Abstract: According to one aspect of the present disclosure, a baseband chip may be configured to receive a data stream associated with a channel The baseband chip may be configured to select a first anchor point from the first constellation points of the first transmission layer. The baseband chip may be configured to select a first subset of constellation points from the first constellation points and the second constellation points. The baseband chip may be configured to perform a first iteration of a recursive tree search. The baseband chip may be configured to determine a first path metric based at least in part on the first iteration of the recursive tree search operation. The baseband chip may be configured to select a second anchor point from the second constellation points of the second transmission layer. The second anchor point may be associated with a second iteration of the recursive tree search.

Abstract: A superconducting magnet may include magnet coils including at least one group of outer coils and at least one group of inner coils, a container including an accommodating space, at least one first chamber that is disposed within the accommodating space and houses the at least one group of the inner coils, and at least one second chamber that is disposed within the accommodating space and houses the at least one group of the outer coils. The at least one first chamber and the at least one second chamber may be configured to be filled with a cooling medium and are in fluid communication with each other. The cooling medium may be configured to cool the magnet coils to a superconducting state.

Abstract: An apparatus and method for downlink data transmission and decoding are disclosed. In the method, a physical layer code block or a physical layer code block group is decoded. A medium access control (MAC) sub-packet corresponding to the decoded physical layer code block or the physical code block group is decoded without waiting for any subsequent physical layer code blocks to be decoded. The decoded MAC sub-packet is passed up a protocol stack.

Abstract: A superconducting magnet may include magnet coils including at least one group of outer coils and at least one group of inner coils, a container including an accommodating space, at least one first chamber that is disposed within the accommodating space and houses the at least one group of the inner coils, and at least one second chamber that is disposed within the accommodating space and houses the at least one group of the outer coils. The at least one first chamber and the at least one second chamber may be configured to be filled with a cooling medium and are in fluid communication with each other. The cooling medium may be configured to cool the magnet coils to a superconducting state.

Abstract: A surface cleaning apparatus includes a base unit configured to be moved over a surface to be cleaned, an upper unit coupled with the base unit, and a handle coupled with the upper unit and moveable between a folded position and an upright position.

Abstract: Disclosed is a Bragg mirror, a resonator and a filter device comprised thereof. The Bragg mirror comprises a stack of plurality of layers arranged in an axial direction, wherein the plurality of layers comprises at least one first layer comprising, in a radial direction, a first material and a second material, wherein the first material is a first metal and the second material is a different material with respect to the first material, and wherein the first material is radially embedded by the second material in the first layer, or vice versa. The resonator comprises a top electrode, a bottom electrode, a piezo electric layer arranged between the top electrode and the bottom electrode, a substrate, and a Bragg mirror arranged between the bottom electrode and the substrate.

Abstract: Provided herein are methods and related devices for preprocessing a biological sample during transit. The method may comprise the steps of: storing a biological sample in a storage container having walls that defines a storage volume; transporting the storage container with the stored biological sample to a sample processing facility; controlling one or more storage container parameters during the transporting step to initiate preprocessing of the biological sample; wherein the controlling step improves a processing parameter at the sample processing facility.

Abstract: The invention discloses a method for amplifying antigen-specific regulatory T cells in vitro, belonging to the technical field of biomedicine. The invention adopts Rapamycin combined with TGF-? cells to induce human T cells into antigen-specific regulatory T cells with immunosuppressive function in vitro by the action of DC cells, which has the advantages as follows: 1) the amount is sufficient; 2) the obtained regulatory T cells can resist differentiation to Th17 cells; 3) the obtained regulatory T cells have stronger inhibitory functions and biological effects compared with the regulatory T cells induced by other methods. The invention overcomes many defects of natural regulatory T cells and has greater therapeutic advantages in the treatment of inflammatory diseases, autoimmune diseases and prevention of organ transplant immunological rejection.

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

Abstract: Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation process. The disclosure provides blocking oligonucleotides which bind to byproduct nucleic acid molecules formed during the ligation of adapters to nucleic acid segments prior to sequencing and inhibit or block amplification of the byproduct nucleic acid molecules in subsequent amplification reactions. Methods for library preparation using blocking oligonucleotides are also provided.

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

Abstract: Provided are vertical flow detection devices and related methods. The devices may comprise a membrane having a first surface and a second surface with a plurality of porous structures extending between the first and second surfaces to form fluid conduits from a first fluid chamber formed by the first surface and a second fluid chamber formed by the second fluid surface. A capture agent is immobilized on and/or in the membrane. A rigid porous membrane support mechanically supports the membrane and to provide a relatively uniform flow across the membrane. Various gaskets or holder elements are positioned around an outer edge of the membrane to prevent fluid leakage around the membrane. A fluid pump is configured to force a fluid sample flow in a direction from the first fluid chamber to the second fluid chamber.

Abstract: A superconducting magnet may include magnet coils including at least one group of outer coils and at least one group of inner coils, a container including an accommodating space, at least one first chamber that is disposed within the accommodating space and houses the at least one group of the inner coils, and at least one second chamber that is disposed within the accommodating space and houses the at least one group of the outer coils. The at least one first chamber and the at least one second chamber may be configured to be filled with a cooling medium and are in fluid communication with each other. The cooling medium may be configured to cool the magnet coils to a superconducting state.

Abstract: Provided is an integrated sample collection device for self-collection and analytical pre-processing of a fluid sample, such as a blood sample from a user. A housing is configured to be held by a user, such as a single hand of a user. A contact-activated penetrating member is disposed at least partially in the housing and configured to penetrate skin. A capillary tube is disposed at least partially in the housing and configured to collect blood released by the penetrating member that penetrates the skin. A vacuum-assisted blood collection container can be fluidically connected to the capillary tube for receiving at least a portion of the collected blood. One or more stabilizing/pre-processing agents are provided for stabilizing/pre-processing collected blood in the capillary tube, the vacuum-assisted blood storage container, or both.

Abstract: The present invention relates to a manufactured graphene/metal or metalloid core-shell composite and manufacturing method thereof. The method comprising: using a modified graphene oxide as a base, then performing concentration and steam drying followed by organic solvent replacement to obtain a modified graphene oxide organic solvent; using a liquid-phase self-assembly method to coat the modified graphene oxide onto a surface of the metal or metalloid to form a graphene/metal or metalloid coated particle solution, then filtering and drying to obtain the graphene metal/metalloid core-shell composite. The method improves upon a conventional organic and inorganic material coating technique, and reduces an impact of a water-based solvent and high temperature on a highly reactive metal and metalloid, thereby expanding the feasibility of the coating technique and addressing a barrier of applicability of graphene and reactive metal or metalloid in the field of energetic materials.

Abstract: A method for detecting a gene fusion includes amplifying a nucleic acid sample in the presence of primer pool to produce a plurality of amplicons. The primer pool includes primers targeting a plurality of exon-exon junctions of a driver gene. The amplicons correspond to the exon-exon junctions. The amplicons are sequenced and aligned to a reference sequence. The number of reads corresponding to each amplicon is normalized to give a normalized read count. A baseline correction is applied to the normalized read counts for the amplicons to form corrected read counts. A binary segmentation score is calculated for each corrected read count. A predicted breakpoint for the gene fusion is determined based on the amplicon index corresponding to the maximum absolute binary segmentation score. Gene fusion events may be detected in a partner agnostic manner, i.e. without prior knowledge of the specific fusion partner genes or specific breakpoint information.

Abstract: A communication device is provided that includes a receiver configured to receive a radio frequency signal. The communication device further includes a processor configured to determine a shift frequency based on a component radio frequency signal. The processor is configured to determine a second signal based on the radio frequency signal. The component radio frequency signal is based on the radio frequency signal and associated with a frequency related to the radio frequency signal represented in the frequency domain. The second signal is determined based on shifting frequencies related to the radio frequency signal represented in the frequency domain by the shift frequency.