Abstract: A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.

Abstract: The present application relates to methods of producing a protein in fucosydated and afucosylated forms at a predetermined ratio.

Abstract: A coating system and related methods for an airfield surface or a roadway is described. The coating system may include a stable cationic emulsion for application to the airfield surface or the roadway. The stable cationic emulsion may include a) an asphalt blend comprising gilsonite, wherein the gilsonite is modified to possess a positive charge, b) one or more polymers, and c) one or more surfactants not including a cationic surfactant. The coating system may also include a fine aggregate material for application to the stable cationic emulsion applied to the airfield surface or the roadway.

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

Abstract: A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.

Abstract: A coating system and related methods for an airfield surface or a roadway is described. The coating system may include a stable cationic emulsion for application to the airfield surface or the roadway. The stable cationic emulsion may include a) an asphalt blend comprising gilsonite, wherein the gilsonite is modified to possess a positive charge, b) one or more polymers, and c) one or more surfactants not including a cationic surfactant. The coating system may also include a fine aggregate material for application to the stable cationic emulsion applied to the airfield surface or the roadway.

Abstract: The present application relates to methods of producing a protein in fucosylated and afucosylated forms at a predetermined ratio.

Abstract: Wireless pairing is automatically performed based on a purchase of a wireless product. When the wireless product is purchased, a unique product identifier and/or an account number (such as a credit card number) may be used to obtain one or more corresponding wireless network security credentials. Once the wireless network security credentials are identified, the wireless product may be paired to a wireless network based on the wireless network security credentials.

Abstract: Methods and systems for simultaneously forming and filling a container from a preform with fluid, adjusting a fill point of the fluid, and removing air and/or foam from within the container. One of the methods includes injecting fluid from a fill source into the preform through a nozzle passage defined between the nozzle and a seal pin of the forming and filling system to simultaneously form the container from the preform and fill the container. After the container is formed and filled, the seal pin is actuated to close the nozzle passage and prevent fluid and air from exiting the container through the nozzle passage, thereby trapping fluid and air within the container under pressure. Air, fluid, and/or foam is extracted out from within the container through a passageway defined within a stretch rod to adjust a fill point of the container.

Abstract: The present disclosure relates to methods, cells and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interests, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating pyruvate kinase muscle (PKM) expression (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the lactogenic activity of the cell, as well compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using the same.

Abstract: The present disclosure includes proppants and methods of making the proppants. The proppants herein may contain titanium dioxide, silicon dioxide, and/or aluminum dioxide. Also included in the present disclosure are methods of using the proppants to treat a reservoir.

Abstract: A wearable article can include one or more sensors configured to sense one or more of pressure, force, acceleration, and/or tissue activity. The wearable article can also include one or more stimulators configured to generate a magnetic field and positioned to apply the magnetic field to a tissue of a user, i.e., when worn, for treating the tissue after a predetermined pressure, force, acceleration, and/or tissue activity is sensed by the one or more sensors.

Abstract: The present invention provides ethods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

Abstract: Novel tools and techniques might provide for implementing image-based physiological status determination of users, and, in particular embodiments, for implementing physiological status determination of users based on marker-less motion-capture and generating appropriate remediation plans. In various embodiments, one or more cameras may be used to capture views of a user (e.g., an athlete, a person trying to live a healthy life, or the like) as the user is performing one or more set of motions, and the captured images may be overlaid with a skeletal framework that is compared with similar skeletal framework overlaid images for the same one or more sets of motions. The system can automatically determine a physical condition of the user or a probability that the user will suffer a physical condition based at least in part on an analysis of the comparison, which may be difficult or impossible to observe with the naked human eye.

Abstract: Methods are disclosed for determining whether a subject has a synucleinopathy. Methods are also disclosed for detecting misfolded alpha synuclein (?Syn) in a biological sample or fraction thereof. These methods include the use of an ?Syn seeding assay.

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

Abstract: A secure mechanism for adding network devices uses an unsecure guest network and a secure network both coupled to a secure hub. When an unknown device is introduced, it is initially connected to the guest network and can only communicate with the hub and with a wide area network (WAN). The unknown device is prohibited from communicating with the secure network and any device connected to the secure network. The unknown device provides credentials to the hub, which are verified with a secure database, such as a blockchain ledger, that provides manufacturer device information and certification. Upon authentication, the hub permits the identified device to connect to the secure network. The hub may also configure the now identified device for security and operational parameters. The hub may also retrieve network traffic pattern information from the secure database and use such information to monitor normal expected activity from the identified device.

Abstract: An incineration system includes an inlet channel supplying an inlet stream comprising a waste gas containing at least one volatile organic compound, a waste gas sensor measuring at least one property of the waste gas, an oxidizing gas supply controllably providing oxidizing gas to the inlet channel, an incinerator receiving the inlet stream from the inlet channel, an ignitor initiating combustion of the inlet stream in the reaction zone of the incinerator, and a controller receiving data from the waste gas sensor and controlling flow of oxidizing gas from the oxidizing gas supply into the inlet channel. The spiral heat exchanger defines a reaction zone, an incoming path from the inlet channels to the reaction zone, and an outgoing path from the reaction zone to an exhaust channel. The incoming path and the outgoing path extend in alternating concentric spirals with the incoming path being countercurrent to the outgoing path.

Abstract: Device power consumption is reduced by operating using both one-way and two-way communication techniques. In an aspect a device operates in a first mode enabling one-way communication to an other device for a first portion of device operation time. The first mode includes transmitting a first set of messages to the other device while turning off a power supply used by a receiver of the device. The device switches between operating in the first mode and the second mode. The second mode enables two-way communication between the device and the other device for a second portion of device operation time less than the first portion of device operation. The second mode includes transmitting a second message to the other device, and in response to the transmitting the second message, turning on the power supply used by the receiver.

Abstract: The disclosed systems and methods improve on the current landscape surrounding autonomous “programmatic” advertising. The systems and methods incorporate an open source library that advertising publishers can deploy as an auction clearinghouse, setting a universal timeout and comparing all of the responses before sending the winning bids to the publisher's ad server. As an improvement, the present invention further stores the meta data associated with a winning bid on a specialized network for conducting asynchronous communications. This stored or “historical bid data” is then used to improve the speed, efficiency, profitability, and yield of online advertising. In addition, while traditional methodology for pre-bidding only benefits the publishers, the present invention suggests systems and methods wherein users and publishers may participate and be compensated for providing targeted bid data through either FIAT or cryptocurrency.