Abstract: The present invention provides a method for making whole cells of methylotrophic species of genus Pichia competent for transformation by DNA and a method for transforming with DNA whole cells of such species.

Abstract: A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50.degree. C. to 85.degree. C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50.degree. to 85.degree. C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, coverting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time.

Abstract: A method for the solubilization and recovery of insoluble parasite protective antigenic factors associated with parasite material comprising solubilizing the antigenic factors with a non-ionic detergent and separating the solubilized material from undispersed residual material. The purified protective antigenic factors are useful as vaccines, particularly against malaria, and as diagnostic agents.

Abstract: A method for controlling the level of heterologous gene expression in yeast using a soybean leghemoglobin leader sequence and a suitable promotor. Levels of expression are controlled at the post-transcriptional level by adjusting the amount of heme in the yeast culture medium.

Abstract: A process for producing a yeast capable of heat regulated synthesis of selected and fused proteins is disclosed. The process involves the combination of a DNA fragment comprising a heat shock inducible gene of an appropriate yeast microorganism and a second DNA fragment comprising a selected gene with a suitable transfer vector, thereby producing a recombinant transfer vector. A suitable host yeast is contacted with the recombinant transfer vector, cultured and transforments having the desired genetic capability are selected.

Abstract: An immunopropylactic and immunotherapeutic agent comprising human interleukin 2 of human cellular origin is disclosed along with a method of producing the agent.

Abstract: This invention relates to proteolytic enzymes with elastolytic properties from blood-sucking nematodes (hereinafter called HP enzymes). These enzymes are useful an anti-coagulants and as a means for dissolving fibrin clots. Because of their importance in the mechanism by which blood-sucking nematodes obtain nutrients from the host, HP enzymes would be an effective vaccine for prevention and treatment of these parasites. For the same reason, substances which inhibit HP enzymes would also be effective antithelminic agents.

Abstract: A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Sample; a polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex are brought together in a medium. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentration of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte to be directly related to the conversion of the substrate to the reporter molecule.

Abstract: A process for the preparation of optically pure D- or L-lactic acid by fermentation of an aqueous nutrient medium, which contains nitrogen, vitamins, aminoacids, sugars and trace elements, by means of a microorganism, at pH 4-6, wherein the nutrient medium contains brewers' yeast as the source of nitrogen, vitamins, aminoacids and trace elements.

Abstract: The present invention relates to drugs characterized in that they comprise, in association, at least one immunotoxin and at least one monovalent carboxylic ionophore.

Abstract: Ice nucleation bacteria are modified in vitro to confer an ice nucleation deficient phenotype. Modification is accomplished by deletion, substitution, insertion, inversion, or transversion of a DNA segment within the gene locus responsible for the INA phenotype. By limiting such mutations to the particular gene locus, the modified microorganisms are genetically stable and free from random mutations which might adversely affect their competitive fitness. The modified microorganisms are useful for prevention of frost damage to susceptible plant hosts.

Abstract: An expression vector, a plasmid containing yeast alcohol dehydrogenase I promoter and terminator and the gene coding for the rat liver cytochrome P-450MC gene Saccharomyces cerevisiae transformed with the plasmid and process for preparing rat liver cytochrome P-450MC.

Abstract: A lipid coupling reagent for use in coupling amine-containing molecules, such as proteins, to liposomes. The reagent includes phosphatidylethanolamine coupled to a 3-20 atom carbon-containing spacer arm terminating at a carboxyl or thiocarboxyl group. Also disclosed are liposomes prepared to include between about 1 and 20 mole percent of the coupling reagent, and liposomes containing surface arrays of proteins attached to the liposomes through the coupling reagent.

Abstract: This invention is directed to the production of plants, plant tissues and plant seeds which are resistant to inhibition by an herbicide which normally inhibits the growth and development of those plants, plant tissues and plant seeds. In particular this invention is directed to altered acetohydroxyacid synthase enzymes which are resistant to inhibition by herbicides which normally inhibit the activity of the synthase before such alteration. This invention further relates to genes encoding such enzymes, and to processes for utilizing these novel genes and enzymes. Further products of the invention include plants, plant tissues and seeds which exhibit resistance to such herbicides resulting from expression of genes encoding herbicide resistant acetohydroxyacid synthase enzyme.

Abstract: Methods and compositions are provided for regulated expression and secretion of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with four functional fragments derived from the lipoprotein gene of E. coli. The plasmids also include a DNA fragment coding for the signal peptide of the ompA protein of E. coli, positioned such that the desired polypeptide is expressed with the ompA signal peptide at its amino terminus, thereby allowing efficient secretion across the cytoplasmic membrane. The plasmids further include a DNA sequence coding for a specific segment of the E. coli lac promoter-operator, which is positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate functional E.

Abstract: Methods and compositions are provided for high level production of foreign proteins in yeast. The system is exemplified by production of .alpha..sub.1 -antitrypsin in yeast strain AB110, derived from yeast strains 2150-2-3 and AB103.1.S. carlsbergensis/S. cerevisiae hybrid strain AB110 containing plasmid pCl/1GAPATi9 was deposited at the A.T.C.C. on May 9, 1984, and given Accession No. 20709.

Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S.

Abstract: Biological compositions are freed of functional polynucleotides by treatment of the biological composition with psoralen derivatives under irradiation conditions in which the proteins retain their original physiological activities and any polynucleotide present is rendered inactive.

Abstract: A biological control agent comprising the antifungal agent Trichoderma harzianum T-35 (ATCC No. 20691), which is characterized by antifungal activity against fungi of the genus Fusarium. This strain is useful for protecting most crops affected by the fungus Fusarium spp. and is more active than other previously disclosed strains. Biocontrol compositions containing T. harzianum T-35 (ATCC No. 20691) provide antifungal protection to a broad spectrum of plants, including wheat, cotton, melons and tomatoes.

Abstract: It has previously been shown that the serum from patients suffering from a wide range of cancers contains a cancer marker protein having the ability to release RNA from cell nuclei. This cancer marker protein is purified by taking the protein fraction precipitating between 30% and 50% saturated aqueous ammonium sulfate solution, dialyzing a solution of the protein fraction against TMK buffer, chromatographing the dialyzed protein on a molecular sieve and isolating the fraction having a molecular weight of about 60,000; and removing albumin. Injection of the purified protein into rabbits, preparation of serum from blood of the rabbits and absorption of the sera with normal plasma fraction produces at antibody specific to the cancer marker protein and therefore useful in a radioimmunoassay or ELISA test for a wide variety of cancers.