Patents by Inventor Jan Bergstrom
Jan Bergstrom has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20110045574Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: ApplicationFiled: April 21, 2009Publication date: February 24, 2011Inventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Publication number: 20100320149Abstract: The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.Type: ApplicationFiled: July 22, 2008Publication date: December 23, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Andreas Axen, Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Nils Norrman
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Publication number: 20100130354Abstract: The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.Type: ApplicationFiled: January 25, 2010Publication date: May 27, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: JAN BERGSTROM, GUNNAR GLAD, BO-LENNART JOHANSSON, ANDERS LJUNGLOF, JEAN-LUC MALOISEL
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Publication number: 20090281288Abstract: The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.Type: ApplicationFiled: July 21, 2009Publication date: November 12, 2009Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: JAMES VAN ALSTINE, Jan Bergstrom, Henrik Ihre, Tobias Soderman
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Publication number: 20070161000Abstract: The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.Type: ApplicationFiled: February 21, 2005Publication date: July 12, 2007Inventors: James Van Alstine, Jan Bergstrom, Dag Lindstrom, Joachim Stadler
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Publication number: 20070125711Abstract: The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.Type: ApplicationFiled: November 1, 2004Publication date: June 7, 2007Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Anders Ljunglof, Jean-Luc Maloisel
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Publication number: 20060249457Abstract: The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.Type: ApplicationFiled: September 6, 2004Publication date: November 9, 2006Inventors: James Van Alstine, Jan Bergström, Henrik Ihre, Tobias Söderman
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Publication number: 20050267295Abstract: A method for purifying a desired substance by separating from each other a substance (I) from a substance (II), one of which is the desired substance, both of which have affinity for the same ligand structure, and wherein substance (I) is smaller than substance (II). The method comprising the steps of: (i) providing substances I and II in a liquid; (ii) contacting the liquid with an adsorbent which selectively adsorbs substance I; (iii) recovering the desired substance; The adsorbent has (a) an interior part which carries a ligand structure that is capable of binding to substances I and II, and is accessible to substance I, and (b) an outer surface layer that does not adsorb substance II, and is more easily penetrated by substance I than by substance II.Type: ApplicationFiled: March 9, 2005Publication date: December 1, 2005Inventors: Makonnen Belew, Jan Bergstrom, Rolf Berglund, Lennart Soderberg
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Patent number: 6572766Abstract: A matrix is disclosed including a core showing a system of micropores and a surface in which the micropore system has openings. The characterizing feature is that the surface is coated with a polymer (I) having such a large molecular weight that it cannot penetrate into the micropores.Type: GrantFiled: January 24, 2000Date of Patent: June 3, 2003Assignee: Amersham Biosciences ABInventors: Jan Bergström, Rolf Berglund, Lennart Söderberg
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Patent number: 6478042Abstract: A sliding valve having a flow line on which there may be placed a module having flow channel that can be connected and disconnected. The valve avoids the problem of liquid remaining in the flow channel of a first valve component by providing flow channel intersecting segment which intersects both components of the sliding valve so that in a module disconnecting orientation, each valve component defines separate segments of the flow channel while in the connected orientation, the valve components define a single flow channel. The construction allows for liquid flow through the valve independent of the module being in connected or disconnected position.Type: GrantFiled: December 4, 2000Date of Patent: November 12, 2002Assignee: Amersham Pharmacia Biotech ABInventor: Jan Bergström
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Patent number: 6426315Abstract: Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.Type: GrantFiled: March 14, 2000Date of Patent: July 30, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Jan Bergstrom, Rolf Berglund, Lennart Soderberg
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Patent number: 6090288Abstract: Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, .beta.-lactoglobulin 1 and .beta.-lactoglobulin 2 on the anion exchanger is higher than the elution ionic strength required for a quaternary comparative ion exchanger.Type: GrantFiled: December 29, 1998Date of Patent: July 18, 2000Assignee: Amersham Pharmacia Biotech ABInventors: Rolf Berglund, Jan Bergstrom, Makonnen Belew, Lennart Soderberg
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Patent number: 5766460Abstract: A liquid chromatographic system comprises at least two modules, at least one fluid flow line, and at least two connector devices provided along the flow line and respectively connecting the modules to the flow line. One of the two modules comprises a separation module and the other of the two modules comprises an ancillary module having a non-separation function. Each of the two modules includes at least one end connector fit into the respective connector device, and each end connector is adapted to interchangeably fit into either connector device.Type: GrantFiled: January 6, 1997Date of Patent: June 16, 1998Assignee: Pharmacia Biotech ABInventors: Jan Bergstrom, Lennart Soderberg
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Patent number: 5436161Abstract: A matrix coating suitable for use in a biosensor is provided. This matrix coating comprises a hydrogel bound to a surface and via which a desired ligand can be bound. This hydrogel is activated to contain charged groups for bringing about the concentration of biomolecules carrying an opposite charge to that of said charged groups, and reactive groups for covalently binding the biomolecules concentrated to the matrix coating.Type: GrantFiled: July 22, 1994Date of Patent: July 25, 1995Assignee: Pharmacia Biosensor ABInventors: Jan Bergstrom, Stefan Lofaas, Bo Johnsson
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Patent number: 5300861Abstract: The invention provides in an accelerator for accelerating a magnetized rotating plasma comprising a magnetic system arrange symmetrically around an axis, two electrodes (10, 11) extending symmetrically along said axis inside the magnetic system, said electrodes being spaced from each other in the transverse direction of said axis, two pulsed power sources connected to the magnetic system and the electrodes, respectively, and openings (18) in the inner electrode in a cross-section perpendicular to said axis for the supply of a neutral gas to the space defined by said electrodes, a method for controlling the operation of the accelerator wherein the magnetic field is confined to form a layer which comprises a first cylindrical portion (12) with a minor diameter and a second cylindrical portion (14) with a major diameter and a transition portion (13) interconnecting said first and second cylindrical portions being arranged axis-symmetrically around a common axis.Type: GrantFiled: March 16, 1993Date of Patent: April 5, 1994Inventors: Herman Helgesen, Sonja R. Helgesen, Alfred Sillesen, Jan Bergstrom, Vladimir M. Kouznetsov
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Patent number: 5242828Abstract: A sensing surface suitable for use in a biosensor, comprising:a film of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; anda densely packed monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting ofasymmetrical or symmetrical disulfide (--SSR'Y', -SSRY, sulfide (-SR'Y', -SRY), diselenide (-SeSeR'Y', -SeSeRY), selenide (SeR'Y', -SeRY),thiol (-SH), isonitrile, nitro (-NO.sub.2), selenol (-SeH), trivalent phosphorus compounds, isothiocyanate, xanthate, thiocarbamate, phosphine,thio acid and dithio acid (-COSH, -CSSH)where R and R' are hydrocarbon chains optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R' or R may be H, and Y and Y' are active groups for covalently binding ligands or a biocompatible porous matrix.Type: GrantFiled: May 10, 1991Date of Patent: September 7, 1993Assignee: Pharmacia Biosensor ABInventors: Jan Bergstrom, Stefan Lofas, Bo Johnsson
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Patent number: 4249076Abstract: In an optical measuring device for measuring physical magnitudes a sensor is actuated by the physical magnitude to be measured. An optical signal generator and processor provides light signals to the sensor and processes optical output signals transmitted from the sensor. Optical fibers conduct light to the sensor and transmit light signals therefrom to optical detectors. The sensor modulates at least a portion of the light transmitted thereto such that the modulated light output therefrom transmitted to the optic processor contains measuring components representative of the magnitude to be measured and stabilizing component signals independent of the magnitude. The optical processor separates the measuring components and the stabilizing components and generates at least one measuring signal and compensating signals for stabilizing the measuring device.Type: GrantFiled: November 17, 1978Date of Patent: February 3, 1981Assignee: ASEA AktiebolagInventors: Jan Bergstrom, Hans Bertilson, Torgny Brogardh, Anders Persson