Patents Examined by Robert Mondesi
  • Flavin-binding glucose dehydrogenase
    Patent number: 8945359
    Abstract: The invention provides a flavin-binding glucose dehydrogenase exhibiting reduced fluctuation of activity depending on temperature environment, and a method for measuring glucose concentration using the flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase has the following properties (1) to (3): (1) activity: which exhibits glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: which exhibits an activity of 10% or less against maltose, D-galactose, D-fructose, sorbitol, lactose and sucrose when the activity against D-glucose is defined as 100%; and (3) temperature characteristics: which exhibits lower fluctuation of activity in a wide temperature range of 10 to 50° C.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: February 3, 2015
    Assignee: Ikeda Food Research Co., Ltd.
    Inventors: Michinari Honda, Sayaka Taki, Ryo Takenaka
  • Modified beta-lactamases and methods and uses related thereto
    Patent number: 8894994
    Abstract: The present invention relates to pharmaceuticals and modified beta-lactamases. Specifically, the invention relates to novel recombinant beta-lactamases and pharmaceutical compositions comprising the beta-lactamases. Also, the present invention relates to methods for modifying a beta-lactamase, producing the beta-lactamase and treating or preventing beta-lactam antibiotic induced adverse effects. Furthermore, the present invention relates to the beta-lactamase for use as a medicament and to the use of the beta-lactamase in the manufacture of a medicament for treating or preventing beta-lactam antibiotics induced adverse effects. Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.
    Type: Grant
    Filed: October 7, 2013
    Date of Patent: November 25, 2014
    Assignee: Synthetic Biologics, Inc.
    Inventors: Pertti Koski, Ulla Airaksinen, Katja Valimaki
  • Transaminase biocatalysts
    Patent number: 8889380
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: September 5, 2012
    Date of Patent: November 18, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Emily Mundorff, Jeffrey Moore, Paul N. Devine, Jacob M. Janey
  • Enone reductases
    Patent number: 8883475
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 23, 2012
    Date of Patent: November 11, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
  • Method for producing multicyclical ring systems carrying amino groups
    Patent number: 8841096
    Abstract: The invention relates to a method for the amination of at least one keto group in a multicyclic ring system comprising at least one keto group into an amino group, using at least one enzyme E having transaminase activity.
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: September 23, 2014
    Assignee: Evonik Degussa GmbH
    Inventors: Volker Sieber, Katrin Grammann, Broder Ruehmann, Thomas Haas, Jan Christoph Pfeffer, Kai Doderer, Claudia Rollmann, Arne Skerra, Christian Rausch, Alexandra Lerchner
  • Yellow pigments generation deficient strain and application thereof in gellan gum production
    Patent number: 8685698
    Abstract: A yellow pigments generation deficient Sphingomonas strain (Sphingomonas sp. ZD001) and application thereof in preparing gellan gum by microbial fermentation are provided. The strain is preserved in China Center for Type Culture Collection (CCTCC) with the address of Wuhan University, Wuhan, 430072, China, the preservation date of the strain is 10 Sep. 2009, and the preservation serial number is CCTCC No: M 209298. The main beneficial effect of the present invention is that the fermented liquor contains no yellow pigments but is milky white, colorless superior gellan gum can be obtained by only depositing polysaccharide with a small quantity of ethanol or isopropanol, so that the post purification and de-coloration techniques of gellan gum production can be simplified, the yield can be enhanced, and the production cost can be reduced.
    Type: Grant
    Filed: August 13, 2010
    Date of Patent: April 1, 2014
    Assignees: Zhejiang University, Zhejiang DSM Zhongken Biotechnology Co., Ltd.
    Inventors: Xuechang Wu, Rongming Wu, Ou Li, Liang Zhu, Yamin Chen, Chaodong Qian, Mei Chen
  • Xylose utilization in recombinant zymomonas
    Patent number: 8679822
    Abstract: Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.
    Type: Grant
    Filed: June 16, 2011
    Date of Patent: March 25, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Perry G. Caimi, Laura McCole, Luan Tao, Jean-Francois Tomb, Paul V. Viitanen
  • Thermostabilization of proteins
    Patent number: 8637009
    Abstract: Provided are compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes the CocE. Also provided are methods of thermostabilizing a cocaine esterase. Additionally provided are methods of treating a mammal undergoing a cocaine-induced condition. Methods of determining whether a compound is a thermostabilizing agent for a protein are also provided. Uses of the above-described compositions for the treatment of a cocaine-induced condition is additionally provided. Additionally provided is an isolated nucleic acid encoding a CocE polypeptide having the substitutions L169K and G173Q, and the CocE polypeptide encoded by that nucleic acid, and pharmaceutical compositions thereof. Further provided is the use of that composition for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition.
    Type: Grant
    Filed: July 10, 2008
    Date of Patent: January 28, 2014
    Assignees: The Trustees of Columbia University in the City of New York, The Regents of the University of Michigan
    Inventors: Donald W. Landry, James H. Woods, Roger K. Sunahara, Diwahar L. Narasimhan, Joanne MacDonald, Milan N. Stojanovich, John J. Tesmer, Remy L. Brim
  • Variant CBH1 cellulases
    Patent number: 8637294
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: March 22, 2007
    Date of Patent: January 28, 2014
    Assignee: Danisco US Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Lipases with high specificity towards short chain fatty acids and uses thereof
    Patent number: 8637101
    Abstract: The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well as functional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.
    Type: Grant
    Filed: February 26, 2009
    Date of Patent: January 28, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Jan Metske Laan Van Der, Yulia M. Efimova, Karin Turk, Margot Elisabeth Francoise Schooneveld-Bergmans, Arie Gerrit Terdu, Arjen Sein, Natalja Alekseevna Cyplenkova
  • Method of producing proteins
    Patent number: 8597907
    Abstract: The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5?-end to 3?-end, and secretory producing the heterologous protein by coryneform bacteria.
    Type: Grant
    Filed: October 19, 2006
    Date of Patent: December 3, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masayo Date, Yoshimi Kikuchi, Hiroshi Itaya, Nami Nakamura
  • Peptidase substrates
    Patent number: 8592174
    Abstract: The present invention relates to the use of a compound of the following formula (I), as an enzyme substrate for the detection of a peptidase activity or as a pH indicator: according to which: Y1 is a peptide, H or an alkyl; W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination thereof; n=0, 1 or 2; U is N or N+R and V is CZ6N or N+R or else V is N or N+R and U is CZ6; R is H, alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl; Z1, Z2, Z3, Z4 and Z5 are independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulfonyl, including the carboxyl or sulfonyl esters or amides, and salts thereof.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: November 26, 2013
    Assignee: bioMérieux, S.A.
    Inventors: Olivier Fabrega, Arthur James, Sylvain Orenga, John Perry, Vindhya Salwatura, Stephen Stanforth
  • Nitroreductase enzymatic substrates
    Patent number: 8592175
    Abstract: The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; U is N or N+R and V is CZ6N or N+R or V is N or N+R and U is CZ6, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; and Z1, Z2, Z3, Z4, Z5 and Z6 are independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same.
    Type: Grant
    Filed: July 29, 2010
    Date of Patent: November 26, 2013
    Assignee: bioMérieux, S.A.
    Inventors: Olivier Fabrega, Arthur James, Sylvain Orenga, John Perry, Vindhya Salwatura, Stephen Stanforth
  • Immobilized TAL biocatalyst for production of para-hydroxycinnamic acid
    Patent number: 8569036
    Abstract: TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.
    Type: Grant
    Filed: July 12, 2006
    Date of Patent: October 29, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Robert Dicosimo, Sharon L. Haynie, Lixuan Lisa Huang, Fateme Sima Sariaslani, Tina K. Van Dyk, Robert J. Trotman
  • Surfactant tolerant cellulase and method for modification thereof
    Patent number: 8569033
    Abstract: A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.
    Type: Grant
    Filed: December 7, 2004
    Date of Patent: October 29, 2013
    Assignee: Meiji Seika Pharma Co., Ltd.
    Inventors: Manabu Watanabe, Koji Yanai, Yumiko Tsuyuki
  • Endometriosis-related markers and uses thereof
    Patent number: 8541173
    Abstract: The present invention relates to markers of endometriosis which are differentially expressed in the endometrial cells of females with endometriosis compared to endometriosis-free females. The invention also relates to methods for determining likelihood of endometriosis in female subjects, to methods for grading endometriosis in females suffering from endometriosis and to methods for treating this disease. The invention is also concerned with polynucleotides, probes, primers and kits useful for reducing into practice the above-mentioned methods which are more rapid, non invasive, much less complicated and much less costly than laparoscopy.
    Type: Grant
    Filed: February 23, 2012
    Date of Patent: September 24, 2013
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Soheyl Baban, Monique Bernard, Elana Cherry, Diane Gosselin, Patrice Hugo, Brigitte Malette, Pierre Miron, Charles Privé, Kamran Shazand
  • Process for the production of fine chemicals
    Patent number: 8541208
    Abstract: The present invention relates to a process for the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The present invention relates further to a process for the control of the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
    Type: Grant
    Filed: December 21, 2006
    Date of Patent: September 24, 2013
    Assignee: Metanomics GmbH
    Inventors: Gunnar Plesch, Piotr Puzio, Astrid Blau, Ralf Looser, Birgit Wendel, Beate Kamlage, Oliver Schmitz
  • Peptidase substrates
    Patent number: 8535902
    Abstract: The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: according to which: Y1 is a peptide, H or an alkyl; W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination thereof; n=0, 1 or 2; X is NR, CZ5Z6, S or O, R being H, alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl, Z5 and Z6 being an alkyl; Y is N or N+R, R being alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl; Z1, Z2, Z3 and Z4 being independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulfonyl, including the carboxyl or sulfonyl esters or amides, and salts thereof.
    Type: Grant
    Filed: July 27, 2010
    Date of Patent: September 17, 2013
    Assignee: bioMërieux, S.A.
    Inventors: Olivier Fabrega, Arthur James, Sylvain Orenga, John Perry, Vindhya Salwatura, Stephen Stanforth
  • Three-dimensional structure of isoprene synthase and its use thereof for generating variants
    Patent number: 8518686
    Abstract: The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
    Type: Grant
    Filed: April 22, 2010
    Date of Patent: August 27, 2013
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Quinn Beck, Richard R. Bott, Christopher Lee Rife, Derek H. Wells, Jeffrey V. Miller
  • Enzyme for the production of long chain peracid
    Patent number: RE44648
    Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some embodiments, the present invention provides methods and compositions for generation of long chain peracids. Certain embodiments of the present invention find particular use in applications involving cleaning, bleaching and disinfecting.
    Type: Grant
    Filed: June 15, 2012
    Date of Patent: December 17, 2013
    Assignee: Danisco US Inc.
    Inventors: Neelam S. Amin, Richard R. Bott, Marguerite A. Cervin, Ayrookaran J. Poulose, Walter Weyler