity complementing kinase, and a method of producing lactate by using the yeast cell.
Type:
Grant
Filed:
July 23, 2015
Date of Patent:
September 12, 2017
Assignee:
SAMSUNG ELECTRONICS CO., LTD.
Inventors:
Huisub Lim, Sungsoo Kim, Jiyoon Song, Soyoung Lee, Juyoung Lee, Kwangmyung Cho
Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
Type:
Grant
Filed:
February 22, 2013
Date of Patent:
September 12, 2017
Assignee:
Roal OY
Inventors:
Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Hooman, Sanni Voutilainen, Teemu Halonen, Liisa Viikari