Abstract: The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, including a duct (5) for delivering a coolant (3) to a cooling chamber (1), a heater (6) that has an adjustable first heating performance (P2) for heating the coolant (3) delivered to the cooling chamber (1), a first temperature sensor (8-10) for measuring the temperature (T2-T4) in the cooling chamber (1), a second temperature sensor (7) for measuring the temperature (Ti) of the coolant (3) delivered to the cooling chamber (I), and a regulator (11) for regulating the temperature. The regulator (11) is embodied as a multiple regulator which detects several temperatures (T1-T4) as control variables and/or adjusts several heating performances (P1, P2) as manipulated variables. The invention further relates to a corresponding operating method.

Abstract: A sample support (100, 101, 102, . . . ), in particular for cryopreservation of biological samples, is described and includes: a holder part (10) with a sample receiving chamber (11), a retaining device (12, 13) on which a sample can be disposed, and support connecting elements (14, 15) which are directed at opposite holder part ends (16, 17) and are formed to fit together so that a plurality of holder parts (16) can be put together. A sample store (200), which includes an assemblage of a large number of such sample supports, and a method for the cryopreservation of biological samples, are also described.

Abstract: The invention relates to an encapsulating device (100), which is designed to encapsulate a sample (1, 2) in a polymer capsule, comprising a drop generator (10), which is designed to provide a drop (3) of a suspension, which drop contains the sample (1), and a cross-linking device (20), which is designed to polymerize the drop (3), wherein the drop generator (10) has a retaining device (11), which is designed to accommodate the drop (3) in a hanging state, and the cross-linking device (20) is designed to feed a polymerization substance to the hanging drop (3) on the retaining device (11) and to form the polymer capsule. The invention further relates to a method for encapsulating a sample (1, 2) in a polymer capsule.

Abstract: A protective suit (100), especially for an operator (1) in a cooling chamber that is cooled using liquid nitrogen or vapor of the liquid nitrogen comprises a body suit (10) which has a thermally insulating, gas-tight cover material (20) and is designed to accommodate the operator (1), and a heating device (30) which is connected to the body suit (10) and is designed to heat the interior of the protective suit (100). A glove (70) which is made of a thermally insulating glove material (71) and includes a glove heater (77) is also described.

Abstract: A culture vessel (100), which is designed for culturing biological cells (1) in hanging drops (2), comprises a vessel wall (10), which has a cover portion (11) and a floor portion (12), wherein the cover portion (11) is designed to provide a culturing surface (13), and the floor portion (12) is designed to receive a liquid (3), the vessel wall (10) encloses an interior (20) of the culture vessel on all sides, the culturing surface (13) has holding elements (14), which are designed to position the drops (2), wherein the culturing surface (13) can receive the drops (2) hanging freely in the interior (20), and the vessel wall (10) is movable such that the holding elements (14) can be wetted with the liquid (3) from the floor portion (12). A method for culturing biological cells (1) in hanging drops (2) in the culture vessel (100) is also described.

Abstract: A cooling system (1), especially for cryopreserving biological samples (2), comprises a cooling chamber (100) delimited by a bottom area (110), side walls (120), and a top area (130), and a cooling device (200) for cooling the cooling chamber (100) using liquid nitrogen (220). At least one of the side walls (120) includes at least one predetermined wall element (125) which is a portion of at least one of the side walls and can be moved relative to the associated side wall (120) in such a way that a wall opening can be formed in said side wall (120). Methods for operating the cooling system (1) in case of an emergency are also described.

Abstract: The invention relates to a cryogenic storage device (100), in particular for storing biological samples (1) in the cryopreserved state, comprising a storage container (cryogenic tank 10) for cooling the samples in a reservoir (12) of liquid nitrogen or in a nitrogen vapour above the reservoir, a hood (21), and a flushing device (30) for introduction of a coolant (preferably cold nitrogen gas) into the hood space (22). The storage container is closed by a lid section (13). The coolant conduit (31) of the flushing device opens in the hood space (22). The storage temperature is adjustable in a locally delimited cooling section (24) by the refrigerant stream (2). The flushing device comprises a coolant vessel (32), which is formed by the storage container (10) with the reservoir of liquid nitrogen and/or an additional container. The invention further relates to a method for operating the cryogenic storage device.

Abstract: Disclosed is a deposition apparatus (100) which is designed to deposit frozen biological material (1) in a target substrate (2) and includes a charging device (10) and a driving device (20). The charging device (10) is designed to supply the biological material (1) to the driving device (20) while the driving device (20) is designed to apply a driving force to the biological material (1). In order to apply the driving force, the driving device (20) is formed such that the biological material (1) can penetrate into the target substrate (2) under the effect of the driving force. Also disclosed are an injector cartridge which is designed to provide frozen biological material in a deposition apparatus as well as a method for depositing biological material (1) in a target substrate (2).

Abstract: The invention relates to a method for lifting an object (1) from the sea floor, comprising the steps: coupling of the object (1) to a buoyancy balloon (10), and a buoyant motion of said buoyancy balloon (10) with the object (1), the buoyancy balloon (10) being filled with a buoyancy liquid such as, for example, water, the temperature of which is higher than the temperature of the sea water surrounding said buoyancy balloon (10). The invention also relates to a balloon device (100) which is configured for lifting an object (1) from the sea floor.

Abstract: The invention relates to a substrate unit (100), in particular for the cryopreservation of a biological sample (1) containing biological cells (2, 2.1, 2.

Abstract: A cryopreservation storage device (100), in particular for cryopreservation of biological samples, comprises a plurality of multi sample modules (20) being adapted for accommodating the biological samples and sample memories, a module control device (30) controlling an access to sample memories accommodated by the multi sample modules (20), and a data interface (41) for accessing to the module control device (30), wherein the module control device (30) includes a data management processor (31), which can be controlled via the data interface (41). Furthermore, a cryopreservation apparatus including at least one cryopreservation storage device (100) and a method for cryopreservation of biological samples are described.

Abstract: The invention relates to an aquarium with a pressure-tight aquarium tank, which during operation is filled with a liquid, and with a pressure-resistant rising pipe, which opens out into the aquarium tank and is intended for producing an increased hydrostatic pressure in the aquarium tank by a liquid column located in the rising pipe.

Abstract: The invention relates to a cryogenic storage device and to a method for operating a cryogenic storage device, particularly for cryogenic storage of biological samples, comprising a sample carrying device that is set up to accommodate samples, a cryogenic container that is set up to accommodate the sample carrying device, a container cooling device with which the cryogenic container can be cooled, an intermediate storage container that is set up for intermediate storage of the sample carrying device, a transport device with which the sample carrying device can be moved between the cryogenic container and the intermediate storage container and a sensor device with which at least one operating parameter of the cryogenic storage device can be registered.

Abstract: A cryopreservation device (100) which is arranged for cryopreservation of a biological sample (1) comprises a pressure vessel (10) with a vessel wall (11) and an internal space (12) which is arranged to receive the biological sample (1), wherein the pressure vessel (10) is equipped with an actuating device (20) for cooling by lowering the temperature and raising the pressure in the pressure vessel (10) and configured for the cryopreservation of the biological sample (1). Said actuating device (20) is connected to the vessel wall (11) and comprises at least one pressure setting element (21-23, 31) and at least one of at least one cooling element (34) and at least one heat conducting element (35-38), wherein the actuating device (20) is configured for a time-dependent and/or location-dependent setting of the temperature and of the pressure in the pressure vessel (10).

Abstract: The invention relates to a method for conserving at least one biological deep-sea organism (1) comprising the steps of collecting the biological organism (1) in a deep-sea trapping device (110); cooling the biological organism (1) with a cooling device (120), wherein the biological organism (1) is put in a frozen condition; and moving the frozen biological organism (1), preferably with the trapping device (110), to a normal-pressure environment. The invention further relates to a conservation apparatus (100) for conserving at least one biological deep-sea organism (1).

Abstract: An adapter device includes a receptacle including at least one receiving element that detachably secures a sample chamber for cryoconservation of a biological sample, and a coupling device that connects the receptacle to a base section which can be secured in a cooling device, wherein the receiving element is 1) immobile in a holding state at a cryoconservation temperature and secures the sample chamber and 2) at least one of deformable and movable in a release state at a temperature above the cryoconservation temperature so that the sample chamber is detachable from the adapter device or can be inserted into the adapter device.

Abstract: The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, including a duct (5) for delivering a coolant (3) to a cooling chamber (1), a heater (6) that has an adjustable first heating performance (P2) for heating the coolant (3) delivered to the cooling chamber (1), a first temperature sensor (8-10) for measuring the temperature (T2-T4) in the cooling chamber (1), a second temperature sensor (7) for measuring the temperature (Ti) of the coolant (3) delivered to the cooling chamber (I), and a regulator (11) for regulating the temperature. The regulator (11) is embodied as a multiple regulator which detects several temperatures (T1-T4) as control variables and/or adjusts several heating performances (P1, P2) as manipulated variables. The invention further relates to a corresponding operating method.

Abstract: A cooling system (1), especially for cryopreserving biological samples (2), comprises a cooling chamber (100) delimited by a bottom area (110), side walls (120), and a top area (130), and a cooling device (200) for cooling the cooling chamber (100) using liquid nitrogen (220). The bottom area (110) is designed for direct cooling using the liquid nitrogen, and the cooling chamber (100) is dimensioned such that an operator (3) can access and move around in the cooling chamber (100). The bottom area (110) has a platform (111) which is permeable to vapor of the liquid nitrogen (220) and forms a support zone for the operator (3). Methods for operating the cooling system are also described.

Abstract: A cooling system (1), especially for cryopreserving biological samples (2), comprises a cooling chamber (100) delimited by a bottom area (110), side walls (120), and a top area (130), and a cooling device (200) for cooling the cooling chamber (100) using liquid nitrogen (220). At least one of the side walls (120) includes at least one predetermined wall element (125) which is a portion of at least one of the side walls and can be moved relative to the associated side wall (120) in such a way that a wall opening can be formed in said side wall (120). Methods for operating the cooling system (1) in case of an emergency are also described.

Abstract: A protective suit (100), especially for an operator (1) in a cooling chamber that is cooled using liquid nitrogen or vapor of the liquid nitrogen comprises a body suit (10) which has a thermally insulating, gas-tight cover material (20) and is designed to accommodate the operator (1), and a heating device (30) which is connected to the body suit (10) and is designed to heat the interior of the protective suit (100). A glove (70) which is made of a thermally insulating glove material (71) and includes a glove heater (77) is also described.