Abstract: Disclosed are improved aqueous processes and compositions for obtaining a "stonewashed", look in colored fabric while reducing the amount of redeposition of colorant onto the fabric, as well as the fabrics produced from these methods. In particular, the disclosed methods as directed to contacting fabrics with fungal cellulase composition which is substantially free of CBH type components. Fabrics so treated show reduced redeposition of colorant.

Abstract: A method for preparing an aqueous solution enriched in EG III from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The method involves adding an amount of a low molecular weight alcohol selected from the group consisting of ethanol, methanol, propanol and mixtures thereof to the aqueous mixture containing cellulase proteins, xylanase and EG III and an organic salt under conditions wherein substantially all of the cellulase proteins other than EG III and xylanase are precipitated out of the aqueous mixture. The method then involves removing the precipitate from the aqueous mixture so as to recover an aqueous supernate enriched in EG III. Next, the method involves adding an amount of an inorganic salt to the supernate produced in step b) so as to form a second precipitate and a second supernate and then finally collecting the second supernate from the second precipitate to obtain a supernate enriched in EG III.

Abstract: A method for preparing an aqueous solution enriched in xylanase only, EG III only and both EG III and xylanase from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The methods involve adding an amount of a low molecular weight alcohol and an organic salt to an aqueous mixture containing cellulase proteins under conditions wherein substantially all of the cellulase proteins other than EG III and xylanase are precipitated out of the aqueous mixture. The methods can then involve adding an inorganic salt to the supernate produced in the previous step so as to form a second precipitate and a second supernate and then finally collecting either the second precipitate from the second supernate to obtain a precipitate enriched in xylanase or the second supernate from the second precipitate to obtain a supernate enriched in EG III.

Abstract: Methods for isolating EG III cellulase component and enriched EG III cellulase in polyethylene glycol are disclosed. The methods comprise adding an inorganic salt and polyethylene glycol having a molecular weight of from about 5,000 to 10,000 to an aqueous mixture of cellulase proteins under conditions to create a two-phase system. Next, the cellulase proteins other than EG III are separated in an EG III-poor aqueous phase while EG III cellulase component is retained in an EG III-rich polyethylene glycol phase. Lastly, the EG III component can be separated from the polyethylene glycol. The preferred method of separation is alcohol precipitation.

Abstract: A method for preparing an aqueous solution enriched in xylanase from an aqueous mixture containing cellulase proteins and xylanase is disclosed. The method involves adding an amount of a low molecular weight alcohol to an aqueous mixture containing cellulase proteins and an organic salt so as to precipitate out the cellulase proteins other than EG III from the mixture. The method then involves adding an inorganic salt to the supernate produced in the previous step so as to form a second precipitate and a second supernate and then finally collecting the second precipitate from the second supernate to obtain a precipitate enriched in xylanase.