Abstract: The invention concerns the field of cell culture technology. It concerns a method of improving cell growth, especially the growth of biopharmaceutical producer host cells. The invention further concerns a method of producing proteins using the cells generated by the described method.

Abstract: The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpper® Discovery) and DSM ACC2910 (CHOpper® Standard). The invention further concerns a method of producing proteins using the cells generated by the described method.

Abstract: Apparatus, systems, and methods disclosed herein may initialize a delay-locked loop (DLL) or phase-locked loop (PLL) to achieve a locked condition and may then initiate a quiescent mode of operation. Quiescent operation may be achieved by breaking a feedback loop associated with the DLL or PLL to prevent updates to a variable delay line associated with the DLL and/or to a variable frequency oscillator associated with the PLL. An output clock phase associated with the DLL or PLL may thus be held substantially constant following a DLL initialization period. Additional embodiments are disclosed and claimed.

Abstract: The present invention concerns the field of cell culture technology. It describes a novel method for enhancing the secretory transport of proteins in eukaryotic cells by heterologous expression of Munc18c, Sly1 or other members of the SM protein family. This method is particularly useful for the generation of optimized host cell systems with enhanced production capacity for the expression and manufacture of recombinant protein products.

Abstract: Closed-loop duty-cycle correctors (DCCs), clock generators, memory devices, systems, and methods for generating an output clock signal having a particular duty cycle are provided, such as clock generators configured to generate an output clock signal synchronized with a received input clock signal having a predetermined duty cycle. Embodiments of clock generators include closed-loop duty cycle correctors that receive an already-controlled and corrected output signal. For example, DLL control circuitry and DCC control circuitry may each adjust a delay of a variable delay line. The DLL control circuitry adjusts the delay such that an output clock signal is synchronized with an input clock signal. The DCC control circuitry detects a duty cycle error in the output clock signal and adjusts the delay of the variable delay line to achieve a duty cycle corrected output signal.

Abstract: A method and apparatus for cleaning printed circuit boards are provided. The method includes providing a cleaning apparatus with a housing having a conveyance mechanism for carrying printed circuit boards through the housing. The cleaning apparatus has at least a prewash station, a wash station and a final rinse station therein. The printed circuit boards are carried on the conveyance mechanism to the prewash station. A plurality of fluidic oscillator nozzles of the prewash station are utilized to direct liquid onto the printed circuit boards. Each fluidic oscillator nozzle outputs a stream of liquid with an instantaneous direction that oscillates back and forth relative to a nozzle axis over time.

Abstract: The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.

Abstract: An apparatus and method are disclosed for retaining an attachment, such as an awning, to the wall of a recreational vehicle. The apparatus includes a flexible strap assembled from a plurality of strap segments. Male and female portions of adjacent strap segments are joined together to form a flexible interconnection between adjacent strap segments. The apparatus also includes an upper mounting portion for fixedly connecting a first end of the strap to the wall, and a lower mounting portion for detachably connecting a second end of the strap to the wall. Also disclosed are methods of installing and using an apparatus for retaining a recreational vehicle attachment.

Abstract: The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.

Abstract: An apparatus and method are disclosed for retaining an attachment, such as an awning, to the wall of a recreational vehicle. The apparatus includes a flexible strap assembled from a plurality of strap segments. Male and female portions of adjacent strap segments are joined together to form a flexible interconnection between adjacent strap segments. The apparatus also includes an upper mounting portion for fixedly connecting a first end of the strap to the wall, and a lower mounting portion for detachably connecting a second end of the strap to the wall. Also disclosed are methods of installing and using an apparatus for retaining a recreational vehicle attachment.

Abstract: A delay locked loop circuit and method of operating the same. The delay locked loop circuit comprises a forward delay path having a variable delay portion and a static delay portion, wherein the static delay portion includes a static delay element, a feedback path for generating a feedback signal responsive to an output signal, and a phase detector for comparing the phase of an input signal and the phase of the feedback signal and for generating a variable control signal for controlling the amount of delay provided by the variable delay portion, wherein the static delay element is activated in response to a static control signal indicative of the variable delay portion being unable to lock the output signal to the input signal. Because of the rules governing Abstracts, this Abstract should not be used to construe the claims.

Abstract: A system and method for the automated provisioning of software applications onto one or more computers. The provisioning system uses a model of the application, which describes the application in terms of its software features and the actions to manipulate the application. The model preferably describes this information in a general way, such that it is independent of any environmental characteristics associated with the computers. The provisioning system also receives as input a set of references to selected ones of the computers onto which the application is to be provisioned.

Abstract: A delay locked loop circuit and method of operating the same. The delay locked loop circuit comprises a forward delay path having a variable delay portion and a static delay portion, wherein the static delay portion includes a static delay element, a feedback path for generating a feedback signal responsive to an output signal, and a phase detector for comparing the phase of an input signal and the phase of the feedback signal and for generating a variable control signal for controlling the amount of delay provided by the variable delay portion, wherein the static delay element is activated in response to a static control signal indicative of the variable delay portion being unable to lock the output signal to the input signal. Because of the rules governing Abstracts, this Abstract should not be used to construe the claims.

Abstract: A delay locked loop circuit and method of operating the same. The delay locked loop circuit comprises a forward delay path having a variable delay portion and a static delay portion, wherein the static delay portion includes a static delay element, a feedback path for generating a feedback signal responsive to an output signal, and a phase detector for comparing the phase of an input signal and the phase of the feedback signal and for generating a variable control signal for controlling the amount of delay provided by the variable delay portion, wherein the static delay element is activated in response to a static control signal indicative of the variable delay portion being unable to lock the output signal to the input signal. Because of the rules governing Abstracts, this Abstract should not be used to construe the claims.

Abstract: A delay locked loop circuit and method of operating the same. The delay locked loop circuit comprises a forward delay path having a variable delay portion and a static delay portion, wherein the static delay portion includes a static delay element, a feedback path for generating a feedback signal responsive to an output signal, and a phase detector for comparing the phase of an input signal and the phase of the feedback signal and for generating a variable control signal for controlling the amount of delay provided by the variable delay portion, wherein the static delay element is activated in response to a static control signal indicative of the variable delay portion being unable to lock the output signal to the input signal. Because of the rules governing Abstracts, this Abstract should not be used to construe the claims.

Abstract: A system and method for the management of applications on one or more computers. The management system can manage the applications based on the state of each resource belonging to the application. One aspect of the management system includes a master server and a plurality of agents. The master server allows resources to be selected from among a plurality of resources that are already deployed and combine the resources together to form an application. The master server can then generate instructions for capturing the selected resources, along with their corresponding resource states, and combining the captured resources and resource states into an application package. Another aspect of the management system can associate management procedures with the application based on the resource type of each of the resources making up the application. The management procedures can thus be executed to manage the application based on the states of each of its resources.

Abstract: A system and method for the automated provisioning of software applications onto one or more computers. The provisioning system uses a model of the application, which describes the application in terms of its software features and the actions to manipulate the application. The model preferably describes this information in a general way, such that it is independent of any environmental characteristics associated with the computers. The provisioning system also receives as input a set of references to selected ones of the computers onto which the application is to be provisioned.