Patents by Inventor Joachim Klein
Joachim Klein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11976284Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: GrantFiled: March 18, 2022Date of Patent: May 7, 2024Assignee: LONZA LTDInventors: Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
-
Patent number: 11965825Abstract: The present disclosure relates to an in-line compact measuring device, for example for optical measurements, comprising a housing having a process connection intended to be connected to a process vessel connection complementary to the process connection; at least one sensor assembly arranged in the housing; and a measuring circuit that is connected to the sensor assembly and is arranged in the housing. The in-line compact measuring device has at least one fluid line in thermally conductive contact with at least one housing wall of the housing, which fluid line can be connected to a cooling fluid supply arranged outside the housing.Type: GrantFiled: July 30, 2021Date of Patent: April 23, 2024Assignee: Endress+Hauser Conducta GmbH+Co. KGInventors: Hongshuo Domnick, Christian Cramer, Michael Wiederkehr, Moritz Klein, Joachim Mannhardt
-
Publication number: 20230373552Abstract: An adjustment device for a steering wheel may have a first shaft about which the steering wheel is pivotable. A rotary latch is rotationally fixed to the steering wheel by a rotary latch element. A second shaft is arranged in parallel with the first shaft. A coupling lever is arranged on the second shaft to interact with the rotary latch element. The coupling lever has a stop surface which, for the purpose of engagement, engages in at least one corresponding stop surface of the rotary latch element when the steering wheel, and thus also the rotary latch, is pivoted. At least one of the stop surfaces may have a chamfer via which the coupling lever can be displaced or is displaced out of the engagement with the rotary latch element when a predetermined force is applied to the steering wheel.Type: ApplicationFiled: May 17, 2023Publication date: November 23, 2023Inventors: Heinz-Joachim KLEIN, Markus SCHAPS, Thorsten JANSSEN, Martin FASSBENDER, Thomas KREUZ, Philipp KÜPPER
-
Publication number: 20220259608Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: ApplicationFiled: March 18, 2022Publication date: August 18, 2022Inventors: Diethard MATTANOVICH, Brigitte GASSER, Michael MAURER, Roland PRIELHOFER, Joachim KLEIN, Jana WENGER
-
Patent number: 11401523Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: GrantFiled: March 5, 2019Date of Patent: August 2, 2022Assignee: LONZA LTDInventors: Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
-
Publication number: 20220186531Abstract: A locking device for a movable vehicle element of a vehicle may have at least a rotary catch, a locking element and an associated blocking mechanism. The blocking mechanism may have at least one blocking element and a movable actuating element for locked coupling of the rotary catch and the locking element. The movable actuating element may be designed to move the rotary catch into a closed position going beyond at least one latching position and to stop in this closed position. The rotary catch and the blocking element may be out of latching engagement in the closed position.Type: ApplicationFiled: December 14, 2021Publication date: June 16, 2022Applicant: WITTE Automotive GmbHInventors: Thorsten JANSSEN, Dirk JANSSEN, Heinz-Joachim KLEIN
-
Publication number: 20190185866Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: ApplicationFiled: March 5, 2019Publication date: June 20, 2019Inventors: Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
-
Patent number: 10301634Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: GrantFiled: October 26, 2016Date of Patent: May 28, 2019Assignee: LONZA LTD.Inventors: Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
-
Publication number: 20170107525Abstract: Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologouse to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.Type: ApplicationFiled: March 8, 2016Publication date: April 20, 2017Inventors: Johann Brass, SR., Christoph Kiziak, Joachim Klein, Ralf Ostendorp
-
Publication number: 20170037418Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: ApplicationFiled: October 26, 2016Publication date: February 9, 2017Applicant: LONZA LTD.Inventors: DIETHARD MATTANOVICH, BRIGITTE GASSER, MICHAEL MAURER, ROLAND PRIELHOFER, JOACHIM KLEIN, JANA WENGER
-
Patent number: 9512432Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: GrantFiled: October 5, 2012Date of Patent: December 6, 2016Assignee: Lonza Ltd.Inventors: Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
-
Patent number: 9279128Abstract: Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.Type: GrantFiled: October 14, 2011Date of Patent: March 8, 2016Assignee: LONZA AGInventors: Johann Brass, Christoph Kiziak, Joachim Klein, Ralf Ostendorp
-
Publication number: 20140242636Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.Type: ApplicationFiled: October 5, 2012Publication date: August 28, 2014Applicant: LONZA LTD.Inventors: Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
-
Patent number: 8812492Abstract: A system and method is provided for automatic and dynamic adaptation of cache groups in a database system having one or more processors. The method includes analyzing a database query and determining if a set of predicates in a predicate pattern are suitable for inclusion in one or more cache groups, with the one or more cache groups having one or more cache tables; mapping value-based predicates in the predicate pattern to a filling column in the one or more cache tables; and mapping equi-join predicates in the predicate pattern to a referential cache constraint in the one or more cache tables. New cache groups can be created for predicate patterns occurring more frequently and existing cache groups can be deleted if the frequency of the predicate pattern falls below a predetermined threshold value.Type: GrantFiled: December 20, 2011Date of Patent: August 19, 2014Assignee: Software AGInventor: Joachim Klein
-
Patent number: 8735099Abstract: New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.Type: GrantFiled: April 27, 2011Date of Patent: May 27, 2014Assignee: Morphosys AGInventors: Johann Brass, Joachim Klein, Ralf Ostendorp
-
Publication number: 20140090515Abstract: The invention relates to a method for fracture splitting workpieces and to a workpiece that is produced according to such a method. According to the invention, the feed rate and/or the laser pulse is modulated during the laser machining process dependent on the workpiece geometry and/or the laser power.Type: ApplicationFiled: April 10, 2012Publication date: April 3, 2014Applicant: Mauser-Werke Oberndorf Maschinenbau GmbHInventors: Siegfried Gruhler, Willi Breithaupt, Joachim Klein, Horst Schöllhammmer
-
Publication number: 20130159347Abstract: A system and method is provided for automatic and dynamic adaptation of cache groups in a database system having one or more processors. The method includes analyzing a database query and determining if a set of predicates in a predicate pattern are suitable for inclusion in one or more cache groups, with the one or more cache groups having one or more cache tables; mapping value-based predicates in the predicate pattern to a filling column in the one or more cache tables; and mapping equi-join predicates in the predicate pattern to a referential cache constraint in the one or more cache tables. New cache groups can be created for predicate patterns occurring more frequently and existing cache groups can be deleted if the frequency of the predicate pattern falls below a predetermined threshold value.Type: ApplicationFiled: December 20, 2011Publication date: June 20, 2013Applicant: Software AGInventor: Joachim KLEIN
-
Patent number: 8214994Abstract: A method for separating a bearing channel of a housing by cracking. The bearing covers of the bearing channel are separated from the housing by cracking by means of a cracking mandrel. The steps include clamping the housing at the top thereof so that the bearing channel is downwardly oriented, i.e., towards a supporting or clamping surface of the housing and supporting the bearing cover during separation by cracking by means of a counter bracket.Type: GrantFiled: January 27, 2006Date of Patent: July 10, 2012Assignee: Mauser Werke Oberndorf Maschinenbau GmbHInventors: Siegfried Gruhler, Joachim Klein, Bernhard Wössner
-
Publication number: 20120135463Abstract: Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.Type: ApplicationFiled: October 14, 2011Publication date: May 31, 2012Inventors: Johann Brass, Christoph Kiziak, Joachim Klein, Ralf Ostendorp
-
Publication number: 20120077225Abstract: New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.Type: ApplicationFiled: April 27, 2011Publication date: March 29, 2012Inventors: Johann Brass, Joachim Klein, Ralf Ostendorp