Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
Type:
Grant
Filed:
December 17, 2018
Date of Patent:
December 7, 2021
Assignee:
Life Technologies Corporation
Inventors:
Mark Andersen, Michael Pallas, Haopeng Wang
Abstract: Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer.
Abstract: This invention relates to prognostic signatures, and compositions and methods for determining the prognosis of cancer in a patient, particularly for colorectal cancer. Specifically, this invention relates to the use of genetic markers for the prediction of the prognosis of cancer, such as colorectal cancer based on signatures of genetic markers. In various aspects, the invention relates to a method of predicting the likelihood of long-term survival of a cancer patient, a method of determining a treatment regime for a cancer patient, a method of preparing a treatment modality for a cancer patient, among other methods as well as kits and devices for carrying out these methods.
Type:
Grant
Filed:
August 27, 2019
Date of Patent:
November 23, 2021
Assignee:
PACIFIC EDGE LIMITED
Inventors:
Hjalmar Nekarda, Jan Friederichs, Bernhard Holzmann, Robert Rosenberg, Anthony Edmund Reeve, Michael Alan Black, John Lindsay McCall, Yu-Hsin Lin, Robert Craig Pollock
Abstract: A method of nucleic acid amplification involving using a first modified primer which provides protection to the amplification product from exonuclease degradation and a second primer. The method provides a double stranded nucleic acid, one strand of which is degraded by a double strand nucleic acid specific exonuclease to form a single stranded nucleic acid, which is protected from exonuclease degradation.
Type:
Grant
Filed:
July 18, 2014
Date of Patent:
November 23, 2021
Assignee:
Binx Health Limited
Inventors:
Daniel Adlerstein, David M. Pearce, Anna Dixon, Wellington Fakanya
Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.
Type:
Grant
Filed:
July 16, 2018
Date of Patent:
November 16, 2021
Assignees:
University of Washington, Global Life Sciences Solutions Operations UK Ltd.
Inventors:
David Roger Moore, Matthew Jeremiah Misner, Andrew Arthur Paul Burns, Joshua Bishop, Lisa K. Lafleur, Maxwell Wheeler
Abstract: A nucleic acid patch method for amplifying target nucleic acid sequences in circulating free DNA or residual DNA samples where the defining ends of the target nucleic acid sequences are unknown.
Type:
Grant
Filed:
May 2, 2018
Date of Patent:
November 2, 2021
Assignee:
KAILOS GENETICS, INC.
Inventors:
Katherine E. Varley, D. Troy Moore, Randall Bachmeyer, David Kloske
Abstract: Methods for the rapid detection of the presence or absence of Zika virus in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting Zika virus and kits are provided that are designed for the detection of Zika virus.
Type:
Grant
Filed:
April 6, 2020
Date of Patent:
October 26, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Slav Dugenny, Ellen H Fiss, Jeffery Fong, Marintha Heil, Eugene Spier, Jingtao Sun
Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
Type:
Grant
Filed:
February 11, 2020
Date of Patent:
October 12, 2021
Assignee:
HandyLab, Inc.
Inventors:
Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
Abstract: The invention features a fully-integrated rapid molecular diagnostic device that is low-cost, easy to manufacture, and simple to use. The device can serve as a molecular diagnostic platform for any disease, requiring little or no preparation or customization and can be made from simple materials (e.g., paper and adhesive film), making it inexpensive, portable, and disposable. The invention also provides methods of using the device for detection of one or more targets in a sample.
Type:
Grant
Filed:
December 12, 2016
Date of Patent:
October 5, 2021
Assignee:
Trustees of Boston University
Inventors:
Catherine M. Klapperich, Natalia M. Rodriguez, Jacqueline C. Linnes
Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.
Abstract: A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.
Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.
Abstract: Provided for herein is a method for detecting the presence of a nucleic acid target molecule in a biological sample. In certain aspects, the method comprises contacting a test sample that comprises (i) a biological sample comprising a nucleic acid target molecule and (ii) an osmylated single-stranded oligonucleotide probe comprising at least one pyrimidine residue covalently bonded to a substituted or unsubstituted Osmium tetroxide (OsO4)-2,2?-bypyridine group (OsBp group).
Abstract: Described herein are methods and means for reliably releasing maternal and/or paternal DNA from single seeds without substantially affecting the subsequent germination of the seeds. The methods are particularly suited for genome analysis of small grain cereals, including wheat. The methods may include enzymatic treatments for improved DNA recovery.
Type:
Grant
Filed:
March 29, 2017
Date of Patent:
August 31, 2021
Assignee:
BASF SE
Inventors:
Kristof Van der Meeren, Rainhard Koch, Philipp Ellinger, Nina Jansen, Stephen Rae, Martin Clausen
Abstract: A composition comprising a thermostable DNA polymerase; and a PCR inhibitor blocking agent, wherein the PCR inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled PCR to a PCR inhibitor.
Type:
Grant
Filed:
June 30, 2016
Date of Patent:
August 17, 2021
Assignee:
Life Technologies Corporation
Inventors:
Christopher Trinh, Tom Xu, Yating Shi, Ferrier Le, Claire Marjoribanks
Abstract: The present invention relates to a low-cost, thermally reversible valve for paper-fluidic diagnostic devices. In particular, this invention demonstrates a tunable valve mechanism fabricated by wax-ink printing and localized heating via thin-film resistors to sequentially release liquids through a cellulose or nitrocellulose membrane. The wax-ink valve can obstruct fluid flow for a sustained time and are thermally actuated to release a controlled amount of liquid past the valve. This integrated paper-fluidic diagnostic assay device requires minimal user involvement, can be easily manufactured and tuned to meet various fluid delivery timing and incubation needs.
Type:
Grant
Filed:
April 19, 2017
Date of Patent:
August 17, 2021
Assignee:
Purdue Research Foundation
Inventors:
Jacqueline C Linnes, Megan Zaiyi Chiu, Rui Shen
Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
Type:
Grant
Filed:
January 11, 2021
Date of Patent:
August 10, 2021
Assignee:
HandyLab, Inc.
Inventors:
Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
Abstract: Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5? sequence flanking the 5? end of the internal sequence and a 3? flanking sequence flanking the 3? end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.
Type:
Grant
Filed:
December 13, 2019
Date of Patent:
July 27, 2021
Assignee:
Gen9, Inc.
Inventors:
Michael E. Hudson, Li-Yun A. Kung, Daniel Schindler, Stephen Archer, Ishtiaq Saaem