Abstract: Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.
Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Pestalotiopsis virgatula.
Type:
Grant
Filed:
October 8, 2013
Date of Patent:
November 22, 2016
Assignee:
Novozymes A/S
Inventors:
Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Pestalotiopsis virgatula.
Type:
Grant
Filed:
October 8, 2013
Date of Patent:
November 22, 2016
Assignee:
Novozymes A/S
Inventors:
Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
Type:
Grant
Filed:
August 6, 2015
Date of Patent:
November 15, 2016
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Brian Brazeau, Jin-Sook Chang, Kwang Myung Cho, Young Wook Cho, Mervyn Desouza, Holly J. Jessen, So-Young Kim, Wei Niu, Fernando A. Sanchez-Riera, Yong-Uk Shin, Hyewon Um
Abstract: The invention provides novel mutant thermostable DNA polymerases having desirable properties such as increased polymerase efficiency and speed, increased affinity to DNA substrates and/or increased resistance to DNA polymerase inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel DNA polymerases.
Type:
Grant
Filed:
August 2, 2012
Date of Patent:
November 15, 2016
Assignee:
Thermo Fisher Scientific Baltics UAB
Inventors:
Remigijus Skirgaila, Agne Tubelevi{hacek over (c)}iūté, Renata Rim{hacek over (s)}eliėnė, Sigitas Burinskas
Abstract: The process for the production of the yellow pigment can include the steps of (a) culturing Bacillus sp. GSK07 bacteria; and (b) extracting the yellow pigment from the bacterial culture using a solvent. The solvent for extraction can be an alcohol, e.g., ethanol, methanol, or both ethanol and methanol. The pigment includes D-limonene.
Type:
Grant
Filed:
December 16, 2015
Date of Patent:
November 15, 2016
Assignee:
KING SAUD UNIVERSITY
Inventors:
George Seghal Kiran, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Joseph Selvin
Abstract: The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.
Type:
Grant
Filed:
February 18, 2015
Date of Patent:
November 15, 2016
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Andrew C. Eliot, Caroline M. Peres, Dmitrii V. Vaviline
Abstract: The present invention refers to novel dimeric proteins obtained from modified ubiquitin capable of binding targets with high affinity. The novel dimeric binding proteins comprise a combination of amino acid substitutions and at least one insertion of amino acids in one of the monomers. The invention is further directed to the use of said proteins in medical diagnosis or treatment methods.
Type:
Grant
Filed:
June 15, 2012
Date of Patent:
November 15, 2016
Assignee:
Scil Proteins GmbH
Inventors:
Joerg Nerkamp, Eva Bosse-Doenecke, Arnd Steuernagel, Ulrike Fiedler, Markus Fiedler
Abstract: A composition including protein hydrolysates derived from milk is obtained by the treatment of enzymes derived from microorganisms. A trypsin-like enzyme and a chemotrypsin-like enzyme are used. The composition is in particular intended for inducing tolerance in infants with the effect of modulating the potential occurrence of allergies later in life. The composition can also be used in sick adult patients. Preferably the composition is an infant formula, infant follow-up formula, growing-up milk or baby food or an enteral complete nutritional composition.
Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Grant
Filed:
August 10, 2012
Date of Patent:
November 8, 2016
Assignee:
Novozymes A/S
Inventors:
Sara Landvik, Lars H. Oestergaard, Lisbeth Kalum
Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.
Type:
Grant
Filed:
May 15, 2015
Date of Patent:
November 8, 2016
Assignee:
REGENTS OF THE UNIVERSITY OF MICHIGAN
Inventors:
David H. Sherman, Michael Marie Kaufman-Schofield, Sunit Jain, Gregory Dick
Abstract: The invention relates to new method of peptide hydrolysis, in particular of the cell walls of Gram-positive bacteria, wherein the active form of LytM or derivative thereof is contacted with a peptide substrate, preferably with the cell walls of Gram-positive bacteria, in an aqueous environment of conductivity lower than 10 mS/cm. The invention also relates to composition comprising active form of LytM or derivative thereof and new uses of active form of LytM or derivative thereof.
Type:
Grant
Filed:
October 17, 2013
Date of Patent:
November 8, 2016
Assignee:
MIEDZYNARODOWY INSTYTUT BIOLOGII MOLEKULARNEJ I KOMORKOWEJ
Abstract: Methods and compositions relating to the engineering of an improved protein with methionine-?-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-?-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.
Type:
Grant
Filed:
August 29, 2014
Date of Patent:
November 1, 2016
Assignee:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Inventors:
George Georgiou, Everett Stone, Wei-Cheng Lu
Abstract: The present invention relates to repetitive self-assembling precursor proteins, nucleic acid sequences and expression constructs encoding the same, and to methods for recombinant production of peptides using such precursor proteins.
Type:
Grant
Filed:
June 2, 2010
Date of Patent:
November 1, 2016
Assignee:
BASF SE
Inventors:
Daniel Hümmerich, Burghard Liebmann, Marcus Fehr, Carsten Schwalb, Heike Brüser
Abstract: Yeast strains capable of increased prenyl phosphate production are provided, enabling increased terpenoid molecule production. Heterologous yeast strains with high prenyl phosphate availability are prepared using one or both of two different strategies for increasing the availability of prenyl phosphates for terpenoid production. First, by co-expressing multiple mevalonate pathway gene analogs, a novel heterologous combination of genes results, some of which increases the inherent availability of prenyl phosphates in yeast. Second, by expressing the non-endogenous enzyme ATP citrate lysase (ACL), a buildup of high cytosolic concentration of acetyl-CoA is produced in the cytosol of S. cerevisiae.
Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
Type:
Grant
Filed:
March 3, 2015
Date of Patent:
October 25, 2016
Assignee:
Codexis, Inc.
Inventors:
Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
Abstract: The present invention relates to methods of use of glycosyltransferases and related novel compounds. The invention exploits the reversibility of glycosyltransferases to generate new sugars, unnatural biomolecules and numerous one-pot reactions for generation of new biomolecules having varied backbones such as enediynes, vancomycins, bleomycins, anthracyclines, macrolides, pluramycins, aureolic acids, indolocarbazoles, aminglycosides, glycopeptides, polyenes, coumarins, benzoisochromanequinones, calicheamicins, erythromycin, avermectins, ivermectins, angucyclines, cardiac glycosides, steroids or flavinoids. In preferred embodiments, the invention specifically relates to biosynthesis of anticancer (the enediyne calicheamicin, CLM), anthelmintic agents (the macrolides avermectin, ivermectin and erythromycin) and antibiotic (the glycopeptide vancomycin, VCM) natural product-based drugs developed by reversible, bidirectional glycosyltransferase-catalyzed reactions.
Type:
Grant
Filed:
July 18, 2014
Date of Patent:
October 18, 2016
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventors:
Jon S. Thorson, Changsheng Zhang, Byron R. Griffith
Abstract: This invention provides methods and systems for the production of butanol. Specifically, the methods and systems of the present invention use syntrophic co-cultures for the production of butanol from syngas.
Type:
Grant
Filed:
January 18, 2013
Date of Patent:
October 18, 2016
Assignee:
SYNATA BIO, INC.
Inventors:
Mike Enzien, Rathin Datta, Richard Tobey
Abstract: The present invention relates to a method for producing an L-amino acid by reacting an enantiomeric mixture of an N-succinyl amino acid with L-succinylase in the presence of N-acylamino acid racemase to specifically hydrolyze the L-form. In particular, the present invention relates to a method for producing an L-amino acid in high yield by using an N-succinyl amino acid whose dissolved concentration is particularly low as a raw material to perform a reaction while precipitating the produced L-amino acid out of the reaction system. The present invention enables efficient production of an L-amino acid having high optical purity, particularly an L-amino acid useful as a raw material for products such as pharmaceutical products and agricultural chemicals.