Patents by Inventor Douglas T. Gjerde

Douglas T. Gjerde has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method and device for extracting an analyte
    Patent number: 7943393
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.
    Type: Grant
    Filed: July 20, 2004
    Date of Patent: May 17, 2011
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Ronald Jones, Allen Burge
  • Method and Device for Extracting an Analyte
    Publication number: 20110042317
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides columns characterized by low backpressure.
    Type: Application
    Filed: October 28, 2010
    Publication date: February 24, 2011
    Inventors: Douglas T. Gjerde, Christopher P. Hanna
  • Method and Device for Sample Preparation
    Publication number: 20110038769
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
    Type: Application
    Filed: July 15, 2010
    Publication date: February 17, 2011
    Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
  • Open channel solid phase extraction systems and methods
    Patent number: 7879621
    Abstract: The invention provides, inter alia, methods, devices and reagents for the preparation of native and non-denatured biomolecules using solid-phase extraction channels. The invention is particularly suited for the purification, concentration and/or analysis of protein analytes. The invention further provides, inter alia, methods, devices and reagents for the purification, concentration and/or analysis of multi-protein complexes.
    Type: Grant
    Filed: December 10, 2003
    Date of Patent: February 1, 2011
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Christopher T. Hanna
  • Method and device for sample preparation
    Patent number: 7875462
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: January 25, 2011
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
  • Method and device for extracting an analyte
    Patent number: 7837871
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides columns characterized by low backpressure.
    Type: Grant
    Filed: August 17, 2004
    Date of Patent: November 23, 2010
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Christopher P. Hanna
  • METHOD AND DEVICE FOR SAMPLE PREPARATION
    Publication number: 20100200509
    Abstract: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
    Type: Application
    Filed: April 26, 2010
    Publication date: August 12, 2010
    Inventors: Chris Suh, Lee Hoang, Jennifer E. Grant, Douglas T. Gjerde
  • Method and Device for Sample Preparation
    Publication number: 20100179308
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
    Type: Application
    Filed: March 23, 2010
    Publication date: July 15, 2010
    Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
  • Method and Device for Gravity Flow Chromatography
    Publication number: 20100170852
    Abstract: The invention provides gravity chromatographic columns for the purification of a material (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the columns employ modified plates or racks as column bodies. In some embodiments, the invention provides methods and devices for gel filtration, desalting, buffer exchange, ion exchange, ion-pairing, normal phase and reverse phase chromatography. In some embodiments, the invention provides multiplexing gravity flow chromatography on a liquid handling robotic system.
    Type: Application
    Filed: March 22, 2010
    Publication date: July 8, 2010
    Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
  • Method and Device for Gravity Flow Chromatography
    Publication number: 20100140173
    Abstract: The invention provides gravity chromatographic columns for the automated purification of a material (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the columns employ modified plates or racks as column bodies. In some embodiments, the invention provides methods and devices for gel filtration, desalting, buffer exchange, ion exchange, ion-pairing, normal phase and reverse phase chromatography. In some embodiments, the invention provides multiplexing gravity flow chromatography on a liquid handling robotic system.
    Type: Application
    Filed: February 21, 2010
    Publication date: June 10, 2010
    Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
  • Highly Parallel Gel-Free Cloning Method
    Publication number: 20100129873
    Abstract: A highly parallel method for gene cloning is presented. PCR products can be isolated using a solid phase and ligated into a positive selection vector. The cloning method has a very high success rate and can be performed entirely by a liquid handling robot with very little human intervention.
    Type: Application
    Filed: November 24, 2009
    Publication date: May 27, 2010
    Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde, David P. Hornby
  • Method and device for sample preparation
    Patent number: 7722820
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.
    Type: Grant
    Filed: November 21, 2005
    Date of Patent: May 25, 2010
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
  • Open Channel Solid Phase Extraction Systems and Methods
    Publication number: 20100111771
    Abstract: The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. In some embodiments, the analyte is a protein. The invention further provides reagents, channels, columns and instrumentation related to this and other methods.
    Type: Application
    Filed: January 11, 2010
    Publication date: May 6, 2010
    Inventors: Douglas T. Gjerde, Christopher P. Hanna
  • Pipette Tip Containing Particle-Filled Polymer Monolith
    Publication number: 20100009845
    Abstract: The present invention relates to a pipette tip which is fitted with a porous organic monolith which is doped with active particles. Due to a unique polymerization method, the extraction tips stay highly permeable which allows sample to pass through the monolithic bed. The extraction tip represents an ideal tool for solid phase extraction, especially for desalting, isolating and purifying biomolecules such as peptides and proteins.
    Type: Application
    Filed: June 5, 2009
    Publication date: January 14, 2010
    Inventors: Alexander Bonn, Guenther Bonn, Christian Huck, Bernhard Maerk, Harald Sonderegger, Mathias Rainer, Douglas T. Gjerde
  • Solid-phase synthesis is a capillary
    Patent number: 7612165
    Abstract: The invention provides, inter alia, methods for synthesizing a molecule on the channel surface of a capillary, comprising the steps of: (i) covalently attaching a first chemical entity to the channel surface of a capillary; and (ii) covalently attaching a second chemical entity to the first chemical entity, wherein the covalent attachment steps are part of a process for synthesizing a molecule on the channel surface.
    Type: Grant
    Filed: February 24, 2006
    Date of Patent: November 3, 2009
    Assignee: PhyNexus, Inc.
    Inventor: Douglas T. Gjerde
  • Method and Device for Desalting an Analyte
    Publication number: 20090223893
    Abstract: The invention provides gel filtration columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of gel filtration media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods and devices for desalting and/or buffer exchange of a sample.
    Type: Application
    Filed: May 4, 2009
    Publication date: September 10, 2009
    Inventors: Douglas T. Gjerde, Lee Hoang, Chris Suh, Mark W. Abel
  • Low Dead Volume Extraction Column Device
    Publication number: 20090081084
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.
    Type: Application
    Filed: December 5, 2008
    Publication date: March 26, 2009
    Inventors: Douglas T. Gjerde, Christopher T. Hanna
  • Method and device for extracting an analyte
    Patent number: 7488603
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the elution of analyte in a small volume of liquid.
    Type: Grant
    Filed: August 17, 2004
    Date of Patent: February 10, 2009
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Christopher P. Hanna
  • Low dead volume extraction column device
    Patent number: 7482169
    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.
    Type: Grant
    Filed: July 14, 2003
    Date of Patent: January 27, 2009
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Christopher T. Hanna
  • Method and device for extracting an analyte
    Publication number: 20080214794
    Abstract: The invention provides columns and methods for the purification and concentration of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution. The columns typically include a bed of extraction medium positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for purifying and concentrating multiple analytes simultaneously.
    Type: Application
    Filed: December 21, 2007
    Publication date: September 4, 2008
    Inventor: Douglas T. Gjerde