Patents by Inventor Dennis Flint

Dennis Flint has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Activity of Fe—S cluster requiring proteins
    Patent number: 10308964
    Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
    Type: Grant
    Filed: November 1, 2016
    Date of Patent: June 4, 2019
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Brian James Paul, Rick W. Ye
  • Fermentive production of four carbon alcohols
    Patent number: 9862976
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: January 9, 2018
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Activity of Fe-S Cluster Requiring Proteins
    Publication number: 20170101653
    Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
    Type: Application
    Filed: November 1, 2016
    Publication date: April 13, 2017
    Inventors: Dennis Flint, Brian James Paul, Rick W. Ye
  • Activity of Fe-S cluster requiring proteins
    Patent number: 9611482
    Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: April 4, 2017
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Brian James Paul, Rick W. Ye
  • Fermentive Production of Four Carbon Alcohols
    Publication number: 20170029851
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Application
    Filed: October 18, 2016
    Publication date: February 2, 2017
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Activity of Fe—S cluster requiring proteins
    Patent number: 9512435
    Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: December 6, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Brian James Paul, Rick W. Ye
  • Fermentive production of four carbon alcohols
    Patent number: 9506071
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: November 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof
    Publication number: 20160222370
    Abstract: Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous bacterial, fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
    Type: Application
    Filed: February 5, 2016
    Publication date: August 4, 2016
    Inventors: Larry Cameron ANTHONY, Dennis Flint, Wonchul Suh, Rick W. Ye, Steven Cary Rothman, Jean-Francois Tomb
  • Fermentive Production of Four Carbon Alcohols
    Publication number: 20160186186
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Application
    Filed: March 11, 2016
    Publication date: June 30, 2016
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Fermentive production of four carbon alcohols
    Patent number: 9365872
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: June 14, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Method for the production of isobutanol by recombinant yeast
    Patent number: 9303225
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: April 5, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Michael Charles Grady, Mehmedalija Jahic, Lori Ann Maggio-Hall, Vasantha Nagarajan, Ranjan Patnaik
  • Activity of Fe—S cluster requiring proteins
    Patent number: 9297016
    Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: March 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Brian James Paul, Rick W. Ye
  • Fermentive production of four carbon alcohols
    Patent number: 9297029
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: March 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS
    Publication number: 20150252389
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Application
    Filed: May 19, 2015
    Publication date: September 10, 2015
    Applicant: BUTAMAX ADVANCED BIOFUELS LLC
    Inventors: GAIL K. DONALDSON, ANDREW C. ELIOT, DENNIS FLINT, LORI ANN MAGGIO-HALL, VASANTHA NAGARAJAN
  • FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS
    Publication number: 20150247169
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Application
    Filed: May 19, 2015
    Publication date: September 3, 2015
    Applicant: Butamax Advanced Biofuels LLC
    Inventors: Gail K. DONALDSON, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Fermentive production of four carbon alcohols
    Patent number: 9068190
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: June 30, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Fermentive Production of Four Carbon Alcohols
    Publication number: 20150119608
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Application
    Filed: December 30, 2014
    Publication date: April 30, 2015
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases
    Patent number: 8951937
    Abstract: A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to ?-ketoisovalerate or 2,3-dihydroxymethylvalerate to ?-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 10, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Steven Cary Rothman, Wonchul Suh, Jean-Francois Tomb, Rick W. Ye
  • Fermentive production of four carbon alcohols
    Patent number: 8951774
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: November 4, 2010
    Date of Patent: February 10, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Production of isobutanol by a microorganism with enhanced acetolactate synthase activity
    Patent number: 8945859
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 3, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan