Abstract: To determine the presence of Mycobacterium in an environment, a sample from the environment can be plated onto a growth medium that is selective for Mycobacterium. The agar based growth medium can include a high concentration of crystal violet, in excess of 0.5 ?g/ml. The process may be made further selective for Mycobacterium by treating the sample with sodium dodecyl sulfate containing glycine hydrochloride for at least 4 minutes at room temperature, prior to plating. Mycobacterium colonies will generally appear white while other colonies will generally appear stained purple or another color.

Abstract: Disclosed herein is a method for alleviating atopic dermatitis using a composition containing at least one lactic acid bacterial strain. The at least one lactic acid bacterial strain is selected from the group consisting of Lactobacillus salivarius subsp. salicinius AP-32 which is deposited at the China Center for Type Culture Collection (CCTCC) under an accession number CCTCC M 2011127, and Bifidobacterium animalis subsp. lactis CP-9 which is deposited at the CCTCC under an accession number CCTCC M 2014588.

Abstract: Disclosed herein are compositions and methods for treating periodontal disease.

Abstract: A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.

Abstract: A probiotic composition of improving memory and learning ability is disclosed. The probiotic composition comprises Lactobacillus acidophilus GMNL-185 and Lactobacillus rhamnosus GMNL-680, wherein the Lactobacillus acidophilus GMNL-185 was deposited in the China Center for Type Culture Collection in Wuhan, China on Nov. 3, 2017 under an accession number CCTCC NO. M 2017764, and the Lactobacillus rhamnosus GMNL-680 was deposited in the China Center for Type Culture Collection in Wuhan, China on Nov. 3, 2017 under an accession number CCTCC NO. M 2017766, wherein a ratio of a number of bacteria of the Lactobacillus acidophilus GMNL-185 to a number of bacteria of the Lactobacillus rhamnosus GMNL-680 is 1:1.

Abstract: The present disclosure relates to as external composition for wound healing containing a Lactobacillus fermentation product, which comprises a Lactobacillus fermentation product as an effective component. The Lactobacillus fermentation product is a bacteria-free concentrated filtrate from fermentation of Lactobacillus plantarum and the effective component is loaded onto a pharmaceutically acceptable absorbent carrier or carrying agent. The external composition for wound healing has anti-inflammatory and healing promoting effects on a skin wound after applied directly onto the skin wound.

Abstract: A strain of Oceanobacillus picturae and applications thereof are provided, which relate to technical fields of microorganism and food processing. A strain of halophilic bacteria was screened from the brine of Xiangyang Pickled Kohlrabi and was identified as the strain of Oceanobacillus picturae by morphology, physical and chemical properties and 16S rRNA sequence. The strain is able to significantly increase the varieties and contents of volatile substances, and decrease the contents of pungent substances in pickled vegetables. The strain of Oceanobacillus picturae can improve the taste and the flavor of the pickled vegetables.

Abstract: The present invention provides a Lactococcus lactis subsp. lactis A32, derived product and use thereof, the present invention falls in the technical field of microorganisms. In the present invention, the Lactococcus lactis subsp. lactis A32 is obtainable by compound mutation on the basis of the original strain L. lactis lxl. Experiments showed that, compared with nisin titre of the original strain L. lactis lxl, the Lactococcus lactis subsp. lactis A32 has high nisin yield, and the nisin titre has been improved for 3˜4 times (over 5066.58 IU/mL); in the meanwhile, the growth curve of the Lactococcus lactis subsp. lactis A32 is slightly longer than the original strain L. lactis lxl and biomass peaks of them are basically consistent. The present invention has laid a foundation for large scale production of nisin.

Abstract: Methods of preventing and treating COVID-19 infection by administering probiotics. The probiotics can be administered via the following methods: fecal transplant, suppository, or orally. The probiotic contains one or more of the following microorganisms: Bifidobacterium, Clostridium, Veillonella, Ruminococcus, and Sutterella.

Abstract: The present invention provides devices, systems, and methods, for performing biological and chemical assays.

Abstract: The present disclosure discloses a process for accelerating, increasing, and stabilizing production of biogas with a high methane content in systems for biodigestion of organic waste. The process comprises: a) obtaining from an anaerobic biodigester (3) a fraction of organic waste equal in weight to K times the weight of the daily organic waste load of the anaerobic biodigester (3), wherein the parameter K is a numeric value selected between 10?3 and 10?1, sending the fraction to one or more acceleration devices (4), retaining the fraction in the one or more acceleration devices (4) until a final concentration (Cf) of the methanogenic bacteria in the fraction is equal to M times an initial concentration (Ci), wherein the parameter M is a numeric value selected between 103 and 108; b) as soon as said final concentration (Cf) is obtained in the fraction, directing said fraction from the acceleration device (4) back to the anaerobic biodigester (3); and c) successively repeating steps a) and b).

Abstract: Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatment of alcohol hangover.

Abstract: Disclosed is a composition for promoting absorption of a poorly water-soluble phytochemical which can significantly improve the intake of a phytochemical into a body, especially a migration speed and/or a migration amount of the phytochemical into a blood. A polysaccharide-containing lactic acid bacterial product, which is produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1251, can significantly enhance the intake of a phytochemical into a body, especially a migration speed and/or a migration amount of the phytochemical into a blood. Thus, the composition for promoting absorption of a phytochemical according to the present invention includes, as an active ingredient, the polysaccharide-containing lactic acid bacterial product. Furthermore, the composition for promoting absorption of a phytochemical is useful as a food additive composition.

Abstract: A method for producing a product related to the specified technology includes adjusting the concentration of cells in a culture vessel to a value of from 3×107 cells/ml to 3×108 cells/ml; in a case in which the average diameter of single cells in the culture vessel is designated as A, adjusting the number proportion of cells having a single cell diameter of 1.4×A or greater in the culture vessel to 5% or less, and adjusting the number proportion of cells having a single cell diameter in the range of A±A/7 to 50% or more.

Abstract: Isolates strains of gastrointestinal bacteria from wolves are provided for use as probiotics. In some embodiments, the isolated strains are for use as probiotics in canine subjects such as domestic dogs. In some embodiments, the isolated strains may be used to treat or prevent intestinal dysbiosis in the subject. Also provided are compositions comprising at least one isolated strain of wolf probiotic bacteria and related methods for making same.

Abstract: An oral composition comprising at least one probiotic bacteria selected from the genera: Lactobacillus, Bifidobacterium, and Bacillus, and ferric pyrophosphate.

Abstract: Disclosed is a composition for promoting absorption of a poorly water-soluble phytochemical which can significantly improve the intake of a phytochemical into a body, especially a migration speed and/or a migration amount of the phytochemical into a blood. A polysaccharide-containing lactic acid bacterial product, which is produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1251, can significantly enhance the intake of a phytochemical into a body, especially a migration speed and/or a migration amount of the phytochemical into a blood. Thus, the composition for promoting absorption of a phytochemical according to the present invention includes, as an active ingredient, the polysaccharide-containing lactic acid bacterial product. Furthermore, the composition for promoting absorption of a phytochemical is useful as a food additive composition.

Abstract: The present disclosure relates to the isolation, identification and characterization of novel sporeforming probiotic strain(s) with NSPase (Non-Starch Polysaccharides-active hydrolases) activity isolated from fish gut microbiota, methods and uses thereof. The sporeforming probiotic strain(s) with NSPase activity now disclosed are able of producing carbohydrate-active enzymes (CAZymes) that hydrolyse non-starch polysaccharides (NSPs) and accesses their potential as probiotics (PRO) for use in particular in aquafeeds.

Abstract: Use of a sugar selected from lactose, fructose and raffinose, for increasing serpin expression in Bifidobacterium longum strain CNCM I-2618.

Abstract: Provided is a method of culturing one or more microorganisms. The method includes culturing one or more microorganisms in a medium comprising crude glycerol at a first concentration level, feeding to the media an additional amount of crude glycerol, once the first concentration of glycerol is reduced to a first threshold level, at a concentration sufficient to achieve the first concentration level, monitoring the crude glycerol concentration until the first concentration level of the crude glycerol is reduced to the first threshold level. The steps may be repeated until a desired microorganism cell density is achieved.