Abstract: Embodiments disclosed herein provide methods for identifying new CRISPR loci and effectors, as well as different CRISPR loci combinations found in various organisms. Class-II CRISPR systems contain single-gene effectors that have been engineered for transformative biological discovery and biomedical applications. Discovery of additional single-gene or multi-component CRISPR effectors may enhance existing CRISPR applications, such as precision genome engineering. Comprehensive characterization of CRISPR-loci may identify novel functional roles of CRISPR loci enabling new tools for biomedicine and biological discovery. CRISPR loci have enormous feature complexity, but classification of CRISPR loci has been focused on a small fraction of highly abundant features. Increased genome sequencing has enhanced the sampling of this feature complexity.

Abstract: In one embodiment, a method includes receiving a traffic flow including a plurality of packets encrypted using a cryptographic protocol, determining cryptographic protocol data of the traffic flow, and transmitting telemetry data of the traffic flow including the cryptographic protocol data. In another embodiment, a method includes receiving telemetry data of a traffic flow including a plurality of packets encrypted using a cryptographic protocol, the telemetry data including cryptographic protocol data of the traffic flow, classifying the traffic flow based on the cryptographic protocol data using a machine learning classifier; and taking a remedial action with respect to the traffic flow based on the classification of the traffic flow.

Abstract: Systems and methods for pacing cardiac conductive tissue are described. In an embodiment, a medical system includes an electrostimulation circuit to generate pacing pulses to stimulate a His bundle or a bunch branch. A sensing circuit senses a far-field ventricular activation, determines a cardiac synchrony indicator using the far-field ventricular activation in response to His bundle or bundle branch pacing, and verifies His-bundle capture status using the determined cardiac synchrony indicator. The system can determine a pacing threshold using the capture status under different stimulation strength values. The electrostimulation circuit can deliver stimulation pulses in accordance with the determined pacing threshold.

Abstract: Multibiotic agents are disclosed. The multibiotic agents may contain two or more moieties linked through bonds cleavable in vivo. The bonds cleavable in vivo can be ester bonds, amide bonds, azo bonds, glycosidic bonds, carbonate linkers, or carbamate linkers. The moieties can be alcohol cores, amine cores, and/or acyls. Also disclosed are compositions containing multibiotic agents and methods of using the multibiotic agents.

Abstract: Disclosed herein are therapeutic compositions containing non-pathogenic, germination-competent bacterial spores, for the prevention, control, and treatment of gastrointestinal diseases, disorders and conditions and for general nutritional health.

Abstract: Systems and methods for pacing cardiac conductive tissue are described. In an embodiment, a medical system includes an electrostimulation circuit to generate pacing pulses to stimulate a His bundle or a bunch branch. A sensing circuit senses a far-field ventricular activation, determines a cardiac synchrony indicator using the far-field ventricular activation in response to His bundle or bundle branch pacing, and verifies His-bundle capture status using the determined cardiac synchrony indicator. The system can determine a pacing threshold using the capture status under different stimulation strength values. The electrostimulation circuit can deliver stimulation pulses in accordance with the determined pacing threshold.

Abstract: Provided are therapeutic compositions containing Ecobiotic™ populations for prevention, treatment and reduction of symptoms associated with a dysbiosis of a mammalian subject such as a human.

Abstract: Vision systems for autonomous machines and methods of using same during machine localization are provided. Exemplary systems and methods may reduce computing resources needed to perform vision-based localization by selecting the most appropriate camera from two or more cameras, and optionally selecting only a portion of the selected camera's field of view, from which to perform vision-based location correction. Other embodiments may provide camera lens coverings that maintain optical clarity while operating within debris-filled environments.

Abstract: The present disclosure relates to apparatus and methods for performing maskless lithography processes. A substrate rocessing apparatus includes a slab with a plurality of guiderails coupled to and extending along the slab. A first shuttle is disposed on the plurality of guiderails, a second shuttle is disposed on the first shuttle, and a metrology bar is coupled to the second shuttle. The etrology bar includes a first plurality of sensors coupled to the metrology bar. A second plurality of sensors coupled to the metrology bar are disposed laterally inward of the first plurality of sensors.

Abstract: A computer system provides at least one workspace as a user interface corresponding to a user-generated topic. A home topic is provided to the user as an initially selected topic. The home topic provides a workspace to the user as a user interface. User selection of a first topic within the home topic causes designation of the first topic as the current topic. A first workspace is associated with the first topic, and a first topic data structure associated with the first topic is generated and the first topic data structure is associated only with application windows, files and application related data used by the user while the first topic is designated as the current topic. The user is provided, within the first workspace, only application windows, files and application related data used by the user while the first topic is designated as the current topic.

Abstract: The disclosure relates generally to uses of tRNA-based effector molecules having a non-naturally occurring modification.

Abstract: The present disclosure provides compositions with a modulating gene expression and methods for modulating transcription.

Abstract: Provided are therapeutic compositions containing combinations of bacteria, for the maintenance or restoration of a healthy microbiota in the gastrointestinal tract of a mammalian subject, and methods for use thereof.

Abstract: A novel fuse assembly design utilizes a fuse body, a single-piece terminal assembly and a two-piece endbell. The terminal assembly is disposed within the fuse body and includes first and second opposing surfaces with a fuse element extending between a first terminal and a second terminal. The endbell, to be connected to the fuse body, includes first and second endbell portions. Formed within the first endbell portion is a first receptacle and extending from the first endbell portion is a first protrusion. Formed within the second endbell portion is a second receptacle and extending from the second endbell portion is a second protrusion. When the two endbell portions are fastened to one another with the terminal assembly sandwiched between them, the first protrusion engages the second receptacle and the second protrusion engages the first receptacle.

Abstract: Steering is used to augment the CMG-based balance control of a two-wheeled vehicle, e.g., a bicycle, electric bicycle (“ebike”), scooter, electric scooter, moped, or motorcycle. A control architecture enables a two wheeled vehicle with simultaneously or alternating mechatronic attitude control systems to balance autonomously at rest or while dynamically driven with mechatronic command.

Abstract: A system for compensating for phase noise, with particular application in lidar, includes a compensation interferometer that receives a signal from a source, and splits it into a first and second path, with a path length difference ?? between them. Typically the path length is significantly less than that of the return distance to a target. The output of the compensation interferometer, which consists of phase noise generated in time ?? is vectorially summed during a time similar to a signal flight time to a target, and the result used to reduce phase noise present on measurements of a target. It further includes means for selecting ?? such that competing noise elements are reduced or optimised.

Abstract: The present disclosure provides compositions with a modulating gene expression and methods for modulating transcription.

Abstract: A low pressure gear pump and wear plate is disclosed. The wear plate may comprise a sidewall, a drive portion, a driven portion, and a transition portion. The sidewall is free of a sealing member or a recess configured to receive the sealing member. The drive portion includes a drive inlet lip, a drive outlet lip, a drive recessed trough and a drive bore. The drive bore is configured to receive the drive shaft of the gear pump. The driven portion may that include a driven inlet lip, a driven outlet lip, a driven recessed trough, and a driven bore. The driven bore is configured to receive the driven shaft of the gear pump. The transition portion may include a transition aperture configured to receive a first fastener configured to mount the wear plate to the gear housing. The wear plate is made of bronze, aluminum or non-magnetic material.

Abstract: Embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type V effector protein. In certain other example embodiments, the Type V effector protein is Cpf1. Embodiments disclosed herein are directed to viral vectors for delivery of CRISPR-Cas effector proteins, including Cpf1. In certain example embodiments, the vectors are designed so as to allow packaging of the CRISPR-Cas effector protein within a single vector. There is also an increased interest in the design of compact promoters for packing and thus expressing larger transgenes for targeted delivery and tissue-specificity.