Abstract: A self-contained micro-scale gas chromatography system that includes a plurality of gas chromatography components arranged on a micro-fluidic platform with nearly zero dead volume “tubeless” fluidic connections for the gas chromatography components. The micro-fluidic platform includes a plurality of flow channels that provide fluid flow paths for a sample, carrier gas and waste gas through and among the micro-fluidic platform and the plurality of gas chromatography components. The gas chromatography components may include a micro-scale gas chromatography column that is implemented as a MEMS device and includes embedded heating and cooling elements. The system may also include an on-board supply of carrier gas and on-board waste management, as well as a thermal management scheme making the system suitable for use in oil and gas wells and also other remote environments.

Abstract: A self-contained micro-scale gas chromatography system that includes a plurality of gas chromatography components arranged on a micro-fluidic platform with nearly zero dead volume “tubeless” fluidic connections for the gas chromatography components. The micro-fluidic platform includes a plurality of flow channels that provide fluid flow paths for a sample, carrier gas and waste gas through and among the micro-fluidic platform and the plurality of gas chromatography components. The system may also include an on-board supply of carrier gas and on-board waste management, as well as a thermal management scheme making the system suitable for use in oil and gas wells and also other remote environments.

Abstract: The present invention provides methods and apparatus for separating and/or analyzing fluids of interest. According to principles of the present invention, fluid analysis is accomplished with microfluidic devices and may be reported in real-time or near real-time in a subterranean environment. In addition or alternative to oilfield applications, the principles of the present invention contemplate separation in a laboratory or other environment for biological sample separation and analytical chemistry applications. The present invention is capable of separating liquid-liquid mixtures or emulsions in a microfluidic device without fouling.

Abstract: The present invention generally recites a method, system and apparatus for fluid analysis and more specifically recites a method system and apparatus for multistage injection of a fluid to be analyzed into a fluid analysis instrument.

Abstract: Methods and related systems are described for high pressure chromatographic analysis. The described system includes a flowpath adapted to flow a mobile phase and the sample at high pressures, an injector adapted to inject the fluid sample into a flowpath, a separation column adapted to operate at high pressures for separating various components, a detector and a processor that calculates the amount of at least one component of the fluid sample. The system can operate a pressures above 20 atm or even 100 atm, and temperatures above about 100 degrees Celsius. The system can deployed in a wellbore in a subterranean rock formation, and include fluid collection system for obtaining the fluid sample downhole. The system can also be located close to a wellhead and includes a tap in fluid communication with a surface flowline carrying produced fluids and the injector.

Abstract: Methods and related systems are described for improving component separations in chromatography through novel techniques. The improvements in separation is due primarily to the provision of differential acceleration of the components being separated. Various systems and methods for providing differential acceleration are described including: increasing the cross section of the column towards the column outlet, changing the thickness or other composition of stationary phase within the column, and providing a temperature and/or mobile phase velocity gradient along the column.

Abstract: Methods and kits for efficient amplification of nucleic acids are provided. The methods comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having terminal mismatch primer-dimer structure. The methods also comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having nucleotide analogues. The methods enhance efficiency of nucleic acid amplification reaction by reducing non-specific amplification reactions.

Abstract: A heat switch for remote self-contained gas chromatography is disclosed. The device mechanically separates a hot or cold reservoir from the chromatography column when heating or cooling is not needed. The column needs a cooling system to obtain initial temperatures below ambient. At other times the column needs to be heated to relatively high temperatures, during which time the cooling system is preferably detached. The heat switch allows for rapid temperature changes while minimizing the peak cooling power requirement.

Abstract: A self-contained micro-scale gas chromatography system that includes a plurality of gas chromatography components arranged on a micro-fluidic platform with nearly zero dead volume “tubeless” fluidic connections for the gas chromatography components. The micro-fluidic platform includes a plurality of flow channels that provide fluid flow paths for a sample, carrier gas and waste gas through and among the micro-fluidic platform and the plurality of gas chromatography components. The system may also include an on-board supply of carrier gas and on-board waste management, as well as a thermal management scheme making the system suitable for use in oil and gas wells and also other remote environments.

Abstract: A self-contained micro-scale gas chromatography system that includes a plurality of gas chromatography components arranged on a micro-fluidic platform with nearly zero dead volume “tubeless” fluidic connections for the gas chromatography components. The micro-fluidic platform includes a plurality of flow channels that provide fluid flow paths for a sample, carrier gas and waste gas through and among the micro-fluidic platform and the plurality of gas chromatography components. The gas chromatography components may include a micro-scale gas chromatography column that is implemented as a MEMS device and includes embedded heating and cooling elements. The system may also include an on-board supply of carrier gas and on-board waste management, as well as a thermal management scheme making the system suitable for use in oil and gas wells and also other remote environments.

Abstract: A method and apparatus for electrically interfacing between two or more distinct environments. The method and apparatus are directed to an electrical feedthru with one or more electrical transmission lines coated with one or more thin dielectric layers of material to insulate the transmission lines from a feedthru body. The dielectric layers may include a diamond-like carbon coating, which electrically insulates, but also thermally conducts. The use of thin dielectric layers facilitates smaller, more efficient electrical feedthrus. Therefore, the electrical feedthru may be used, for example, with MEMS devices. The electrical feedthru is not limited to any particular geometry, it may be adapted to fit between any two environments.

Abstract: A method for determining formation fluid pressure in earth formation surrounding a borehole wall uses a downhole probe coupled to a variable-volume cavity. The probe is driven into contact with formation at the borehole wall. The method includes expanding the volume of the cavity during a first period of time to establish fluid communication between tool fluid and formation fluid, by withdrawing a minimal amount of fluid from the formation. During a second period of time the tool pressure is allowed to equilibrate to formation pressure. When pressure equilibrium is established, formation fluid pressure is set equal to tool pressure. A preferred embodiment includes terminating expanding the volume of the cavity on detecting a break in the mud cake seal.

Abstract: A method of making a fiber optic accelerometer includes (a) drawing an optical fiber through a resin; (b) winding the resin coated fiber onto a disc mounted on an assembly having a central shaft; and (c) curing the resin-coated fiber. The optical fiber may be drawn through a resin by providing a container filled with a resin having an orifice therethrough and drawing the fiber through the orifice. The resin may be cured such that the fiber is bonded to the disc by curing the resin to the fiber and the disc at the same time.

Abstract: A method and apparatus for electrically interfacing between two or more distinct environments. The method and apparatus are directed to an electrical feedthru with one or more electrical transmission lines coated with one or more thin dielectric layers of material to insulate the transmission lines from a feedthru body. The dielectric layers may include a diamond-like carbon coating, which electrically insulates, but also thermally conducts. The use of thin dielectric layers facilitates smaller, more efficient electrical feedthrus. Therefore, the electrical feedthru may be used, for example, with MEMS devices. The electrical feedthru is not limited to any particular geometry, it may be adapted to fit between any two environments.

Abstract: A method and apparatus for electrically interfacing between two or more distinct environments. The method and apparatus are directed to an electrical feedthru with one or more electrical transmission lines coated with one or more thin dielectric layers of material to insulate the transmission lines from a feedthru body. The dielectric layers may include a diamond-like carbon coating, which electrically insulates, but also thermally conducts. The use of thin dielectric layers facilitates smaller, more efficient electrical feedthrus. Therefore, the electrical feedthru may be used, for example, with MEMS devices. The electrical feedthru is not limited to any particular geometry, it may be adapted to fit between any two environments.

Abstract: A microfluidic system for performing fluid analysis is described having: (a) a submersible housing having a fluid analysis means and a power supply to provide power to said system; and (b) a substrate for receiving a fluid sample, having embedded therein a fluid sample inlet, a reagent inlet, a fluid sample outlet, and a mixing region in fluid communication with the fluid sample inlet, the reagent inlet, and the fluid sample outlet, and wherein the substrate includes a fluid drive means for moving the fluid sample through the substrate, and wherein the substrate interconnects with the housing. At least a portion of the fluid analysis means may be embedded in the substrate.

Abstract: A method and apparatus for electrically interfacing between two or more distinct environments. The method and apparatus are directed to an electrical feedthru with one or more electrical transmission lines coated with one or more thin dielectric layers of material to insulate the transmission lines from a feedthru body. The dielectric layers may include a diamond-like carbon coating, which electrically insulates, but also thermally conducts. The use of thin dielectric layers facilitates smaller, more efficient electrical feedthrus. Therefore, the electrical feedthru may be used, for example, with MEMS devices. The electrical feedthru is not limited to any particular geometry, it may be adapted to fit between any two environments.

Abstract: A multiple mode pre-loadable fiber optic pressure and temperature sensor includes a generally cylindrical structure having at least one compression element, a fiber optic having a Bragg grating in contact with one side of the compression element, a diaphragm in contact with the other side of the compression element, and a fluid port in fluid communication with the diaphragm. According to preferred aspects of the, a groove is provided in at least one compression element for receiving the fiber optic. The sensor is pre-loaded by straining the diaphragm over the adjacent compression element when the cover is attached. The compression element in contact with the diaphragm preferably has a contoured surface contacting the diaphragm and the diaphragm is stretched to match that contour. By varying the contour of the compression element and the thickness of the diaphragm, the dynamic range of the sensor can be changed. The preferred diaphragm has a variable thickness and is made as an integral part of the structure.

Abstract: A fuel cell stack manifold system in which a flexible manifold body includes a pan having a central area, sidewall extending outward from the periphery of the central area, and at least one compound fold comprising a central area fold connecting adjacent portions of the central area and extending between opposite sides of the central area, and a sidewall fold connecting adjacent portions of the sidewall. The manifold system further includes a rail assembly for attachment to the manifold body and adapted to receive pins by which dielectric insulators are joined to the manifold assembly.

Abstract: A method for determining formation fluid pressure in earth formation surrounding a borehole wall uses a downhole probe coupled to a variable-volume cavity. The probe is driven into contact with formation at the borehole wall. The method includes expanding the volume of the cavity during a first period of time to establish fluid communication between tool fluid and formation fluid, by withdrawing a minimal amount of fluid from the formation. During a second period of time the tool pressure is allowed to equilibrate to formation pressure. When pressure equilibrium is established, formation fluid pressure is set equal to tool pressure. A preferred embodiment includes terminating expanding the volume of the cavity on detecting a break in the mud cake seal.