Abstract: The invention provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit comprising at least two mutations, for example double and triple mutants, which are useful for producing transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides expression vectors, cells, plants comprising the polynucleotides encoding the AHAS large subunit double and triple mutants, plants comprising two or more AHAS large subunit single mutant polypeptides, and methods for making and using the same.

Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant by means of Agrobacterium-mediated transformation. Preferred is the use of the Zea may lines deposited with American Type Culture Collection under the Patent Deposit Designation PTA-6170 and PTA-6171.

Abstract: The present invention is directed to nucleic acids encoding polypeptides that confer upon a plant tolerance to an imidazolinone and/or other acetohydroxyacid synthase (AHAS) inhibiting herbicide when expressed in the plant. The present invention also provides plants having increased tolerance to an imidazolinone and/or other AHAS-inhibiting herbicide. More particularly, the present invention includes plants containing at least one IMI nucleic acid. The present invention also includes seeds produced by these plants and methods of controlling weeds in the vicinity of these wheat plants.

Abstract: Isolated polynucleotides that encode acetohydroxyacid synthase small subunit (AHASS) polypeptides, and the amino acid sequences encoding these polypeptides, are described. Expression cassettes and expression vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, expression cassettes, and expression vectors, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides are also described.

Abstract: The present invention is directed to wheat plants and triticale plants having increased tolerance to an imidazolinone herbicide. More particularly, the present invention includes wheat plants or triticale plants containing one or more Triticum turgidum IMI nucleic acids. The present invention also includes seeds produced by these wheat plants and triticale plants, and methods of controlling weeds in the vicinity of these wheat plants and triticale plants.

Abstract: The present invention is directed to plants having increased tolerance to an imidazolinone herbicide. More particularly, the present invention includes rice plants containing at least one variant AHAS nucleic acid such as an imidazolinone tolerant IMINTA 1, 4 or 5 lines comprising an alanine to threonine substitution as compared to the wild-type AHAS. The present invention also includes seeds produced by these rice plants and methods of controlling weeds in the vicinity of these rice plants.

Abstract: Isolated polynucleotide molecules that encode mature, wild-type and imidazolinone-tolerant acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences encoding these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotide molecules of the invention, as well as plants and host cells transformed with the polynucleotide molecules, expression cassettes, and transformation vectors, are described. Methods of using the polynucleotide molecules to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of such plants are also described.

Abstract: The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

Abstract: The present invention provides purified isolated nucleic acids encoding arabidopsis 4-hydroxyphenylpyruvate dioxygenase (HPPD), plasmids encoding HPPD, and transformed cells capable of expressing HPPD. The invention also provides methods for identifying HPPD inhibitors.