Abstract: The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.

Abstract: A method for locating an ischemic region in the heart of a subject includes establishing three dimensional coordinates axes with respect to the torso of the subject as a reference; establishing as a reference a multi-dimensional representation of the heart defining at least three dimensional coordinate axes of the heart, the multi-dimensional representation defining at least the base of the heart and a middle section of the heart to thereby prescribe a surface of the heart on the reference multi-dimensional representation of the heart; and orienting the three dimensional coordinate axes of the heart from an initial position offset with respect to the three dimensional coordinates with respect to the torso of the subject to an imaginary position wherein at least one axis of the heart is parallel to or coincident with at least one of the three dimensional coordinate axes with respect to the torso of the subject. Corresponding displays are disclosed also.

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract: Embodiments of the present invention include systems and methods for measuring grain being channeled through a grain harvesting device. The system includes employing a grain test weight measuring apparatus with a weigh bucket for obtaining a test weight measurement of the grain being harvested. The grain test weight measurement apparatus includes a sample cup for weighing grain therein with various sub-assemblies and/or sub-systems to obtain quick and accurate measurements of the grain in-stream on a dynamic platform.

Abstract: A method for locating an ischemic region in the heart of a subject includes establishing three dimensional coordinates axes with respect to the torso of the subject as a reference; establishing as a reference a multi-dimensional representation of the heart defining at least three dimensional coordinate axes of the heart, the multi-dimensional representation defining at least the base of the heart and a middle section of the heart to thereby prescribe a surface of the heart on the reference multi-dimensional representation of the heart; and orienting the three dimensional coordinate axes of the heart from an initial position offset with respect to the three dimensional coordinates with respect to the torso of the subject to an imaginary position wherein at least one axis of the heart is parallel to or coincident with at least one of the three dimensional coordinate axes with respect to the torso of the subject. Corresponding displays are disclosed also.

Abstract: A medical display for analyzing heart signals includes a cardiographic display which displays an electrocardiograph (ECG) heart signal segment of a patient having a magnitude and location in vector format within a single three-dimensional (3D) coordinate system (vectorcardiograph) sampled at incremental time intervals. The display communicates with a central processing unit (CPU) that implements an algorithm to permit a user to selectively and visually display a comparison of the patient ECG with at least one known display in vector format within a single three-dimensional (3D) coordinate system. The display also permits a user to selectively and visually convert and display an ECG heart signal segment into a color-coded projection of a time sequence, A method for analyzing heart signals includes implementing the algorithm to selectively and visually compare the ECG heart signal with at least one known display in vector format selected from the group.

Abstract: A medical display for analyzing heart signals includes a cardiographic display which displays an electrocardiograph (ECG) heart signal segment of a patient having a magnitude and location in vector format within a single three-dimensional (3D) coordinate system (vectorcardiograph) sampled at incremental time intervals. The display communicates with a central processing unit (CPU) that implements an algorithm to permit a user to selectively and visually display a comparison of the patient ECG with at least one known display in vector format within a single three-dimensional (3D) coordinate system. The display also permits a user to selectively and visually convert and display an ECG heart signal segment into a color-coded projection of a time sequence, A method for analyzing heart signals includes implementing the algorithm to selectively and visually compare the ECG heart signal with at least one known display in vector format selected from the group.

Abstract: A process for the purification of viruses from a cell lysate preparation is described, consisting of preferably two successive chromatographic steps; the first a clarification step utilizing size exclusion chromatography, and the second, a virus capture and release step using anion exchange chromatography, which successive chromatographic steps have the advantage of purifying virus, and avoiding chromatography buffer conductivity adjustments.

Abstract: A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.

Abstract: A chemiluminescence-based gel assay may be used as an indicator of fermenter health. The assay is performed using a sample of fermentation medium essentially directly from the fermenter (with no need for complicated purification of the sample before performing the assay). Results from the assay may be correlated with the final product purification yield of a given protein/purification protocol system. This may then be used as a predictor of purification yield before actually performing the purification.

Abstract: A shaving or shearing blade utilized in dressing solder joint chip technologies without the use of heat or a copper block wicking process. In essence, any solder debris resulting from the solder shaving process as implemented by the shaving blade is removed through the intermediary of a vacuum arrangement located as an integral structure in the shaving blade so as to inhibit the potential formation of electrical shorts or causing solder damage in subsequent replacement chip joins or other assembly operations.

Abstract: A shaving or shearing blade utilized in dressing solder joint chip technologies without the use of heat or a copper block wicking process. In essence, any solder debris resulting from the solder shaving process as implemented by the shaving blade is removed through the intermediary of a vacuum arrangement located as an integral structure in the shaving blade so as to inhibit the potential formation of electrical shorts or causing solder damage in subsequent replacement chip joins or other assembly operations.

Abstract: Apparatus and method is provided for separating electrical connections, such as, solder connections, between two surfaces in which electrical connections are relatively weak and thus can be readily separated. The preferred application of the disclosed process and apparatus is its use in separating semiconductor devices from its device carrier after the devices have been through burn-in tests. The unique process and apparatus allows multiple uses of device carrier for testing and/or burning-in devices.

Abstract: A cardiographic display for displaying an electrocardiograph heart signal in vector format within a single three-dimensional coordinate system sampled at incremental time intervals and combining the above vector display on the same screen with other displays, e.g., a 12-Lead display. Other embodiments of the present invention comprise projecting the results of the heart vectors onto three planes of the coordinate system which represent the frontal, transverse and sagittal planes while simultaneously displaying the three-dimensional vector display. Still other embodiments comprise combining the three-dimensional with various graphs which show the various changes in magnitude and angle between the heart vectors.

Abstract: Apparatus and method is provided for separating electrical connections, such as, solder connections, between two surfaces in which electrical connections are relatively weak and thus can be readily separated. The preferred application of the disclosed process and apparatus is its use in separating semiconductor devices from its device carrier after the devices have been through burn-in tests. The unique process and apparatus allows multiple uses of device carrier for testing and/or burning-in devices.

Abstract: Apparatus and method is provided for separating electrical connections, such as, solder connections, between two surfaces in which electrical connections are relatively weak and thus can be readily separated. The preferred application of the disclosed process and apparatus is its use in separating semiconductor devices from its device carrier after the devices have been through burn-in tests. The unique process and apparatus allows multiple uses of device carrier for testing and/or burning-in devices.

Abstract: A signal of bandwidth F.sub.max is unambiguously transmitted over a datalink having a bandwidth less than F.sub.max by subdividing the signal into a plurality of subspectrums and bandshifting the subspectrums greater than the datalink bandwidth to baseband. Each bandshifted subspectrum is uniquely encoded using a different pilot tone which can be detected by the receiver to decode the receive signal and unambiguously recover the narrowband spectrum components.