Patents by Inventor David C. Duffy
David C. Duffy has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11977087Abstract: Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like.Type: GrantFiled: August 6, 2021Date of Patent: May 7, 2024Assignee: Quanterix CorporationInventors: David Fournier, Todd Campbell, Cheuk W. Kan, John Lawson, Andrew Rivnak, Michael Kagan, David C. Duffy
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Publication number: 20230213510Abstract: Described herein are methods for improving the accuracy of capture object-based assays. In some embodiments, a measure of the number or a measure of the concentration of an analyte molecule or particle in a fluid sample is determined using the capture object-based assay.Type: ApplicationFiled: December 8, 2022Publication date: July 6, 2023Applicant: Quanterix CorporationInventors: David C. Duffy, David M. Rissin, Matthew Fishburn, Andrew Rivnak, Purvish Patel
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Publication number: 20230109130Abstract: Methods and systems for capture object-based assays, including for determining a measure of the concentration of an analyte molecule or particle in a fluid sample, are described. The methods and systems may relate to high sensitivity detection of analytes, sometimes using assay conditions and sample handling that result in the capture and detection of a high percentage of the analyte molecules or particles in a fluid sample using relatively few capture objects. Apparatuses and methods for immobilizing capture objects with respect to assay sites, in some instances with unexpectedly high efficiencies are also described. Some such apparatuses involve the use of force fields and fluid meniscus forces, alone or in combination, to facilitate or improve capture object immobilization.Type: ApplicationFiled: October 13, 2022Publication date: April 6, 2023Applicant: Quanterix CorporationInventors: David C. Duffy, Nicholas Collier, Cheuk W. Kan, Carmen I. Tobos, David M. Rissin, Alexander D. Wiener, Ray Meyer, Christopher Warwick, Roger Millington, Danielle M. Svancara
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Patent number: 11619631Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.Type: GrantFiled: April 11, 2019Date of Patent: April 4, 2023Assignee: Quanterix CorporationInventors: David C. Duffy, David M. Rissin, David R. Walt, David Fournier, Cheuk Kan
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Patent number: 11434264Abstract: Ultrasensitive and quantitative assays for detecting toxins of Clostridium difficile, which may involve digital ELISA, are provided. Also provided herein are differential detection assays that allow for distinguishing toxin B of highly virulent Clostridium difficile strains from toxin B of less virulent strains.Type: GrantFiled: February 12, 2016Date of Patent: September 6, 2022Assignees: Quanterix Corporation, Beth Israel Deaconess Medical Center, Inc.Inventors: Nira Pollock, Ciaran Kelly, David C. Duffy, Linan Song, Mingwei Zhao
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Publication number: 20220205992Abstract: Described herein are materials and kits for associating species with a surface of an object. In some embodiments, the object comprises a plurality of capture objects (e.g., beads).Type: ApplicationFiled: December 28, 2020Publication date: June 30, 2022Applicant: Quanterix CorporationInventors: David M. Rissin, David C. Duffy
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Publication number: 20220034917Abstract: Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like.Type: ApplicationFiled: August 6, 2021Publication date: February 3, 2022Applicant: Quanterix CorporationInventors: David Fournier, Todd Campbell, Cheuk W. Kan, John Lawson, Andrew Rivnak, Michael Kagan, David C. Duffy
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Publication number: 20210311058Abstract: Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.Type: ApplicationFiled: March 23, 2021Publication date: October 7, 2021Applicant: Quanterix CorporationInventors: David M. Rissin, David Fournier, David C. Duffy
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Patent number: 11112415Abstract: Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like.Type: GrantFiled: March 14, 2018Date of Patent: September 7, 2021Assignee: Quanterix CorporationInventors: David Fournier, Todd Campbell, Cheuk Kan, John Lawson, Andrew Rivnak, Michael Kagan, David C. Duffy
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Patent number: 10989713Abstract: Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.Type: GrantFiled: November 10, 2017Date of Patent: April 27, 2021Assignee: Quanterix CorporationInventors: David M. Rissin, David Fournier, David C. Duffy
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Publication number: 20200393457Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.Type: ApplicationFiled: June 19, 2020Publication date: December 17, 2020Applicant: Quanterix CorporationInventors: David C. Duffy, David M. Rissin, David R. Walt, David Fournier, Cheuk W. Kan
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Publication number: 20200271643Abstract: Methods and systems for the detection of analyte molecules are generally described. In some embodiments, a method may comprise using a stabilizing agent prior to, during, and/or after one or more steps of a detection assay. The stabilizing agent may serve to maintain and/or increase the detectable signal indicative of the analyte during one or more assay steps and/or until the signal is measured. In such cases, the stabilizing agent may reduce and/or prevent one or more phenomena associated with signal decay, such as, e.g., dissociation between, aggregation of, and/or denaturation of analyte molecules and/or detection molecules. In some embodiments, the stabilizing agent can be used to improve the sensitivity of new and existing assays with little or no adverse effect on specificity. The methods and systems, described herein, may be used for a plethora of applications, including the detection or quantification of low levels of analyte molecules.Type: ApplicationFiled: September 20, 2018Publication date: August 27, 2020Applicant: Quanterix CorporationInventors: David Wilson, David M. Rissin, Lei Chang, Andrew Rivnak, David C. Duffy
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Patent number: 10725032Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.Type: GrantFiled: April 14, 2017Date of Patent: July 28, 2020Assignee: Quanterix CorporationInventors: David C. Duffy, David M. Rissin, David R. Walt, David Fournier, Cheuk Kan
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Patent number: 10640814Abstract: Described herein are methods and systems for detecting DNA or RNA using single molecules array or other techniques. DNA or RNA from the sample may be fragmented and exposed to a first type of binding ligand and a second type of binding ligand that comprise nucleic acid sequences complimentary at least a portion of a sequence contained in the target DNA or RNA. At least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand and second type of binding ligand comprises nucleic acid sequences complimentary to a different portions of a sequence contained in the DNA or RNA. A portion of the sample exposed to the binding ligands is analyzed to determine the number of fragmented DNA or RNA sequences.Type: GrantFiled: March 30, 2018Date of Patent: May 5, 2020Assignee: Quanterix CorporationInventors: David C. Duffy, Linan Song, Dandan Shan, Mingwei Zhao
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Publication number: 20200123592Abstract: Methods for detecting nucleic acids, particularly small nucleic acids such as microRNAs, involving the use of a peptide nucleic acid (PNA) probe that lacks a base and a labelled modified base corresponding to the omitted base in the PNA probe. A complex containing the target nucleic acid, the PNA probe, and the modified base can be determined using a single molecule array assay.Type: ApplicationFiled: May 29, 2018Publication date: April 23, 2020Applicants: Quanterix Corporation, DestiNA Genomics Ltd.Inventors: Juan J. Díaz-Mochón, David C. Duffy, Salvatore Pernagallo, Hugh llyine, David M. Rissin, Barbara López-Longarela
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Publication number: 20190302109Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.Type: ApplicationFiled: April 11, 2019Publication date: October 3, 2019Applicant: Quanterix CorporationInventors: David C. Duffy, David M. Rissin, David R. Walt, David Fournier, Cheuk Kan
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Publication number: 20180363038Abstract: Described herein are methods and systems for detecting DNA or RNA using single molecules array or other techniques. DNA or RNA from the sample may be fragmented and exposed to a first type of binding ligand and a second type of binding ligand that comprise nucleic acid sequences complimentary at least a portion of a sequence contained in the target DNA or RNA. At least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand and second type of binding ligand comprises nucleic acid sequences complimentary to a different portions of a sequence contained in the DNA or RNA. A portion of the sample exposed to the binding ligands is analyzed to determine the number of fragmented DNA or RNA sequences.Type: ApplicationFiled: March 30, 2018Publication date: December 20, 2018Applicant: Quanterix CorporationInventors: David C. Duffy, Linan Song, Dandan Shan, Mingwei Zhao
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Publication number: 20180306830Abstract: Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like.Type: ApplicationFiled: March 14, 2018Publication date: October 25, 2018Applicant: Quanterix CorporationInventors: David Fournier, Todd Campbell, Cheuk Kan, John Lawson, Andrew Rivnak, Michael Kagan, David C. Duffy
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Publication number: 20180224451Abstract: Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.Type: ApplicationFiled: November 10, 2017Publication date: August 9, 2018Applicant: Quanterix CorporationInventors: David M. Rissin, David Fournier, David C. Duffy
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Patent number: 9952237Abstract: Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like.Type: GrantFiled: February 25, 2011Date of Patent: April 24, 2018Assignee: Quanterix CorporationInventors: David Fournier, Todd Campbell, Cheuk Kan, John Lawson, Andrew Rivnak, Michael Kagan, David C. Duffy