Abstract: The subject disclosure presents systems and computer-implemented methods for determining an acoustic time-of-flight (TOF) of sound waves through a sample material with greater accuracy and in a more repeatable fashion, by invoking one or more of an envelope generation for an error function, fitting a non-linear curve to an ultrasound frequency sweep, or performing a clustered piece-wise linear regression on individual linear parts of the ultrasonic frequency sweep. The systems and methods are useful for, among other things, monitoring diffusion of fluids through porous materials, such as tissue samples.

Abstract: The subject disclosure presents systems and computer-implemented methods for determining an acoustic time-of-flight (TOF) of sound waves through a sample material with greater accuracy and in a more repeatable fashion, by invoking one or more of an envelope generation for an error function, fitting a non-linear curve to an ultrasound frequency sweep, or performing a clustered piece-wise linear regression on individual linear parts of the ultrasonic frequency sweep. The systems and methods are useful for, among other things, monitoring diffusion of fluids through porous materials, such as tissue samples.

Abstract: The subject disclosure presents systems and computer-implemented methods for calculating the diffusivity constant of a sample using acoustic time-of-flight (TOF) based information correlated with a diffusion model to reconstruct a sample's diffusivity coefficient. Operations disclosed herein such as acoustically determining the phase differential accumulated through passive fluid exchange (i.e. diffusion) based on the geometry of the tissue sample, modeling the impact of the diffusion on the TOF, and using a post-processing algorithm to correlate the results to determine the diffusivity constant, are enabled by monitoring the changes in the speed of sound caused by penetration of fixative such as formalin into several tissue samples. A tissue preparation system may be adapted to monitor said diffusion of a tissue sample and determine an optimal processing workflow.

Abstract: A method can be utilized to operate an elevator installation comprising at least two elevator shafts, an elevator cabin, a control system, and a shaft changeover unit. With regard to the elevator cabin, the shaft changeover unit can adopt an enabled state in which entry into the shaft changeover unit is permitted and a disabled state in which entry into the shaft changeover unit is blocked. A travel route from a start position in the shaft system to a destination position in the shaft system via the shaft changeover unit is determined for the elevator cabin, whereby the elevator cabin is moved proceeding from the start position, and the control system controls a travel parameter of the elevator cabin such that the elevator cabin, proceeding from the start position, reaches the shaft changeover unit when the shaft changeover unit is in the enabled state for the elevator cabin.

Abstract: The subject disclosure presents systems and computer-implemented methods for evaluating a tissue sample that has been removed from a subject. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The rate of change in the speed of the energy is correlated with the extent of diffusion. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A time-of-flight of acoustic waves and rate of change thereof is monitored to determine an optimal time for soaking the tissue sample in a fixative.

Abstract: An elevator system includes at least one guide rail and at least one elevator cabin movable in a direction of travel along the guide rail. A cabin control unit is installed on the elevator cabin and a central control unit is connected to the cabin control unit by a wireless communications system. The wireless communications system includes a slotted waveguide conductor arrangement installed in the elevator shaft.

Abstract: The subject disclosure presents systems and computer-implemented methods for evaluating a tissue sample that has been removed from a subject. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The rate of change in the speed of the energy is correlated with the extent of diffusion. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A time-of-flight of acoustic waves and rate of change thereof is monitored to determine an optimal time for soaking the tissue sample in a fixative.

Abstract: The subject disclosure presents systems and computer-implemented methods for calculating the diffusivity constant of a sample using acoustic time-of-flight (TOF) based information correlated with a diffusion model to reconstruct a sample's diffusivity coefficient. Operations disclosed herein such as acoustically determining the phase differential accumulated through passive fluid exchange (i.e. diffusion) based on the geometry of the tissue sample, modeling the impact of the diffusion on the TOF, and using a post-processing algorithm to correlate the results to determine the diffusivity constant, are enabled by monitoring the changes in the speed of sound caused by penetration of fixative such as formalin into several tissue samples. A tissue preparation system may be adapted to monitor said diffusion of a tissue sample and determine an optimal processing workflow.

Abstract: A drive arrangement that includes a movable rail segment of a lift system, an electric motor configured to rotate the rail segment through a prescribed maximum movement path, and inverter units for making available electrical power to the electric motor and configured to receive a control command relating to the position of the movable rail segment and to make available electrical drive power based thereon. The drive arrangement may form at least two drive segments each of which includes one of the inverter units and a coil arrangement that is supplied with electrical power by the assigned inverter unit. Each of the drive segments may be configured such that the inverter units operate with equal priority.

Abstract: The subject disclosure presents systems and computer-implemented methods for calculating the diffusivity constant of a sample using acoustic time-of-flight (TOF) based information correlated with a diffusion model to reconstruct a sample's diffusivity coefficient. Operations disclosed herein such as acoustically determining the phase differential accumulated through passive fluid exchange (i.e. diffusion) based on the geometry of the tissue sample, modeling the impact of the diffusion on the TOF, and using a post-processing algorithm to correlate the results to determine the diffusivity constant, are enabled by monitoring the changes in the speed of sound caused by penetration of fixative such as formalin into several tissue samples. A tissue preparation system may be adapted to monitor said diffusion of a tissue sample and determine an optimal processing workflow.

Abstract: Provided herein are 2?-O-methyl 3?phosphorothioate (MS)-modified synthetic nucleic acid molecules (single guide RNAs (sgRNAs)) for the use with a Cas9 nuclease in combination as a ribonucleoprotein (RNP) complex for an electroporation-based ex vivo targeted gene disruption of the BCL11A erythroid enhancer's +55, +58, or +62, functional regions. Additionally, provided herein are said RNP complexes (Cas9:MS-sgRNA), and compositions comprising the modified synthetic nucleic acid molecules or said RNP complexes, and methods for increasing fetal hemoglobin levels in a cell by disrupting BCL11A expression at the genomic level. Also provided herein are methods and compositions relating to the treatment of hemoglobinopathies by reinduction of fetal hemoglobin levels.

Abstract: A mechanism for declarative service domain federation uses a declarative approach to both the visibility aspect and the management aspect of service domain federation. Instead of manually exporting services, that is, selecting individual services from source domains to be visible in the target domain, using the present system, a federation architect uses a federation console to specify federation intent. The federation intent may include federation rule, query-based service group, and quality-of-service (QoS) policy. Based on the declared federation intent, a planning engine resolves the individual services based on the group query, generates the service visibility configuration, and creates the necessary service proxies and/or mediation objects to satisfy the goal. At runtime, a service monitor collects the performance metrics of federated services and dynamically adjusts the mediation/proxy configurations to maintain the QoS objectives specified by the federation architect.

Abstract: A drive arrangement includes a movable, rotatable rail segment of an elevator system. An electric motor moves the movable rail segment. The drive arrangement is configured to rotate the rail segment about an angle of rotation of less than 360°. An inverter unit provides electrical power to the electric motor and is configured to receive a control command relating to the position of the movable rail segment and provide the electrical drive power based on the control command. The drive arrangement forms two or exactly three drive segments. Each drive segment includes an inverter unit and a coil arrangement, which is supplied with electrical power by the assigned inverter unit. Each inverter unit includes a communication unit, which receives the control command. The communication units are configured to specify amongst themselves a master communication unit and to specify the remaining communication unit as slave communication units.

Abstract: A method of evaluating the quality state (such as a fixation status) of a cellular sample is provided. A MIR spectrum (220) of the sample is obtained, and a classification (211) or quantification (231) algorithm is applied to the MIR spectrum to identify features (221) indicative of the quality state and/or to classify the sample. The quality state may then be used to determine whether the sample is appropriate for an analytical method and/or whether remedial processing (such as further fixation) is appropriate.

Abstract: A method and system are described for processing tissues according to particular processing protocols that are established based on time-of-flight measurements as a processing fluid is diffused into a tissue sample. In one embodiment, measurement of the time it takes about 70% ethanol to diffuse into a tissue sample is used to predict the time it will take to diffuse other processing fluids into the same or similar tissue samples. Advantageously, the disclosed method and system can reduce overall processing times and help ensure that only samples that require similar processing conditions are batched together.

Abstract: An elevator system having a car which can be moved along an elevator shaft in a direction of travel. The elevator shaft includes a busbar that extends in the direction of the direction of travel. The car comprises a current collector that can be moved between a first position and a second position along a compression direction. In the first position, the current collector is in engagement with the busbar such that there is an electrical connection between the busbar and the current collector. In the second position, the current collector is disengaged from the busbar, such that the electrical connection between the busbar and the current collector is separated.

Abstract: A method is disclosed that permits calculation of reagent concentrations (in SI units) over time and space within a tissue sample as the sample is immersed in the reagent and the reagent diffuses into the tissue sample. The disclosed method has yielded the surprising result that once a formaldehyde concentration at all points within a tissue sample exceeds about 90 mM during a cold step of a cold+hot fixation protocol, the hot step of the fixation protocol can be commenced to provide reliable detection of molecular targets and preservation of tissue morphology in downstream analyses.

Abstract: A testing arrangement serves for controlling the manufacturing of a fiber reinforced component. The testing arrangement includes an inspection device with a sensor system, with the inspection device being configured to move on and/or above one or more material layer(s) and/or a surface of the component and to examine the material layer(s) or the surface with the sensor system. A device serves for automated manufacturing of a fiber reinforced plastic laminate. The device includes a forming tool, a laying head for automated laying of material layers in the forming tool as well as a testing arrangement.

Abstract: In order to provide a simplified inspection of an aircraft for the pilot, an aircraft inspection system is provided which includes at least one movable inspection unit, a position detection arrangement, and at least one data transfer interface. The at least one moveable inspection unit is moveable relative to an aircraft to be inspected. The at least one movable inspection unit includes at least one sensor for detecting a characteristic value, for verifying a characteristic and/or for determining a defect of an aircraft. The movable inspection unit is configured to generate monitoring data. When a defect or a characteristic value is detected, the position detection arrangement detects position data of the movable inspection unit in relation to the aircraft to be inspected, and assigns the position data to the monitoring data. The data transfer interface provides the position data with the assigned monitoring data as inspection data.

Abstract: Methods of treating an individual who has been identified as having glycolysis dependent cancer are disclosed. The methods comprise the step of: administering to suc an individual a combination of an anti-cancer composition that renders the cancer incapable of glycolysis and an autophagy inhibitor. Pharmaceutical compositions and kits comprising that renders the cancer incapable of glycolysis and an autophagy inhibit are also disclosed. Methods of treating an individual who has a disease characterized b cell degeneration and cell death due to autophagy are disclosed. The methods comprise administering to the individual a permeable form of a metabolic substrate that can be oxidized in the tricarboxylic acid cycle to produce NADH. Methods for identifying an autophagy inhibitor comprising performing a test assay using an apopto sis-resistant cell are disclosed.