Patents by Inventor Frank Buchholz
Frank Buchholz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11845954Abstract: The invention provides methods for specifically altering the DNA sequence in a genome, in particular for genome editing by deleting or replacing a sequence of interest. Advantageously, the invention uses two non-identical sequences naturally occurring in a genome as target sites two which DNA-recombining enzymes are generated. The invention is in particular useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (indels).Type: GrantFiled: June 14, 2018Date of Patent: December 19, 2023Assignee: TECHNISCHE UNIVERSITÄT DRESDENInventors: Frank Buchholz, Martin Schneider, Felix Lansing
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Publication number: 20230235364Abstract: The invention provides methods and means for specifically altering the DNA sequence in a genome, in particular for genome editing by deleting or replacing a sequence of interest. Advantageously, the invention uses two non-identical sequences naturally occurring in a genome as target sites two which DNA-recombining enzymes are generated. The invention is in particular useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (in-dels).Type: ApplicationFiled: October 12, 2022Publication date: July 27, 2023Inventors: Frank BUCHHOLZ, Martin SCHNEIDER, Felix LANSING
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Publication number: 20230183713Abstract: The invention relates to the field of genome editing and provides a method of generating DNA recombinases or provides DNA recombinases, which efficiently and specifically recombine genomic target sequences as obligate DNA recombinase enzymes.Type: ApplicationFiled: November 15, 2022Publication date: June 15, 2023Inventors: Jenna HOERSTEN, Felix LANSING, Frank BUCHHOLZ
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Publication number: 20210147878Abstract: The invention provides methods and means for specifically altering the DNA sequence in a genome, in particular for genome editing by deleting or replacing a sequence of interest. Advantageously, the invention uses two non-identical sequences naturally occurring in a genome as target sites two which DNA-recombining enzymes are generated. The invention is in particular useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (indels).Type: ApplicationFiled: June 14, 2018Publication date: May 20, 2021Applicant: TECHNISCHE UNIVERSITÄT DRESDENInventors: Frank BUCHHOLZ, Martin SCHNEIDER, Felix LANSING
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Patent number: 10316301Abstract: The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.Type: GrantFiled: November 10, 2016Date of Patent: June 11, 2019Assignees: Heinrich-Pette-Institut, Leibniz-Institut für Experimentelle Virologie, Max-Planck-Gesellschaft Zur Förderung der Wissenschaften E.V.Inventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Chusainow
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Patent number: 10253332Abstract: The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.Type: GrantFiled: June 27, 2017Date of Patent: April 9, 2019Assignee: TECHNISCHE UNIVERSITAT DRESDENInventors: Frank Buchholz, Madina Karimova
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Patent number: 10150953Abstract: The present invention relates to a method for preparing an expression vector encoding a well-tolerated and highly specific tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains which may be inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with these, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection, in particular, HIV infection. In particular, the invention relates to well-tolerated and highly specific tailored recombinases capable of combining asymmetric target sequences in a more than 90% of HIV-strains, thereby excising the HIV-1 sequences, and expression vectors encoding them.Type: GrantFiled: September 1, 2015Date of Patent: December 11, 2018Assignees: Heinrich-Pette-Institut Leibniz-Institut für Experimentelle Virologie-Stiftung bürgerlichen Rechts, Technische Universität DresdenInventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Karpinski
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Publication number: 20170306356Abstract: The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.Type: ApplicationFiled: June 27, 2017Publication date: October 26, 2017Inventors: Frank BUCHHOLZ, Madina KARIMOVA
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Publication number: 20170254402Abstract: The invention relates to a wave generator for a strain wave gear, said wave generator in a main portion having a bearing seat for a radially flexible roller bearing. The wave generator is distinguished by having a cone portion that adjoins the main portion in the axial direction and tapers off in a direction away from the main portion.Type: ApplicationFiled: March 3, 2017Publication date: September 7, 2017Inventors: Siegmar GILGES, Tim Frank BUCHHOLZ
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Patent number: 9719109Abstract: The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.Type: GrantFiled: July 21, 2016Date of Patent: August 1, 2017Assignee: Technische Universitat DresdenInventors: Frank Buchholz, Madina Karimova
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Publication number: 20170175091Abstract: The present invention relates to a method for preparing an expression vector encoding a well-tolerated and highly specific tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains which may be inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with these, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection, in particular, HIV infection. In particular, the invention relates to well-tolerated and highly specific tailored recombinases capable of combining asymmetric target sequences in a more than 90% of HIV-strains, thereby excising the HIV-1 sequences, and expression vectors encoding them.Type: ApplicationFiled: September 1, 2015Publication date: June 22, 2017Inventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Karpinski
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Publication number: 20170130212Abstract: The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.Type: ApplicationFiled: November 10, 2016Publication date: May 11, 2017Inventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Chusainow
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Publication number: 20170016029Abstract: The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.Type: ApplicationFiled: July 21, 2016Publication date: January 19, 2017Inventors: Frank BUCHHOLZ, Madina KARIMOVA
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Patent number: 9428754Abstract: The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.Type: GrantFiled: July 22, 2013Date of Patent: August 30, 2016Assignee: Technische Universität DresdenInventors: Frank Buchholz, Madina Karimova
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Publication number: 20150176012Abstract: The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.Type: ApplicationFiled: July 22, 2013Publication date: June 25, 2015Inventors: Frank Buchholz, Madina Karimova
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Publication number: 20150104433Abstract: The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.Type: ApplicationFiled: December 4, 2014Publication date: April 16, 2015Inventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Chusainow
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Patent number: 8871516Abstract: The present invention is directed to a method for preparing an expression vector encoding a tailored recombinase, wherein said tailored recombinase recombines asymmetric target sites within the LTR of proviral DNA of a retrovirus inserted into the genome of a host cell and is useful as means for excising the provirus from the genome of the host cell. The present invention further relates to an in vitro-method of optimising the treatment of a retroviral infection of a subject and to the use of tailored recombinases for the preparation of pharmaceutical compositions for reducing the viral load in a subjected infected by a retrovirus.Type: GrantFiled: January 3, 2008Date of Patent: October 28, 2014Assignees: Technische Universität Dresden, Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V., Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität HamburgInventors: Joachim Hauber, Frank Buchholz, Ilona Hauber, Francis A Stewart, Indrani Sarkar
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Patent number: 8819155Abstract: Methods, systems, and apparatuses for processing function calls using a hooking routines to perform pre-execution tasks are disclosed. A server computer receives a function call including a hooking routine and a request to run a particular function from a client computer. Based on a function group identifier and the credentials of the user or client computer, the server computer can perform various pre-execution tasks. The tasks can be common to all functions in the function group or be customized Both pre-execution tasks can be stored remotely from the client computer, so that updates to the pre-execution task can be made without the need to update computer readable code stored on the client computer. If the pre-execution tasks, such as an authorization, passes, then the server computer can execute the requested function, the server computer can reject or abort the requested function.Type: GrantFiled: December 16, 2011Date of Patent: August 26, 2014Assignee: Business Objects Software LimitedInventors: Alexander Ocher, Frank Buchholz, Werner Daehn, Wu Cao, Ben Hofmans
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Publication number: 20130303594Abstract: The invention relates to polynucleotides and polypeptides as defined in the claims for use in the treatment of a disorder associated with abnormal telomerase activity and/or abnormal telomere length in comparison to healthy subjects in a subject. Moreover, the present invention provides a method of identifying an agent capable of decreasing telomerase activity and/or telomere length, and a method of classifying a cancer as a telomerase-negative cancer, as defined in the claims.Type: ApplicationFiled: September 14, 2011Publication date: November 14, 2013Applicant: Max-Planok-Gesellschaft Zur Forderung Der Wissenschaften E.V.Inventors: Dennis Kappei, Frank Buchholz, Matthias Mann, Falk Butter
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Publication number: 20130164271Abstract: The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.Type: ApplicationFiled: May 27, 2011Publication date: June 27, 2013Applicants: MAX-PLANCK-GESELLSCHAFT ZUR FOEDERUG DER WISSENSCH E.V., HENRICH-PETTE-INSTITUT LEIBNZ-INSTITUT FOR EXPERIM VIROLOGIE-STIFTUNG BInventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Chusainow