Abstract: A system and method are disclosed for pairing computing devices using an authentication protocol that allows an initiating computing device to gain access to a secure, encrypted network of a target computing device.

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

Abstract: Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

Abstract: A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a TCR or a TCR-binding antigen. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

Abstract: Provided is a shoe that includes: a bottom surface that is adjacent to the ground in normal use; a sole that forms at least a portion of the bottom surface; an upper portion extending above the sole; and individual particles bonded to the bottom surface of the shoe. The individual particles include at least one of corn husk, hemp or a natural plant material that has been ground into the individual particles.

Abstract: Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

Abstract: Provided is a shoe that includes: a bottom surface that is adjacent to the ground in normal use; a sole that forms at least a portion of the bottom surface; an upper portion extending above the sole; and individual particles bonded to the bottom surface of the shoe. The individual particles include at least one of corn husk, hemp or a natural plant material that has been ground into the individual particles.

Abstract: A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

Abstract: A method of playing a sporting event interactive board game is provided. A sporting event is displayed on at least one of a television, a computer monitor or other electronic device. The present invention includes a game board. The game board includes a playing surface having a plurality of betting spaces forming a path. Each player may purchase at least one of the betting spaces to play the game. The present invention further includes a chip. A position of the chip is determined randomly and placed on one of the betting spaces. The chip is then moved along the path of the betting spaces based on certain plays performed during the sporting event. When a score or an end of a period occurs during the sporting event, the player who purchased the betting space in which the chip is currently resting is paid.

Abstract: A football board game for playing a game board version of football between opposing players is provided. The game comprises a game table having a top surface, a bottom surface, and a side wall around the complete perimeter of the game table. First and second legs are secured to the bottom surface of the game table with the legs extendable to support the game table above a surface and retractable to a position against the bottom surface of the game table. A carpeted football field is mounted to the top surface of the game table and configured to represent a standard football field and marked appropriately with yard lines, goal lines, and yardage. Two field goalposts are positionable on opposite ends of the football field. Also included are a set of thirty-two NFL cards, a pair of dice, two team figure helmets, and two yellow time out cards.

Abstract: An interlock assembly for a door latch mechanism of a door in a container for bulk material that has an interior lined with a membrane for holding fluid. The interlock assembly includes a plunger in an aperture formed through the door, which moves between an extended position, where the plunger extends into the interior of the container, and a locked position, where the plunger is urged outwardly from of the interior of the container. A spring is positioned to apply a force to urge the plunger to the extended position. A lock member is connected to move with the plunger between the extended position and the locked position. The spring force is selected to enable the membrane, under static pressure of fluid within the container, to urge the plunger to the locked position, thereby engaging the lock member to interlock against operation of the door latch.

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

Abstract: A printing screen for printing deposits of a print medium onto workpieces, the printing screen comprising a sheet in which a pattern of printing apertures are formed to allow for printing of a pattern of deposits onto a workpiece, and a waveguide which can be driven to induce an ultrasonic vibration in the sheet, wherein the waveguide comprises an elongate body at a surface of the sheet which at least partially surrounds the pattern of printing apertures.

Abstract: A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

Abstract: A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

Abstract: A method of playing a sporting event interactive board game is provided. A sporting event is displayed on at least one of a television, a computer monitor or other electronic device. The present invention includes a game board. The game board includes a playing surface having a plurality of betting spaces forming a path. Each player may purchase at least one of the betting spaces to play the game. The present invention further includes a chip. A position of the chip is determined randomly and placed on one of the betting spaces. The chip is then moved along the path of the betting spaces based on certain plays performed during the sporting event. When a score or an end of a period occurs during the sporting event, the player who purchased the betting space in which the chip is currently resting is paid.

Abstract: Provided is a shoe that includes: a bottom surface that is adjacent to the ground in normal use; a sole that forms at least a portion of the bottom surface; an upper portion extending above the sole; and individual particles bonded to the bottom surface of the shoe. The individual particles include at least one of corn husk, hemp or a natural plant material that has been ground into the individual particles.