Abstract: Currently, there is interest in applying machine learning techniques to analyse digital pathology images automatically. Machine learning techniques often rely on training with a ground-truth image input. The quality and amount of training data determines the quality of the detector, as expressed in the rate of true and false positives, and robustness against variations in the appearance of the input images. The present application proposes to obtain image data of the same sample before and after at least one re-staining step (firstly with a structure-revealing stain, and secondly with a bio marker revealing stain). Sections of the first and second image data having a good registration relationship are chosen, along with the probability of detecting a desired candidate object (such as nucleus) and the probability of the bio marker revealing stain being present annotation data suitable for training a machine learning algorithm on the first and/or the second image data is provided.

Abstract: The invention relates to a printing control device (10) to control printing of a cover layer on a tissue or cell sample to be examined, a system (1) for printing of a cover layer (1) on a tissue or cell sample to be examined, a method to control printing of a cover layer on a tissue or cell sample to be sample to be examined, a computer program element for controlling such device or system for performing such method and a computer readable medium having stored such computer program element. The printing control device (10) comprises an imaging unit (11) and a printing control unit (12). The imaging unit (11) is configured to provide image data of the sample, and to determine a local image parameter from the image data. The local image parameter relates to local tissue porosity and/or a local capillary force of the sample. The printing control unit (12) is configured to control a printing parameter for printing the cover layer on the sample based on the local image parameter.

Abstract: Disclosed is a device for controlling a volume of an analysis chamber of a micro chamber arrangement to which a region of interest of an object is exposed. The volume is controlled using a volume reducing element which is deposited on a surface of the micro chamber arrangement. The device comprises a deposition unit configured to determine a position and an extent of the volume reducing element depending on (a) the region of interest and further depending on (b) a predetermined level by which a volume of the analysis chamber is reduced using the volume reducing structure. The deposition unit is further configured to deposit the volume reducing element depending on the determined position and extent.

Abstract: The present invention relates to an illustration of an annotation (22) at a sample slide (14). It is the intention for the invention to copy the annotation (22) provided at a reference slide (12) to the sample slide (14). Typically, a reference image (26) of the reference slide (12) is provided, in particular by scanning the reference slide (12). The reference image (26) therefore has the information about a reference slice (16) which is carried by the reference slide (12). Furthermore, the reference slide (12) has been marked with a region of interest (20) and the annotation (22), which can be associated with the region of interest (20). If such reference slide (12) is scanned, the respective reference image (26) comprises the information about the marked region of interest (20) as well as the annotation (22), too. Furthermore, the sample slide (14) carrying a sample slice (18) can be scanned, in order to provide a sample image (28).

Abstract: The present invention relates to detecting objects in medical images. In order to provide an improved detection of objects in medical images, a medical image detection device (10) is provided that comprises an image data input (12) and a processing unit (14). The image data input is configured to receive image data of a biological sample. The processing unit comprises a detector (16) and a classifier (18). The detector is configured to detect objects of interest in the sample by a detection in the image data of at least one predetermined object feature. The detected objects being candidate objects, wherein the candidate objects comprise true positives and possible false positives. Further, the classifier is configured to classify the possible false positives as false positives or as true positives. The classifier is a trained classifier, trained specifically to recognize the false positives of the detector.

Abstract: A method is presented that enables the spatial mapping of nucleic acids of tissue samples with high resolution and without sacrificing the degree of multiplexing that is available from next-generation sequencing. The method is based on the application of patterns of barcoded oligonucleotides probes onto predefined locations in a region of interest in a tissue sample. Every nucleic acid analyzed can be allocated to a certain position inside the sample based on the barcode. Various printing technologies can be used and different ways of patterning can be employed, like a regular array with a certain pitch or alternatively an object-based patterning with defined regions of interest without shape constraints.

Abstract: A real-time metocean sensor array system may include a one or more floating instruments each including geolocation capabilities and connected to a satellite communication network. In some examples, the floating instruments may further include an omnidirectional hydrophone. Motion and acoustical data gathered by the instruments may be converted by onboard processing logic into wave, current, and/or wind-related observations that may be communicated in real time and analyzed via a cloud-based system.

Abstract: The present invention relates to digital pathology. In order to facilitate analyzing a tissue microarray, an apparatus is provided for tissue examination. The apparatus comprises a data input (102), a tissue microarray analyzing unit (104), and an output (106). The data input is configured to receive a reference image of a reference slice obtained from a tissue sample block; and to receive a microarray image of a microarray slice comprising at least one tissue core obtained from at least the tissue sample block. The tissue microarray analyzing unit is configured to register tissue core images of at least one tissue core with the reference image based on a spatial arrangement of the respective tissue core within the tissue sample block. The output is configured to provide a registered result obtained from the tissue microarray analyzing unit for further analyzing purposes.

Abstract: The present invention relates to digital pathology. In order to improve the workflow in the process of selecting a region of interest of an unstained sample to be removed for molecular diagnostic, a method (100) is provided for selecting a sample removing area of an unstained sample to be removed for molecular diagnostic. The method comprises the following steps: In a first step 102, also referred to as step a), a reference removing area is selected in a reference image of a reference slice of an object, wherein biological material in the reference slice is stained. In a second step 104, also referred to as step b), a digital sample image of a sample slice of the object is obtained under an imaging setting. The biological material in the sample slice is unstained. The sample slice is received on a sample slide and positioned in an optical path between a light source and an image detector.

Abstract: A device (100) and a method optically control a chemical reaction in a reaction chamber (149) holding a reagent fluid (114). The chemical reaction includes a nucleic acid sequencing on a wiregrid. Based on strong optical confinement of excitation light (110) and of cleavage light (112), the sequencing reaction can be read-out. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked. In order to avoid overheating by cleavage light, the reagent fluid is circulated along the surface of the substrate (101).

Abstract: The invention relates to a fluidic system comprising at least one bead chamber (311) containing a lyophilized reagent (LB) and a reaction chamber in a cartridge. In one embodiment, a series of bead chambers with different lyophilized reagents may be provided such that sample fluid can sequentially pass through them. In another embodiment, bead chambers may be located on a movable carrier, for example a rotating carousel, from which they may selectively be connected to a reaction chamber in a cartridge. In still another embodiment, the bead chamber (311) may comprise at least one flexible wall (FW) allowing for a minimization of dead volume associated with the extraction of lyophilized reagent (LB).

Abstract: A real-time metocean sensor array system may include a one or more floating instruments each including geolocation capabilities and connected to a satellite communication network. In some examples, the floating instruments may further include an omnidirectional hydrophone. Motion and acoustical data gathered by the instruments may be converted by onboard processing logic into wave, current, and/or wind-related observations that may be communicated in real time and analyzed via a cloud-based system.

Abstract: A method of aligning a template and a substrate for imprint lithography involves using a mask pattern of the template and a luminescent marker pattern of the substrate, the method including aligning the template mask pattern and the substrate marker pattern using a radiation intensity measurement of radiation emitted by the luminescent marker pattern and having passed the template mask pattern. The mask pattern and the luminescent marker pattern may each be shaped to provide a turning point in the intensity of detected radiation emitted from the marker pattern, and passing through the mask pattern to a detector, as a function of relative displacement at the aligned position. The displacement of the template and substrate may be aligned by identifying the turning point in radiation intensity. The marker pattern may be fluorescent with the emitted radiation excited by a radiation source.

Abstract: A real-time metocean sensor array system may include a one or more floating instruments each including geolocation capabilities and connected to a satellite communication network. In some examples, the floating instruments may further include an omnidirectional hydrophone. Motion and acoustical data gathered by the instruments may be converted by onboard processing logic into wave, current, and/or wind-related observations that may be communicated in real time and analyzed via a cloud-based system.

Abstract: The present invention relates to digital pathology. In order to improve the workflow in the process of selecting a region of interest of an unstained sample to be removed for molecular diagnostic, a method (100) is provided for selecting a sample removing area of an unstained sample to be removed for molecular diagnostic. The method comprises the following steps: In a first step 102, also referred to as step a), a reference removing area is selected in a reference image of a reference slice of an object, wherein biological material in the reference slice is stained. In a second step 104, also referred to as step b), a digital sample image of a sample slice of the object is obtained under an imaging setting. The biological material in the sample slice is unstained. The sample slice is received on a sample slide and positioned in an optical path between a light source and an image detector.

Abstract: The present invention relates to digital pathology. In order to facilitate analyzing a tissue microarray, an apparatus is provided for tissue examination. The apparatus comprises a data input (102), a tissue microarray analyzing unit (104), and an output (106). The data input is configured to receive a reference image of a reference slice obtained from a tissue sample block; and to receive a microarray image of a microarray slice comprising at least one tissue core obtained from at least the tissue sample block. The tissue microarray analyzing unit is configured to register tissue core images of at least one tissue core with the reference image based on a spatial arrangement of the respective tissue core within the tissue sample block. The output is configured to provide a registered result obtained from the tissue microarray analyzing unit for further analyzing purposes.

Abstract: The present invention relates to an illustration of an annotation (22) at a sample slide (14). It is the intention for the invention to copy the annotation (22) provided at a reference slide (12) to the sample slide (14). Typically, a reference image (26) of the reference slide (12) is provided, in particular by scanning the reference slide (12). The reference image (26) therefore has the information about a reference slice (16) which is carried by the reference slide (12). Furthermore, the reference slide (12) has been marked with a region of interest (20) and the annotation (22), which can be associated with the region of interest (20). If such reference slide (12) is scanned, the respective reference image (26) comprises the information about the marked region of interest (20) as well as the annotation (22), too. Furthermore, the sample slide (14) carrying a sample slice (18) can be scanned, in order to provide a sample image (28).

Abstract: A medicament delivery device, which comprises a driving mechanism and a housing (1) having a reservoir (2), which is at least partly filled with a substance and which is sealed at least on one side with a flexible wall (21). The driving mechanism is adapted to press a piston (23) against the flexible wall (21) thereby releasing the substance from the reservoir (2) through an opening (25) in the reservoir (2). This provides for a safe and reliable medicament delivery device.

Abstract: A method is presented that enables the spatial mapping of nucleic acids of tissue samples with high resolution and without sacrificing the degree of multiplexing that is available from next-generation sequencing. The method is based on the application of patterns of barcoded oligonucleotides probes onto predefined locations in a region of interest in a tissue sample. Every nucleic acid analyzed can be allocated to a certain position inside the sample based on the barcode. Various printing technologies can be used and different ways of patterning can be employed, like a regular array with a certain pitch or alternatively an object-based patterning with defined regions of interest without shape constraints.