Abstract: The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds.

Abstract: The present invention relates to a chromatography ligand defined by the following formula (I) R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

Abstract: The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.

Abstract: The invention relates to an aqueous dispersion of cellulose-reactive sizing agent containing an acid anhydride, an anionic polyelectrolyte and a nitrogen-containing organic compound which is an amine or quaternary ammonium thereof having a molecular weight less than 180 and/or having one or more hydroxyl groups. The invention further relates to a process for the production of paper which comprises adding the aqueous dispersion of cellulose-reactive sizing agent to an aqueous cellulosic suspension.

Abstract: The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.

Abstract: The present invention refers to a process for the production of paper from a suspension containing cellulosic fibers, and optionally fillers, comprising adding to the suspension at least one cationic organic polymer and an aqueous silica-containing composition comprising an anionic naphthalene sulphonate formaldehyde condensate and anionic silica-based particles, the composition having a weight ratio of naphthalene sulphonate formaldehyde condensate to silica-based particles within the range of from 0.2:1 to 99:1, and containing naphthalene sulphonate formaldehyde condensate and silica-based particles in an amount of at least 0.01% by weight, based on the total weight of the aqueous silica-containing composition, and with the proviso that the composition contains substantially no cellulose-reactive sizing agent. The invention also encompasses an aqueous silica-containing composition and a method for preparation of an aqueous silica-containing compound.

Abstract: The present invention relates to a heating device having at least a first heating element 102 for heating at least one protective sleeve 105, 602 arranged around at least a first uncoated optical fiber section 501, 603. Said sleeve 105, 602 being arranged to shrink when exposed to heat to form a protecting member tightly enclosing said uncoated optical fiber section 501, 603. The at least first heating element is flexible, and arranged to take at least a first open state and a second substantially closed state. Said first open state is suitable for inserting and removing said protecting sleeve and fiber into and out off said heating device. Said heating element, in said second state, substantially surrounds said sleeve to form an enclosure around said sleeve, so that said heating element radiates heat around substantially the complete circumference of said sleeve.

Abstract: In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore. In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs. In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher.

Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

Abstract: The present invention relates to the use of an alkali-stable protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

Abstract: The present invention relates to a separation matrix comprised of porous particles to which antibody-binding protein ligands have been immobilised, wherein the ligand density is in the range of 5.0-10 mg/ml; the gel phase distribution coefficient of the particles expressed as Kav for a dextran of size 110 kDa is above 0.65 and the median particle diameter is between 65-84 ?m. The carbohydrate material is preferably highly cross-linked agarose.

Abstract: The invention relates to an apparatus and a method for marshalling out individual objects (1) from a row of such objects (1). The apparatus includes two pusher arms (8) placed one on each side of the row of objects (1). The apparatus also includes two arrest pins (20, 21) on either side of the row, one front (20) and one rear (21). The apparatus further includes a linkage (13) on each side of the row, axially connected to a pusher arm (8) and interconnected with each other via a spring (14), respectively. The pusher arms (8) are moved, in a closed position, forwards with the aid of a piston and cylinder assembly (19) and, in this movement, marshal out the first object (1) in the row. Thereafter, the pusher arms (8) open in that they enter into abutment against the front arrest pin (20). The linkage (13) ensures that the pusher arms (8) assume an open position. The pusher arms (8) thereafter move backwards in the open position with the aid of the piston and cylinder assembly (19).

Abstract: The present disclosure describes a packaging laminate where at least one of the material layers included in the laminate includes magnetisable particles. Further, a roller is disclosed which includes means for applying crease line patterns on a packaging laminate and means for applying magnetic fields for magnetising magnetisable particles in the packaging laminate. Finally, a layer is also described for use for a packaging laminate, the layer including magnetisable particles.

Abstract: The invention related to process for the production of paper from an aqueous suspension containing cellulosic fibers, and optional fillers, which comprises adding to the suspension (i) a cationic vinyl addition polymer prepared by polymerizing one or more ethylenically unsaturated monomers comprising a cationic monomer; (ii) an anionic polymer having one or more aromatic groups selected from the group consisting of polymers obtained by condensation of formaldehyde with benzene-based and naphthalene-based compounds, polyurethanes, polysaccharides, and lignin-based polymers; wherein (i) and (ii) are separately added to the suspension; and (iii) a low molecular weight cationic organic polymer having a molecular weight in the range of from 2,000 to about 700,000; or an anionic microparticulate material; and forming and draining the obtained suspension on a wire.

Abstract: A packaging laminate (10) comprising a core layer (11) and a gas barrier layer (14) of moisture- and liquid-sensitive barrier polymer, e.g. a copolymer of ethylene and vinyl alcohol (EVOH). The packaging laminate (10) preferably has outer liquid-tight coatings (12 and 13) of thermoplastic in order to make for sealing of the packaging laminate by thermosealing. In order to impart increased moisture and liquid protection to the moisture- and liquid-sensitive barrier layer (14), the packaging laminate (10) has a layer (16) of mineral filled polyolefin between the barrier layer (14) and the outer, liquid-tight coating (13) on the one side of the core layer (11). A packaging container produced from the packaging laminate (10) makes for the storage of a packed liquid food, such as juice, wine, cooling oil etc., with so-called extended shelf life.

Abstract: The present invention refers to a process for the production of paper from a suspension containing cellulosic fibres, and optionally fillers, comprising adding to the suspension at least one cationic organic polymer and an aqueous silica-containing composition comprising an anionic naphthalene sulphonate formaldehyde condensate and anionic silica-based particles, the composition having a weight ratio of naphthalene sulphonate formaldehyde condensate to silica-based particles within the range of from 0.2:1 to 99:1, and containing naphthalene sulphonate formaldehyde condensate and silica-based particles in an amount of at least 0.01% by weight, based on the total weight of the aqueous silica-containing composition, and with the proviso that the composition contains substantially no cellulose-reactive sizing agent. The invention also encompasses an aqueous silica-containing composition and a method for preparation of an aqueous silica-containing compound.

Abstract: A process for the production of an aqueous sol containing silica-based particles which comprises (a) acidifying an aqueous silicate solution to a pH of from 1 to 4 to form an acid sol; (b) alkalising the acid sol at an SiO2 content within the range of from 4.5 to 8% by weight; (c) allowing particle growth of the alkalised sol for at least 10 minutes; or heat-treating the alkalised sol at a temperature of a least 30° C.; (d) alkalising the obtained sol to a pH of at least 10.0; and (e) optionally concentrating the sol obtained according to (b), (c) or (d) to provide an aqueous sol containing silica-based particles and having a specific surface area of at least 90 m2/g aqueous sol; as well as an aqueous sol containing silica-based particles obtainable by the process.

Abstract: The present invention relates to an aqueous silica-containing composition comprising an anionic organic polymer having at least one aromatic group and anionic silica-based particles. The aqueous silica-containing composition contains the anionic organic polymer having at least one aromatic group and the anionic silica-based particles in an amount of at least 0.01% by weight of the total weight of the aqueous silica-containing composition. The aqueous silica-containing composition contains substantially no cellulose-reactive sizing agent and the anionic organic polymer having at least one aromatic group is not an anionic naphthalene sulphonate formaldehyde condensate. The invention further relates to methods for the preparation of the aqueous silica-containing composition and the use of the aqueous silica-composition as a drainage and retention aid in a process for the production of paper.

Abstract: The present invention relates to a heating device having at least a first heating element 102 for heating at least one protective sleeve 105, 602 arranged around at least a first uncoated optical fiber section 501, 603. Said sleeve 105, 602 being arranged to shrink when exposed to heat to form a protecting member tightly enclosing said uncoated optical fiber section 501, 603. The at least first heating element is flexible, and arranged to take at least a first open state and a second substantially closed state. Said first open state is suitable for inserting and removing said protecting sleeve and fiber into and out off said heating device. Said heating element, in said second state, substantially surrounds said sleeve to form an enclosure around said sleeve, so that said heating element radiates heat around substantially the complete circumference of said sleeve.

Abstract: The disclosure relates to a packaging laminate including a rigidifying core which comprises two rigid outer layers of which one of these two layers consists of a layer of mineral-filled polyolefin, as well as a bulking layer disposed between the above mentioned two rigid outer layers. According to the present invention, the bulking layer consists of a non-woven material. The present invention also relates to a packaging container preferably for liquid foods which is produced by fold forming and sealing of the sheet or web-shaped packaging laminate.