Abstract: The present invention relates to a method for the preparation of a purified amylase recovered from a fermentation broth, by the addition of a precipitation agent which is a halide salt, most preferably sodium or potassium chloride. According to a preferred embodiment, a further precipitation agent is added which is an organic compound, most preferably an alkyl ester of 4-hydroxybenzoic acid.The present invention also relates to the purified amylases so obtained and to the uses of these purified amylases.

Abstract: A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.

Abstract: Antifoaming agents are commonly used during culturing of enzyme-producing microorganisms. These antifoams often persist through enzyme processing, slowing filtrations, clogging filtration membranes and adversely affecting the quality of the final product. The present invention describes a method for removing antifoams, and often carbohydrates and pigments, from enzyme systems using mineral clay, the preferred clay being bentonite.

Abstract: This invention contemplates a novel process for the preparation of solution stable alpha-amylase obtained from Bacillus licheniformis, and to the high potency liquid enzyme product prepared by this process. Typically, the enzyme-containing solution from a fermentation is concentrated and starch is added to the concentrate. Alternatively, a precipitation agent such as salt is added to the solution, and a cake containing the enzyme precipitates. The cake is then contacted with an aqueous solution containing starch to extract the enzyme out of the cake to provide a stable liquid enzyme product.

Abstract: Disclosed is a method for enhancing the thermal stability of microbial alpha-amylase. The method involves adding a stabilizing amount of an amphiphile to the enzyme in its aqueous solution. Also included within the scope of the invention is the stabilized alpha-amylase formulation and its use in the liquefaction of starch.

Abstract: Disclosed is a method for causing the dissociation of microbial mycelium and extracellular lipase bound thereto and increasing the measurable activity of the lipase. The method involves treating an aqueous suspension of the mycelium with the anhydride of a dicarboxylic acid which results in dissociation of the mycelium and lipase thereby facilitating recovery of the lipase.

Abstract: Disclosed is a method for the determination of transglucosidase activity in glucoamylase preparations suspected of having TG activity. The method involves inhibiting the glucoamylase in the preparation with acarbose and then measuring the uninhibited TG activity by conventional means.

Abstract: Glucoamylase and acid fungal protease co-produced by the fermentation of fungal species capable of producing these enzymes are separated by carrying out the fermentation at a pH at which the protease associates itself with the fungal mycelium and removing the mycelium from the fermentation broth. The mycelium is then resuspended in an aqueous medium and the pH adjusted to cause the dissociation of the protease and mycelium with subsequent recovery of the protease from the aqueous medium.

Abstract: The present invention relates to a process which comprises recovering proteinaceous materials from nucleoprotein complexes by derivatizing the proteinaceous material with a cyclic anhydride, thereby disassocating the complex, separating the derivatized proteinaceous material from the nucleic acids and subsequently regenerating the proteinaceous material. The recovery of the proteinaceous material is accomplished by maintaining the separated derivatized proteinaceous material at a suitable acidic pH, which is a function of the reaction time and the temperature, until the derivatized proteinaceous material is disassociated into the proteinaceous material and the cyclic anhydride or most usually the equivalent acid and the proteinaceous material is recovered.

Abstract: The process of the invention comprises disrupting microbial protein containing cells; derivatizing the resultant mixture comprising protein and nucleic acid with an acid anhydride; and then isoelectrically precipitating a nucleic acid diminished protein containing fraction from a nucleic acid enriched supernatant.