Abstract: The present invention provides an immunological process for the determination of the follicle-stimulating hormone (FSH), wherein there is used at least one monoclonal antibody which is directed specifically against FSH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.The present invention also provides a reagent for the determination of the follicle-stimulating hormone, wherein it contains at least one monoclonal antibody which is directed against FSH and cross-reacts with other glycoprotein hormones to an extent of less than 3%.Furthermore, the present invention provides a monoclonal antibody against the follicle-stimulating hormone and a process and a hybridoma cell line for producing it.

Abstract: The present invention provides a process for the determination of peroxidase by the addition of a peroxide and of a chromogen and measurement of the color resulting from the oxidation of the chromogen, the color formation being stopped after a definite time by the addition of a stop agent, wherein catalase is used as stop agent.

Abstract: The present invention provides a process for the preparation of digitalis antibodies in which appropriate mammals are immunized with a digoxin bound to protein, the animal serum is obtained, the immune globulin-containing protein fraction is separated in known manner, the immunologically-active globulins are adsorbed on an immunologically-active column and separated from the other proteins, the antibodies are again eluted from the column and the Fab fractions are split with papain and purified, wherein, as immunologically-active adsorbent, there is used an inorganic matrix of large surface area to which digitoxin aldehyde is bound via a spacer which cannot be split with papain and the splitting off of the Fab fraction from the antibodies is carried out on the matrix.The present invention also provides digitalis antibodies obtained by this process, which antibodies can be used for the therapy of digitalis intoxications and for preparing immunological reagents for the determination of digitalis glycosides.

Abstract: The present invention provides a process for the determination of an immunologically-bindable substance by immunochemical methods in heterogeneous phase, wherein(a) a sample solution containing the substance to be determined is incubated with(b) a solid phase-bound antibody against the bindable substance to be determined(c) a conjugate of the bindable substance to be determined and a Fab fragment of an antibody directed against a determinable enzyme in a definite amount and(d) the enzyme which corresponds to the antibody contained in the conjugate (c),the phases are separated and the enzyme activity is measured in one of the two phases.

Abstract: A process for determining the presence of polyvalent antigens by incubation with three receptors is presented. In addition, a kit for carrying out this process is provided as well. One of said receptors is bound to a solid support and the other two, in solution, derive from the same animal species.

Abstract: The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.

Abstract: The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured.

Abstract: A process for the preparation of immunologically active tagged conjugates by covalent linking of an enzyme with an antigen or haptene, which process comprises placing a solution of a mixture of immunologically active and inactive conjugates in contact with an insoluble complex former able to form a non-immunological complex reversibly with the untagged antigen or haptene component of the conjugate, separating the insoluble complex former, and eluting with a desorbent for untagged antigen or haptene.

Abstract: The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.

Abstract: Process for the preparation of reactive, couplable derivatives of the thyroid hormones 3,3',5-triiodothyronine (T.sub.3) and 3,3',5,5'-tetraiodothyronine (T.sub.4) by reaction with reactive carboxylic acid derivatives, which process comprises sylylating all the functional groups of the thyroid hormone by reaction with a reactive triorganosylyl derivative and then reacting the persylylated derivative of the thyroid hormone thus obtained with an activated carboxylic acid derivative.

Abstract: A method for the determination of creatine kinase-MB in serum by immunological exclusion of sub-unit M and measurement of the sub-unit B for the determination of creatine kinase, the improvement comprising, for the purpose of excluding the sub-unit M, adding to the reaction mixture comprising determination reagents and the creatine kinase-containing sample, monovalent fragments obtained from antibodies against the sub-unit M by proteolytic cleavage under reducing conditions.