Abstract: Disclosed is an autoreplicative plasmid containing DNA which encodes herbicide resistance, such that the plasmid is capable of transforming a yeast cell to become resistant to one or more organic herbicide growth inhibitors. Also disclosed is a process for the selective culture of yeasts transformed by the plasmid.

Abstract: Methods and compositions are provided for the recombinant synthesis of the tumor growth factor-.alpha. precursor and its fragments. These are useful in therapy and diagnosis, as are antibodies raised by immunization with the tumor growth factor-.alpha. precursor and its fragment.

Abstract: This invention provides a process for producing nylon 6,6 salt which involves bioconversion of toluene to muconic acid in the presence of hexamethylenediamine to yield hexamethylenediamine muconate salt. Hydrogenation of this salt provides an aqueous solution of the desired hexamethylenediamine adipate salt.

Abstract: L-histidine is produced by culturing, in a nutrient medium, an L-histidine producing mutant microorganism belonging to the genus Corynebacterium. The mutant is resistant to a precursor for ubiquinone biosynthesis. L-histidine is accumulated in the culture liquor and is recovered therefrom.

Abstract: An intravenous immune globulin preparation having at least 99% pure globulin protein and an anticomplement activity of less than 0.10 C'50 units/mg IgG prepared by: precipitating impurities from Cohn Fraction II in an aqueous-alcohol medium at defined temperature and pH, removing the precipitated impurities, stabilizing the diluted solution with albumin, concentrating the solution and removing the alcohol therefrom. Also prepared by this method, an intravenous, hyperimmune globulin preparation having increased antibody titers to sixteen serospecific strains of Pseudomonas aeruginosa.

Abstract: A selective method of suppressing the growth of cells which express a viral antigen on the surface thereof, which comprises administering to the cells a growth suppressing amount of a monoclonal antibody against said viral antigen, especially a method of suppressing the growth of hepatocytes or hepatoma cells persistently infected with HBsAg which comprises administering to the cells a growth suppressing or lethal amount of a complement fixing monoclonal IgM or IgG.sub.2a antibody against HBsAg.

Abstract: This invention relates to the field of lignin degradation. More specifically the invention relates to methods for selecting and isolating microorganism from nature that are capable of degrading lignin, processes for cloning a gene segment from such an organism, and methods of using the enzyme product of the gene segment to provide valuable chemical feedstocks, methanol and the like from a lignin source material.

Abstract: The invention discloses a method for producing modified signal peptides sequences derived from wild-type signal peptide sequences of the type that are capable of forming membrane-bound lipoproteins. Modified signal peptide sequences produced by the method of the invention are useful for increasing the secretion of heterologous gene products produced by transformed host organisms. The invention further discloses a method for producing recombinant DNA sequences in vivo.

Abstract: A new class of chemical reagents called release tags which comprise signal, release and reactivity groups is disclosed and a release tag involving a pentafluorobenzoyl signal group, a methionylamide release group, and an active ester reactivity group is used to analyze the hormone, thyroxine, in serum, involving quantitation of the released signal group by gas chromatography with electron capture detection.

Abstract: A method of producing a non-heme haloperoxidase which is substantially resistant to inactivation, at room temperature, in up to 0.3M H.sub.2 O.sub.2 for up to 25 hours, and up to 0.5 mM HOCl for up to two minutes. One such haloperoxidase, isolated from Curvularia inaequalis, contains about 2 gram atoms of zinc per molecule. A halogenation reaction employing the enzyme can be performed at H.sub.2 O.sub.2 and hypohalous acid concentrations which produce rapid inactivation of heme-containing haloperoxidases.

Abstract: A halogenation method using a haloperoxidase obtained from a fungus selected from the dematiaceous hyphomycetes. The enzyme has an optimum activity above about pH 5.0, and can oxidize chloride, bromide, or iodide ions.

Abstract: By coupling an antigen to a polyphenol derivative the formation of the corresponding antibody can be selectively suppressed by administration thereof to the desired subject.

Abstract: New angiogenic factors of low molecular weight (under 1000), useful as treatment agents for wound healing and for biological assaying, made from in vitro cultures of a wide variety of tumor cells by a treatment which breaks up the proteinaceous tumor angiogenesis factors ("TAF") in them and liberates the more mobile low molecular weight factors. The treatment can be applied to cell extracts or the aqueous portion of the culture, and the proteinaceous part may be rendered insoluble or captured chemically so as to release the new factor. Separation techniques which can be used include solvent extraction of dried liquid fractions, using polar solvents such as ethanol, and the liberated factor can be purified chromatographically using adsorbents for which the factor has affinity, especially a basic adsorbent and preferably polysaccharide-based, followed by elution.

Abstract: Methods and compositions are provided for preparing and using pesticides, where the pesticides are encapsulated in non-proliferating cells. The methods involve introducing a heterologous gene into a cellular host, where expression of the heterologous gene results, directly or indirectly, in production of the pesticide. These cells are then killed under conditions which prolong the pesticidal activity when said cells are applied to the environment of a target pest. The killed cells can be used directly or after formulation for treatment of an agricultural host or environment of the host with the pesticide.

Abstract: The invention relates to a process for the incorporation of foreign DNA into the genome of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium tumefaciens bacteria, which contain one or more Ti (tumour inducing) plasmids, wherein as Ti plasmid a stable cointegrate plasmid composed of the plasmid R772 and the plasmid pTiB6 with foreign DNA incorporated in the T-region of the Ti component of the cointegrate plasmid is applied as well as to a cointegrate plasmid pAL969 and cointegrate plasmids derived from the cointegrate plasmid by the incorporation of foreign DNA into the T-region of the Ti component.

Abstract: Method for transforming anaerobic microorganisms. Anaerobic microorganisms are induced to form L-forms. Genetic material capable of inducing the desired phenotype is introduced into the L-forms, after which the L-forms may be caused to regenerate their cell wall. These methods are also useful for obtaining desired biological products. Additionally, shuttle vectors capable of transforming both aerobic and anaerobic microorganisms are set forth.

Abstract: Disclosed are novel DNA sequences operative as promoters of microbial transcription of structural genes and comprising synthetic E.coli bacteriophage T5 "early" promoter replica DNA sequences. Disclosed also are novel promoter/operator DNA sequences comprising a synthetic T5 early promoter replica DNA sequence associated with a regulatable operator DNA sequence providing for selectively regulatable transcription of structural genes in, e.g., E.coli cells transformed with a DNA vector including same.

Abstract: Interleukin-2 dependent cytotoxic cultured T-cell lines are found to produce .alpha., .beta., and .gamma. interferon, as well as interleukin-2, when stimulated by mitogenic or antigenic agents.

Abstract: A method for treating anestrus in ewes and beef cattle by treating a ewe or a beef cow for 4 to 16 days with a gestagen having protracted action, followed by administering LH-RH or an analogous compound of at least the same efficiency for 2 to 6 days to bring about an LH plasma level sufficient to induce ovulation.

Abstract: Antiviral proteins phytolaccin.sub.1 and phytolaccin.sub.2 were isolated from the higher plant Phytolaccin americana using the technique of immunoaffinity chromatography. Phytolaccin proteins were purified to apparent homogeneity in a rapid and efficient chromatographic procedure utilizing immobilized monospecific anti-phytolaccin antibodies. Immunoaffinity-purified phytolaccin.sub.1 and phytolaccin.sub.2 were determined by denaturing gel electrophoresis to be of approximately 25,600 and 28,400 daltons, respectively. The two immunoaffinity-purified proteins were equally potent inhibitors of eukaryotic cell-free protein biosynthesis and exhibited mostly similar high-order UV derivative spectra. Antibodies against phytolaccin.sub.1 and phytolaccin.sub.2 did not cross-react with the heterologous antigen, indicating a structural dissimilarity between the two phytolaccin proteins.Phytolaccin.sub.