Abstract: Provided is a method of manufacturing a zinc-plated steel sheet. The method includes: coating a metal on the steel sheet on a steel sheet; annealing the metal coated steel sheet; and zinc plating the annealed steel sheet by dipping in a molten zinc plating bath. Further provided is a method of manufacturing a hot-press part including: coating a metal on the steel sheet on a steel sheet; annealing the metal coated steel sheet; zinc plating the annealed steel sheet by dipping in a molten zinc plating bath; heating the zinc-plated steel sheet; and press forming the heated steel sheet.

Abstract: Disclosed are organelles-specific two-photon fluorescent probes, and more particularly, a blue emission two-photon fluorescent probe capable of selectively detecting calcium ions (Ca2+) through fluorescence signals by targeting the cytoplasm, mitochondria, and plasma membrane even among the organelles, respectively, and a green emission two-photon fluorescent probe capable of selectively detecting hydrogen ions (H+) through fluorescence signals by targeting the lysosome, a method for preparing these two-photon probes, and a method for separately or simultaneously imaging calcium ions in the cytoplasm, mitochondria, or plasma membrane and hydrogen ions in the lysosome using the two two-photon probes.

Abstract: The present disclosure relates to a two-photon probe for in-vivo imaging, having high selectivity for copper(II) ion, and a method for quantitatively estimating copper(II) on (Cu2+) in vivo using the same. The two-photon probe according to the present disclosure can detect intracellular free copper(II) on for a long period of time with a penetration depth greater than 90 ?m in living cells and tissues without the problems of mistargeting and photobleaching and can quantitatively estimate copper(II) on concentration in vivo using the ratio of blue emission intensity at 400-450 nm and red emission intensity at 550-650 nm.

Abstract: The present disclosure relates to a two-photon fluorescent dye for imaging total sulfide in vivo and a method for quantitatively estimating total sulfide concentration in vivo using the same. The two-photon fluorescent dye, which includes a compound of [Chemical Formula 1], can detect hydrosulfide ion and image total sulfide and allows quantitative estimation of total sulfide concentration in vivo.

Abstract: The present disclosure relates to a two-photon probe for in-vivo imaging, having high selectivity for copper(II) ion, and a method for quantitatively estimating copper(II) on (Cu2+) in vivo using the same. The two-phoion probe according to the present disclosure can detect intracellular free copper(H) on for a long period of time with a penetration depth greater than 90 ?m in living cells and tissues without the problems of mistargeting and photobleaching and can quantitatively estimate copper(II) on concentration in vivo using the ratio of blue emission intensity at 400-450 nm and red emission intensity at 550-650 nm.

Abstract: The present disclosure relates to a two-photon fluorescent dye for imaging total sulfide in vivo and a method for quantitatively estimating total sulfide concentration in vivo using the same. The two-photon fluorescent dye, which includes a compound of [Chemical Formula 1], can detect hydrosulfide on and image total sulfide and allows quantitative estimation of total sulfide concentration in vivo.

Abstract: Disclosed herein are compounds useful as two-photon tracers. Also, methods are provided for visualizing intracellular glucose uptake, screening anticancer agents, and diagnosing cancer using the compounds. They exhibit preferential uptake by cancer cells, penetrability sufficient to allow bright section images, high water solubility, high pH resistance and low toxicity in addition to applicability to living cells in deep tissues over a long period of time.

Abstract: Provided are a method for dual-color imaging of sodium/calcium activities using a two-photon fluorescent probe and a method for preparing the two-photon fluorescent probe. The disclosed two-photon fluorescent probe for detecting calcium ions near the cell membrane reacts with calcium cations to exhibit strong two-photon fluorescence and may be selectively and easily loaded into the cell membrane by forming a complex with a calcium ion. Further, it allows imaging of the distribution of calcium cations in a living cell or tissue since it can selectively detect calcium ions in the living cell or tissue at a depth of 100 to 200 ?m for more than 60 minutes. In addition, by staining the living cell or tissue with two probes of different fluorescent colors, the calcium and sodium activities can be imaged simultaneously at different channels.

Abstract: The present disclosure relates to a copper (I) ion-selective fluorescent probe, a method for preparing the same, and a method for diagnosing malignant disease and a diagnosis kit using the probe. The fluorescent probe according to the present disclosure is capable of detecting free copper (I) ions inside cells for a long time with high selectivity and sensitivity for copper (I) ion, with a penetration depth longer than 90 ?m in living cells and tissues and without the problems of mistargeting and photobleaching. Accordingly, since a biological sample can be imaged for a long period of time with high resolution without damage, presence of malignance disease in the target biological sample can be diagnosed faster, more accurately and more easily.

Abstract: A thin film transistor (TFT) liquid crystal display panel and fabrication method are described. The panel has a data line and a gate line connected with a TFT and formed on the same layer. One of data or gate lines is discontinuous and the other is continuous in a pixel region such that the continuous line bisects the discontinuous line. A passivation film protects the TFT. Contact holes penetrate the passivation film and expose segments of the discontinuous line. A contact electrode connects the segments through the contact holes.

Abstract: An organic electroluminescent device is provided. A gate line and a data line cross each other to define a pixel region, and a power line crosses the gate line and is arranged parallel to the data line. A switching transistor is disposed at a crossing of the gate line and the data line, a driving transistor is disposed at a crossing of the gate line and the power line, and a pixel electrode is formed in the pixel region. Each of the switching transistor and the driving transistor includes a gate electrode formed under a channel layer, and a stopper on the channel layer.

Abstract: A two-photon probe for real-time monitoring of intracellular free zinc ions is provided. The two-photon probe is represented by Formula 1: wherein R is H or OCH3. The two-photon probe has high selectivity for Zn2+ and enables very effective and long-term monitoring of intracellular free Zn2+ present in the deep tissue. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular free zinc ions using the two-photon probe.

Abstract: A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20˜50-fold TPEF enhancement in response to Ca2+, has a dissociation constant (KdTP) of 0.14±0.02 to 0.25±0.03 ?M, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 ?m in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe.

Abstract: Provided are two-photon fluorescent probes for imaging acidic vesicles in live cells and tissue. The probes are represented by The probes can selectively bind to vesicles in cytosol to emit two-photon excited fluorescence with high intensity. Therefore, the use of the probes enables effective imaging of acidic vesicles. Further provided is a method for imaging acidic vesicles in live cells and tissue using the probes.

Abstract: Disclosed herein are compounds useful as two-photon tracers. Also, methods are provided for visualizing intracellular glucose uptake, screening anticancer agents, and diagnosing cancer using the compounds. They exhibit preferential uptake by cancer cells, penetrability sufficient to allow bright section images, high water solubility, high pH resistance and low toxicity in addition to applicability to living cells in deep tissues over a long period of time.

Abstract: A two-photon probe for real-time monitoring of intracellular magnesium ions is provided. Specifically, the two-photon probe is represented by Formula 1: wherein R is H or CH2OCOCH3. The two-photon probe is very suitable for real-time imaging of intracellular magnesium ions. The two-photon probe shows 17-fold two-photon excited fluorescence enhancement in response to Mg2+, which is 7-fold stronger than commercial probes, thus enabling staining of cells in a greatly reduced amount. In addition, the two-photon probe has a sufficiently low molecular weight to stain cells and is very suitable for monitoring Mg2+ ions present in the deep tissue. Furthermore, the two-photon probe can be effectively used for the quantitative as well as qualitative detection of intracellular magnesium ions. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular magnesium ions using the two-photon probe.

Abstract: A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20-5 O-fold TPEF enhancement in response to Ca2+, has a dissociation constant (Kd?p) of 0.14±0.02 to 0.25±0.03 ?M, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 ?m in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe.

Abstract: A two-photon probe for real-time monitoring of intracellular free zinc ions is provided. The two-photon probe is represented by Formula 1: wherein R is H or OCH3. The two-photon probe has high selectivity for Zn2+ and enables very effective and long-term monitoring of intracellular free Zn2+ present in the deep tissue. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular free zinc ions using the two-photon probe.

Abstract: Provided are two-photon fluorescent probes for imaging acidic vesicles in live cells and tissue. The probes are represented by The probes can selectively bind to vesicles in cytosol to emit two-photon excited fluorescence with high intensity. Therefore, the use of the probes enables effective imaging of acidic vesicles. Further provided is a method for imaging acidic vesicles in live cells and tissue using the probes.

Abstract: Two-photon dyes for real-time imaging of lipid rafts are provided. The two-photon dyes emit fluorescence with high intensity in the environments of cell membranes and can be used to clearly discriminate the hydrophobic and hydrophilic domains of the cell membranes. Therefore, the use of the two-photon dyes enables satisfactory imaging of actual lipid raft domains. In addition, since the two-photon dyes show no cytotoxicity, they can be suitably used in bioimaging applications, for example, real-time imaging of lipid rafts over a long period of time.