Abstract: In various example embodiments, systems and methods for administering machine access to a cloud service are presented. An edge manager device in a cloud computing environment can establish a first client for a first application that is executed externally to the cloud computing environment. The edge manager device can provide a first request via a first network to an authorization service application to obtain client identification and client secret information for use by the first client. The edge manager device can receive the client identification and client secret information from the authorization service application via the first network. The client identification and client secret information can be selected by the authorization service application to permit later data access to the edge manager device by the first client. The edge manager device can provide the client identification and client secret information to the first client via a second network.

Abstract: A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.

Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.

Abstract: In various example embodiments, systems and methods for administering machine access to a cloud service are presented. A first device or machine can access an enrollment service in a cloud environment using user-based credential data. The enrollment service can request registration of the first device with an authorization service. If the authorization service accepts the request, then the authorization service can provide credential data for the first device to use to access one or more cloud-based services. In an example embodiment, a third party application provides devices and an enrollment service with credential data that can be used to facilitate device enrollment with cloud services.

Abstract: In various example embodiments, systems and methods for administering machine access to a cloud service are presented. An edge manager device in a cloud computing environment can establish a first client for a first application that is executed externally to the cloud computing environment. The edge manager device can provide a first request via a first network to an authorization service application to obtain client identification and client secret information for use by the first client. The edge manager device can receive the client identification and client secret information from the authorization service application via the first network. The client identification and client secret information can be selected by the authorization service application to permit later data access to the edge manager device by the first client. The edge manager device can provide the client identification and client secret information to the first client via a second network.

Abstract: An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

Abstract: A wafer bonding layer and a process for using the same for bonding wafers are presented. The wafer bonding process includes providing a first wafer, providing a second type wafer and providing a water bonding layer. The wafer bonding layer is provided separately on a contact surface layer of the first or second wafer as part of a CMOS compatible processing recipe.

Abstract: An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

Abstract: Systems, apparatus, and methods are disclosed for fetal monitoring. An example fetal monitoring device includes a removably adherent connector to connect the device to a wearer and conform the device to a contour of the wearer. The example device includes a sensor including epidermal electronics to detect physical signal(s) from the wearer and/or the wearer's fetus and collect data related to the signal(s). The example device includes a communication interface to wirelessly transmit the collected data to an external processing device. The external processing device is to process the collected data to generate an output indicative of a processing of the collected data according to a guideline or standard. The fetal monitoring device is to adhere to the contour of the wearer so as to not interfere with clothing or movement of the wearer and to form a close bond to receive signals from the wearer and her fetus.

Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.

Abstract: A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

Abstract: An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

Abstract: An improved apparatus and method for the capillary electrophoretic separation of chemical compounds comprises carrying out on-line sample preparation by means of semipermeable membranes connected to the capillary separation column for selective introduction of ampholytes into the capillary separation column. Semipermeable membrane means may also be used on-line for preliminary desalting of the sample prior to its introduction into the separation column.

Abstract: The present invention is applied to an analyzer according to which a sample to be analyzed is moved through a capillary tube in liquid chromatography or capillary-zone electrophoretic method. The capillary tube has a separation zone and a detection zone. The capillary tube in the detection zone has a section in which it can vibrate and both ends of this section are fixed.Analytes of the sample introduced into the analyzer are separated from each other in the separation zone. When each of the separated analytes is led to the area irradiated with beam in the detection zone, the capillary tube in the detection zone vibrates. This vibration phenomenon is brought about by tension fluctuation of the capillary tube caused by intermittent irradiation of the detection zone with an excitation beam and absorption of the excitation beam by the analyte. Concentration of analytes in the sample can be measured by detecting amplitude of the vibration.