Abstract: A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection subsystem. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.
Abstract: The invention is directed to methods and kits which utilize an immobilized solid support to extract the nucleic acids present in the human plasma sample Immobilized solid support further comprises: a) a pre-treated solid support material b) M-Aminophenylboronic acid (APBA), and c) levoglucosenone. The components and methods for activating solid support and extraction of nucleic acids used in the invention provide for high extraction efficiency and the resultant product is a highly purified plasma which is almost completely free of nucleic acids.
Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
Type:
Grant
Filed:
June 1, 2018
Date of Patent:
June 7, 2022
Assignee:
Bio-Rad Laboratories, Inc.
Inventors:
Darren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
Abstract: The present disclosure relates to a method for amplification and quantitation of a small amount of mutation using a molecular barcode and a blocking oligonucleotide.
Abstract: Disclosed herein are methods, reagent kits, and compositions for performing a bisulfite conversion of DNA directly from a biological sample including a patient's urine sample or a slide-mounted FFPE tissue sample. For example, a method of performing a bisulfite conversion of DNA can comprise certain preliminary steps for processing the biological sample and transferring a portion of the processed sample into a reaction vessel containing a bisulfite mixture. The method can further comprise heating the reaction vessel containing the biological sample and the bisulfite mixture at several heating temperatures and subsequently holding the reaction vessel at a holding temperature for a holding period. The method can also comprise certain bisulfite removal steps, desulfonation steps, and removal of the desulfonation solution. A final elution step can yield the converted DNA for further downstream sequencing and analysis.
Type:
Grant
Filed:
October 28, 2021
Date of Patent:
May 17, 2022
Assignee:
URIT Medical Electronic Co., Ltd.
Inventors:
Tom Cheng Xu, Kuikui Shen, Sally Li Shen, Jinlan Xu, Shilei Tang, Jusong Liang
Abstract: The present invention provides novel methods of diagnosing and determining treatment strategies for Lyme disease and other tick-borne illnesses.
Type:
Grant
Filed:
November 18, 2019
Date of Patent:
May 10, 2022
Assignee:
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
Abstract: The present invention relates to a nucleic acid detection kit and a nucleic acid detection method, which use nanoparticles. More specifically, the present invention relates to: a nucleic acid detection method comprising a step of amplifying and labeling nucleic acids, and then capturing the same by using nanoparticles and centrifuging the same; and a nucleic acid detection kit using the method. The present invention is effective since a negative or positive determination for a particular disease can be made, through the nucleic acid detection method not comprising a separation step, in a more rapid, simple, sensitive, and highly reliable manner.
Type:
Grant
Filed:
July 22, 2016
Date of Patent:
April 5, 2022
Assignee:
NATIONAL CANCER CENTER
Inventors:
Sang Hyun Hwang, Ji Hyun Lim, Su Jin Gang, Do Hoon Lee
Abstract: Disclosed is a method for isolating, amplifying, and sequencing infectious agents' nucleic acids from an acellular fraction of a biological fluid using detergents and nucleic acids-digesting enzymes. Also disclosed is a kit-of-parts including detergents and nucleic acids-digesting enzymes for the implementation of the methods described.
Abstract: Methods for imaging are described, including, but not limited to a method comprising: (1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand, and wherein target-specific binding partners of different specificity are linked to different docking strands, (2) optionally removing unbound target-specific binding partners, (3) contacting the sample with antigen-bound imager strands and antigen-specific binding partners linked (directly or indirectly) to optical labels, wherein the antigen-bound imager strands have complementarity to a docking strand, directly or indirectly, and wherein each antigen-specific binding partner is linked to one or more optical labels, and wherein antigen-specific binding partners of different specificity are linked to distinct optical labels, (4) optionally removing unbound antigen-bound imager strands and/or antigen-specific binding partner
Abstract: The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.
Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.
Type:
Grant
Filed:
August 9, 2018
Date of Patent:
March 8, 2022
Assignee:
IMEC VZW
Inventors:
Paolo Fiorini, Tim Stakenborg, Frederik Colle
Abstract: The present invention relates to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, and more particularly to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, which can accurately diagnose the presence or absence of a mutation in exon 4 of BIGH3 gene, which is responsible for Avellino corneal dystrophy. The use of the primer pair and probe according to the invention can diagnose Avellino corneal dystrophy in a more rapid and accurate manner than a conventional method that uses a DNA chip or PCR.
Abstract: Methods and compositions for the amplification of nucleic acids are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases, ligases, and primers.
Abstract: Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform PCR amplification. Embodiments of the invention are also compatible with quantitative Polymerase Chain Reaction (“qPCR”) processes. Microfluidic devices in accordance with the invention may contain a plurality of parallel processing channels. Fully independent reactions can take place in each of the plurality of parallel processing channels. The availability of independent processing channels allows a microfluidic device in accordance with the invention to be used in a number of ways. For example, separate samples could be processed in each of the independent processing channels. Alternatively, different loci on a single sample could be processed in multiple processing channels.
Type:
Grant
Filed:
May 25, 2018
Date of Patent:
February 1, 2022
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Morten J. Jensen, Andrea W. Chow, Colin B. Kennedy, Stephane Mouradian
Abstract: A diagnostic test assembly, including: a substrate having formed therein a plurality of mutually spaced open reservoirs and fluidic channels; a deformable membrane attached to the substrate to cover the open reservoirs and fluidic channels; a rigid covering disposed over the deformable membrane, the rigid covering being configured to allow respective actuators external to the diagnostic test assembly to displace corresponding portions of the deformable membrane; wherein at least some of the portions of the deformable membrane act as pumping portions, each pumping portion being disposed over a corresponding one of the reservoirs and being configured so that when it is displaced by a corresponding actuator, it is displaced into the corresponding reservoir to pump fluid from the corresponding reservoir through at least a corresponding one of the fluidic channels; and wherein one or more of the portions of the deformable membrane act as valve portions, each valve portion being configured so that when it is displa
Abstract: The present disclosure describes systems and devices capable of providing rapid polymerase chain reaction processes. A microfluidic card is insertable into a heating assembly. The heating assembly provides separate temperature zones to the card. The card includes a channel array that traverses repeatedly through the separate temperature zones so that a reaction mixture passing through the channel is subjected to thermal cycling.
Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.
Type:
Grant
Filed:
February 12, 2020
Date of Patent:
December 7, 2021
Assignee:
Exact Sciences Development Company, LLC
Inventors:
Hatim T. Allawi, William G. Weisburg, Graham P. Lidgard, Michael W. Kaiser, Abram M. Vaccaro, Gracie Shea
Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
Type:
Grant
Filed:
December 17, 2018
Date of Patent:
December 7, 2021
Assignee:
Life Technologies Corporation
Inventors:
Mark Andersen, Michael Pallas, Haopeng Wang