Abstract: Methods and systems for designing proteins are disclosed, as well as proteins and protein assemblies designed. A computing device can determine a protein repeating unit that includes one or more protein helices and one or more protein loops. The computing device can generate a protein backbone structure with a copy of the protein repeating unit. The computing device can determine whether a distance between a pair of helices of the protein backbone structure is between lower and upper distance thresholds. After determining that the distance between the pair of helices is between the lower and upper distance thresholds, the computing device can: generate a plurality of protein sequences based on the protein backbone structure, select a particular protein sequence of the plurality of protein sequences based on an energy landscape that has information about energy and distance from a target fold of the particular protein sequence, and generate an output based on the particular protein sequence.

Abstract: The present disclosure provides transferring receptor binding polypeptides of the general formula H1-H2-E1-H3-E2-E3-H-4, herein H1, H2, H3, and H4 each independently comprise an alpha, helical domain of between 11-20 amino acids in length; E1, E2, and E3 each independently comprise a beta sheet of 5 amino acids in length; and optional amino acid linkers between domains.

Abstract: Disclosed herein are nanostructures and their use, where the nanostructures include (a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides; (b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptide differs from the first polypeptide; wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructures; and wherein the nanostructure displays multiple copies of one or more paramyxovirus and/or pneumovirus F proteins or antigenic fragments thereof, on an exterior of the nanostructure.

Abstract: Polypeptide inhibitors of SARS-COV-2 are disclosed comprising an amino acid sequence at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to the amino acid sequence selected from the group consisting of SEQ ID NOS: 1-17, 19-21, 23-34 and 100-101, and their use for treating and limiting development of SARS-COV-2 infection.

Abstract: Antibody particles are disclosed comprising polypeptides comprising an (Fc) binding domain, a helical polypeptide monomer, and an oligomer domain, and either Tie2 antibodies or dimers, or tumor necrosis factor receptor superfamily antibodies, and uses thereof.

Abstract: Disclosed herein are polypeptide comprising an amino acid sequence at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to the amino acid sequence of any one of SEQ ID NOS: 1-4, oligomers thereof, and uses thereof.

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.

Abstract: De novo designed polypeptides that bind to IL-2 receptor ?c heterodimer (IL-2R?c), IL-4 receptor ?c heterodimer (IL-4R?c), or IL-13 receptor ? subunit (IL-13R?) are disclosed, as are methods for using and designing the polypeptides.

Abstract: Disclosed herein are junction polypeptides that can be used, for example, to join together protein building blocks via a rigid fusion to generate a wide range of protein shapes; fusion proteins comprising such junction polypeptides, polymers thereof, and methods for designing such junction polypeptides.

Abstract: Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:1, are provided, wherein (a) the polypeptide degrades a PFQPQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4; (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp; and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T, 368F/Q, 399, 402, 406, 424, 449, 461, 463, 105, 171, 172, 173, 174, and 456.

Abstract: Polypeptides including an amino acid sequence at least 50% identical to the amino acid sequence selected from the group consisting of SEQ ID NOS: 1-3297 are provided, which are capable of forming protein homodimers containing tunable symmetric pockets.

Abstract: Disclosed herein are ?-barrel polypeptides including self-complementing multipartite ?-barrel polypeptides and circularly permuted ?-barrel polypeptides and methods for their design and use in mediating real-time monitoring of polypeptide-polypeptide association and dissociation events.

Abstract: An aircraft includes a fuselage, a wing connected to and extending outward from the fuselage, and an engine mounted to the wing. The engine includes turbomachine defining a centerline axis, a fan, and an exhaust section with an outlet nozzle. The turbomachine defines a centerline axis. The fan is connected to and is disposed upstream from the turbomachine. The fan is disposed to rotate about the centerline axis. During operation of the engine, an exhaust stream is expelled from the outlet nozzle of the exhaust section. The exhaust stream defines a mean direction of flow in the downstream direction from the exhaust section. The mean direction of flow defines a first angle with the centerline axis of the turbomachine that is greater than zero such that the centerline axis is oriented downwardly along the vertical direction relative to the mean direction of flow of the exhaust stream.

Abstract: A method of conveying a production fluid from an offshore subsea well to an offshore vessel includes deploying an inflatable bladder from the offshore vessel, the inflatable bladder including a bladder valve, and fluidly connecting the inflatable bladder to an offloading port positioned at a seafloor, wherein the offloading port includes a port valve and is in fluid communication with one or more subterranean hydrocarbon-bearing formations. The method further includes opening the bladder and port valves to discharge the production fluid from the offloading port into the inflatable bladder, and thereby resulting in a substantially filled bladder, closing the bladder and port valves, and fluidly disconnecting the substantially filled bladder from the offloading port.

Abstract: A lawn mower may include a blade housing configured to house at least a first blade and a second blade, a shaft disposed to rotate within the blade housing, a powerhead configured to selectively turn the shaft responsive to application of power from a power source, and a blade coupler configured to selectively couple the first blade and/or the second blade to the shaft to produce different cutting performance conditions based on which of the first blade and/or the second blade is operably coupled to the shaft.

Abstract: Techniques are described in which sensor data is used to determine one or more of background noise or occupancy associated with a passenger cabin of a vehicle. The sensor data, in turn, is used to determine an operating state for one or more components of the vehicle (e.g., pumps, compressors, fans, blowers, etc.) such that an amount of background noise within the passenger cabin is reduced (e.g., when a passenger/occupant is present). In various examples, the operating state of the component may operate in a different, though louder, state (e.g., higher efficiency, greater power, etc.) when an occupant is not present or proximate the component.

Abstract: A system for deploying an implant cut from a biological tissue into an eye of a patient including a delivery device and a nose cone assembly, a tubular shaft projecting from the distal end region of the nose cone and comprising a lumen. Related devices, systems, and methods are provided.

Abstract: Disclosed herein are macrocyclic polypeptides having no more than 3 ammo acid substitutions compared to the amino acid sequence of any one of SEQ ID NO: 1-2.37 or a mirror image thereof, wherein the polypeptide includes both L and D amino acids, libraries of such polypeptides, and uses thereof.