Abstract: Circular RNA and methods and constructs for engineering circular RNA are disclosed. In some embodiments, the circular RNA includes the following elements arranged in the following sequence: a) a 3? Group I self-splicing intron fragment, b) an internal ribosome entry site (IRES), c) a protein coding region or noncoding region, and d) a 5? Group I self-splicing intron fragment.

Abstract: Disclosed are methods and constructs for engineering circular RNA Disclosed is a vector for making circular RNA, said vector comprising the following elements operably connected to each other and arranged in the following sequence: a) a 5? homology arm, b) a 3? group I intron fragment containing a 3? splice site dinucleotide, c) an optional 5? spacer sequence, d) a protein coding or noncoding region, e) an optional 3? spacer sequence, f) a 5? Group I intron fragment containing a 5? splice site dinucleotide, and g) a 3? homology arm. This vector allows production of a circular RNA that is translatable or biologically active inside eukaryotic cells. In one embodiment, the vector can comprise the 5? spacer sequence, but not the 3? spacer sequence. In yet another embodiment, the vector can also comprise the 3? spacer sequence, but not the 5? spacer sequence.

Abstract: A method and system for predicting process information (e.g., phase data) using a given input (e.g., intensity) to a parameterized model are described. A latent space of a given input is determined based on dimensional data in a latent space of the parameterized model for a given input to the parameterized model. Further, an optimum latent space is determined by constraining the latent space with prior information (e.g., wavelength) that enables converging to a solution that causes more accurate predictions of the process information. The optimum latent space is used to predict the process information. The given input may be a measured amplitude (e.g., intensity) associated with the complex electric field image. The predicted process information can be complex electric field image having amplitude data and phase data. The parameterized model comprises variational encoder-decoder architecture.

Abstract: Disclosed herein are compounds of formula I: or a pharmaceutically acceptable salt thereof, where the variables are as defined herein. These compounds are useful in treating RET associated cancers. Formulations containing the compounds of formula I and methods of making the compounds of formula I are also disclosed.

Abstract: In a lithographic process, product units such as semiconductor wafers are subjected to lithographic patterning operations and chemical and physical processing operations. Alignment data or other measurements are made at stages during the performance of the process to obtain object data representing positional deviation or other parameters measured at points spatially distributed across each unit. This object data is used to obtain diagnostic information by performing a multivariate analysis to decompose a set of vectors representing the units in the multidimensional space into one or more component vectors. Diagnostic information about the industrial process is extracted using the component vectors. The performance of the industrial process for subsequent product units can be controlled based on the extracted diagnostic information.

Abstract: A novel gRNA-ligand binding complex is provided. This complex may be used to bring Type V Cas proteins and additional effectors to DNA for base editing. The design of the systems allows for the production of efficient modular components that provide flexibility when editing DNA.

Abstract: A method of equalising an audio signal derived from a microphone, the method comprising: receiving the audio signal; applying an order-statistic filter to the audio signal in the frequency domain to generate a statistically filtered audio signal; equalising the received audio signal based on the statistically filtered audio signal to generate an equalised audio signal.

Abstract: In one aspect the invention provides a sensing apparatus comprising a set of N capacitive sensors connected over a single physical channel provided by two terminals, the apparatus comprising: a sensing array having a first capacitive sensor and N?1 parallel capacitive sensors each connected in parallel with first capacitive sensor, each capacitive sensor having electrodes separated by a dielectric to provide a capacitance which is able to vary with deformation to provide sensing, the array having a set of N?1 resistances each being in series with a respective one of the N?1 parallel capacitive sensors; a solution module operable to determine the capacitance of each capacitive sensor by finding solutions for a vector function equating reactance measured at the two terminals to an analytical model for impedance and/or reactance of the circuit seen at the two terminals, wherein the analytical model comprises N capacitances, each connected in parallel with each other, and comprises N series resistances each conne

Abstract: A method (2800) of selectively leaching one or more manganese-containing phases from a mixed-phase battery electrode material comprises treating (2802) the mixed-phase battery electrode material with a solution of an acid, the acid acting as both a leaching agent and a reducing agent, so as to form a manganese-containing leachate whilst leaving at least one phase of the battery electrode material unleached, wherein the acid has a pKa greater than or equal to ?2. Either or both of the leachate and the remaining electrode material may then be regenerated (2806, 2808).

Abstract: There is disclosed a system and method for forecasting the positions of marine vessels. In an aspect, the present system is adapted to execute a forecasting algorithm to forecast the positions of one or a great many marine vessel(s) based on one or more position reporting systems including coastal and satellite AIS (S-AIS) signals or LRIT received from the vessel. The forecasting algorithm utilizes location and direction information for the vessel, and estimates one or more possible positions based on previous paths taken by vessels from that location, and heading in substantially the same direction. Thus, a body of water can be divided into “bins” of location and direction information, and a spatial index can be built based on the previous paths taken by other vessels after passing through that bin. Other types of information may also be taken into account, such as ship-specific data, nearby weather, ocean currents, the time of year, and other spatial variables specific to that bin.

Abstract: The disclosure is directed at a system and method for rapid detection of low level bacteria in a growth medium. The method is directed at a two-stage process whereby a growth medium is incubated along with a first test formulation component for a predetermined period of time. After the incubation period, a second test formulation component is added to the growth medium to determine if there is a bacteria in the growth medium.

Abstract: A structured-light-velocimetry method includes extracting one or more bursts from a time-varying signal generated by detecting scattered light from a tracer particle passing through a structured optical beam; fitting each of the one or more bursts to a multi-variable model to extract a plurality of fitted parameters; and executing a machine-learning model with the plurality of fitted parameters to predict an angular velocity of the tracer particle.

Abstract: The disclosure is directed at a system and method for non-microbial prediction of antimicrobial efficacy of an antimicrobial item, such as, a metal surface or materials impregnated with biocidal metal ions or nanoparticles. A test formulation is applied to a surface of interest and a color change of the test formulation is then analyzed and then correlated with antimicrobial activity.

Abstract: A flexible switch has a deformable body and a plurality of electrodes, at least one of the electrodes being provided on the deformable body. The switch has a first state in which the electrodes are spaced apart and a second state in which the electrodes are in electrical contact, and the switch is configured to allow movement between the states when a force is applied to or removed from the deformable body.

Abstract: A vehicle includes a first controller that sends a request to enter a lower power mode, and a plurality of second controllers. The second controllers each periodically send the first controller signals indicating operation in a normal power mode while being powered by a primary power converter, and responsive to receiving the request, do not send the signals during the low power mode while being capable of powered by an auxiliary power converter that has a maximum power limit less than the primary power converter. Also, the first controller, responsive to not receiving the signals for a predetermined period of time, disconnects the first and second controllers from the primary power converter.

Abstract: A method of equalising an audio signal derived from a microphone, the method comprising: receiving the audio signal; applying an order-statistic filter to the audio signal in the frequency domain to generate a statistically filtered audio signal; equalising the received audio signal based on the statistically filtered audio signal to generate an equalised audio signal.

Abstract: A flexible switch has a deformable body and a plurality of electrodes, at least one of the electrodes being provided on the deformable body. The switch has a first state in which the electrodes are spaced apart and a second state in which the electrodes are in electrical contact, and the switch is configured to allow movement between the states when a force is applied to or removed from the deformable body.

Abstract: A method of equalising an audio signal derived from a microphone, the method comprising: receiving the audio signal; applying an order-statistic filter to the audio signal in the frequency domain to generate a statistically filtered audio signal; equalising the received audio signal based on the statistically filtered audio signal to generate an equalised audio signal.

Abstract: Systems and methods for slide image alignment are described herein. An example method includes receiving a plurality of slide images, detecting a plurality of features contained in the slide images, and comparing a plurality of pairs of the slide images. The comparison uses the detected features. The method also includes creating a distance matrix that reflects a respective difference between each of the pairs of the slide images, creating a graph by connecting each of the slide images to its most similar slide image, and detecting a plurality of graph components. Each of the graph components includes one or more of the slide images. The method further includes aligning the slide images within each of the graph components, and aligning the graph components to form a composite image.

Abstract: A vehicle includes a first controller that sends a request to enter a lower power mode, and a plurality of second controllers. The second controllers each periodically send the first controller signals indicating operation in a normal power mode while being powered by a primary power converter, and responsive to receiving the request, do not send the signals during the low power mode while being capable of powered by an auxiliary power converter that has a maximum power limit less than the primary power converter. Also, the first controller, responsive to not receiving the signals for a predetermined period of time, disconnects the first and second controllers from the primary power converter.