Abstract: The subject matter disclosed herein is generally directed to methods and systems for screening phenotypes associated with genetic elements and identifying genetic elements at the single-cell level using optical barcodes. A major advantage offered by this approach is the ability to screen for any cellular phenotype that can be identified by high-resolution microscopy—including live-cell phenotypes, protein localization, or highly multiplexed expression profile and mRNA localization in conjunction with a large array of genetic elements applied as a pool in a single test volume.

Abstract: A ring for a fighting sport has a circular fighting area defined by a plurality of circular fence rings that are held up by posts. Shock absorbers connect the fence rings to the posts. Position markers are provided to indicate the starting positions of the participating fighters at certain points during the bout.

Abstract: The invention provides for use of CRISPR-Cas systems to sort barcoded cells or molecules. Cells or nucleic acid molecules may be sorted from a heterogenous population by targeting a barcode of interest specific for a cell or cell progeny or nucleic acid molecule of interest.

Abstract: Embodiments disclosed herein are directed to a new genetic perturbation and screening method that combines advantages of pooled perturbation with imaging assays for complex phenotypes. Specifically, the method may be used to screen pooled genomic perturbations to identify phenotypes and to identify perturbed genes at the single-cell level using optical barcodes. A major advantage offered by this approach is the ability to screen for any cellular phenotype that can be identified by high-resolution microscopy—including live-cell phenotypes, protein localization, or highly multiplexed expression profile and mRNA localization by RNA-FISH—in conjunction with a large array of genetic perturbations applied as a pool in a single test volume.

Abstract: Embodiments disclosed herein are directed to a new genetic perturbation and screening method that combines advantages of pooled perturbation with imaging assays for complex phenotypes. Specifically, the method may be used to screen pooled genomic perturbations to identify phenotypes and to identify perturbed genes at the single-cell level using optical barcodes. A major advantage offered by this approach is the ability to screen for any cellular phenotype that can be identified by high-resolution microscopy—including live-cell phenotypes, protein localization, or highly multiplexed expression profile and mRNA localization by RNA-FISH—in conjunction with a large array of genetic perturbations applied as a pool in a single test volume.

Abstract: The subject matter disclosed herein is generally directed to methods and compositions for tagging cells of interest, tracking evolution of the tagged cells, and recovering the original tagged cells for further study. Specifically, cells are tagged with a DNA construct encoding a barcode sequence comprising a guide sequence. Barcoded cells can then be recovered using a reporter construct having CRISPR target sequences specific for the cell having a barcode of interest.

Abstract: A ring for a fighting sport has a circular fighting area defined by a plurality of circular fence rings that are held up by posts. Shock absorbers connect the fence rings to the posts. Position markers are provided to indicate the starting positions of the participating fighters at certain points during the bout.

Abstract: Embodiments disclosed herein provide reagents and methods for high-throughput screening of nucleic acid sequence variations in nucleic acid containing specimens. Nucleic acid specimens to be screened are loaded into separate discrete volumes. Optically encoded particles are used to deliver primers to amplify one or more sequences comprising the nucleic acid sequence variation. The optically encoded particles may be delivered to the discrete volumes culture randomly resulting in a random combination of optically encoded particles in each well, or a unique combination of optically encoded particles may be specifically assigned to each discrete volume. The observable combination of optically encoded particles may then be used to identify each discrete volume.

Abstract: The subject matter disclosed herein is generally directed to methods and compositions for stable transduction of target cells with libraries of genetic elements. The invention reduces intermolecular recombination between library elements and integration of multiple genetic elements.

Abstract: The invention provides for use of CRISPR-Cas systems to sort barcoded cells or molecules. Cells or nucleic acid molecules may be sorted from a heterogenous population by targeting a barcode of interest specific for a cell or cell progeny or nucleic acid molecule of interest.

Abstract: Embodiments disclosed herein are directed to a new genetic perturbation and screening method that combines advantages of pooled perturbation with imaging assays for complex phenotypes. Specifically, the method may be used to screen pooled genomic perturbations to identify phenotypes and to identify perturbed genes at the single-cell level using optical barcodes. A major advantage offered by this approach is the ability to screen for any cellular phenotype that can be identified by high-resolution microscopy—including live-cell phenotypes, protein localization, or highly multiplexed expression profile and mRNA localization by RNA-FISH—in conjunction with a large array of genetic perturbations applied as a pool in a single test volume.

Abstract: This invention provides a cleaning and biocidal composition containing stabilized active bromine for treating hard surfaces contaminated with proteinaceous and fatty residues.

Abstract: A data analytics engine for facilitating real-time data analysis by respective subscriber based analytics processors is presented herein. An analytics engine component can generate even messages representing detected file-system events, e.g., comprising a creation, a modification, a read, a deletion, an open, a close, etc. of a file of a block device, a file system, etc. Further, the analytics engine component can store the event messages in a memory; receive defined notification criteria from a group of subscriber devices; and in response to determining that an event message of the event messages satisfies a defined notification criterion of the defined notification criteria corresponding to a subscriber device of the group of subscriber devices, send the event message directed to the subscriber device to facilitate an analysis of data corresponding to an access of the accesses of a file of the respective files.

Abstract: This invention is directed to a biocidal, antifouling or disinfection composition and methods of use thereof for inhibiting growth of microorganism and biofilms, reducing the microbial activity and killing microorganism in an aqueous system or on solid surfaces, wherein the biocidal composition includes stabilized chlorine compound such as dichloroisocyanurate salt, ammonium salt and a base.

Abstract: An aqueous biocidal composition comprising a mixture of hydrochloric acid (HCl), urea, and sodium hypochlorite (NaOCl) at acidic pH, a method for manufacturing the composition and a biocidal technology for treating industrial water.

Abstract: The invention provides a biocidal and anti-biofouling composition from highly concentrated precursors, and a process for manufacturing the composition.

Abstract: An exemplary system and method are disclosed for providing a combined video stream with geospatial information. Embodiments use a visualization tool to synchronize information in a virtual camera with attributes, such as video frames, from a physical camera. Once the camera position information is supplied to the visualization tool, the visualization tool retrieves geospatial data, such as terrain features, man made features, and time sensitive event data, and transforms the camera position information into a coordinate system of the virtual camera. The visualization tool uses the coordinate system of the virtual camera to generate geospatial snapshots. Embodiments use locative video software to combine the video frames from the physical camera with the geospatial snapshots from the virtual camera to generate a combined video stream, which provides a single view of multiple types of data to enhance an operator's ability to conduct surveillance and analysis missions.

Abstract: The invention provides a process of removing or preventing biofouling, particularly on surfaces of industrial and agricultural equipment in contact with aqueous liquids. The process employs an antifouling composition of stabilized active halogen having a low pH.

Abstract: Novel formulations containing a non-oxidative biocide, such as DBNPA, and a source of an in situ produced active biocide, such as a concentrated aqueous solution of an inorganic halide salt, are disclosed. These novel formulations are particularly effective in the treatment of water, and are characterized by high stability, desirable rheological properties and an excellent biocidal activity.