Abstract: A microscope assemblage, in particular a confocal laser scanning microscope, has a light source (1), for illumination of a specimen (6). An objective (5) is provided for guiding the illuminating light beam to the specimen (6). A device, having a tilt element, is provided for generating a relative motion between the illuminating light beam and the specimen (6). The device and the tilt are configured so that the illuminating light beam is rotatable in the objective pupil.

Abstract: A microscope (1), preferably a confocal laser scanning microscope, having at least one light source, a detector, and two objectives (2), one of the objectives (2) being arranged on each of the two sides of the specimen plane (3) and the objectives (2) being directed toward one another and having a common focus; and at least one beam splitter (5) for distributing the illuminating light (6) to the objectives (2), and a beam recombiner (5) for combining the detected light (7) coming from the objectives (2), being provided in the illumination/detection beam path (4), is characterized, for selectable, subsequent implementation of ultrahigh-resolution microscope techniques, in that the objectives (2) and the beam splitter/beam recombiner (5) are grouped into a modular assembly (8); and the assembly (8) has an interface (9) for connection to the illumination/detection beam path (4) of the microscope (1).

Abstract: A method for detection of fluorescent light in scanning microscopy includes dividing an illuminating light beam into a plurality of partial illuminating beams so as to illuminate multiple specimen regions simultaneously. Fluorescing materials in a specimen region are excited via multi-photon excitation. Operating parameters of the light source are adapted for optimum fluorescent photon yield to the properties of the fluorescing material in the specimen region. Fluorescent light of the specimen regions is detected simultaneously.

Abstract: The present invention concerns an apparatus for slewing a light beam, having a base element and a support element carrying a light source or an optical component, wherein connecting elements which allow movement of the support element relative to the base element extend between the base element and the support element. The apparatus according to the present invention eliminates or at least reduces the disadvantages of additionally used optical components. The apparatus is characterized in that the connecting elements are spaced apart differently at their ends facing toward the base element and their ends facing toward the support element.

Abstract: A method and an apparatus for phase correction of position and detection signals in scanning microscopy. The method includes generation of a position signal from the position of a beam deflection device (7) and generation, from the light (17) proceeding from the specimen (15), of a detection signal pertinent to the position signal. The position signal and detection signal are then transferred to a processing unit (23). In the processing unit (23), a correction value is determined. The correction value is transferred to a computer (34) to compensate for time differences between the position signal and detection signal.

Abstract: A scanning microscope, in particular a confocal scanning microscope, with a light source (1), preferably a laser, for generating an illumination light beam (14) for a sample (11) and a scanning device for deflecting the illumination light beam (14) is, with a view to fast and reliable image-data acquisition and a compact structure, configured and refined in such a way that the scanning device has at least one micromirror (16). An optical arrangement with a light source (1), preferably a laser, for generating a light beam and at least one micromirror (16) for deflecting the light beam is furthermore provided, in which an adaptive lens (22) is provided for correcting for mirror defects or deformation of the mirror surface.

Abstract: The inventive system comprises a scanning microscope with at least one monitor, a computer and inputting means. Furthermore, at least one laser and control electronics are provided. All of these elements can be arranged on a table top. The laser and the control electronics are stored in a electromagnetically shielded housing wherein the housing can be completely stored under the table top. The housing comprises an U-shaped control panel which embraces a part of the table top when the housing is stored completely under the table top.

Abstract: An optical arrangement in the beam path of a light source suitable for fluorescence excitation, preferably in the beam path of a confocal laser scanning microscope, with at least one spectrally selective element (4) to inject the excitation light (3) of at least one light source (2) in the microscope and to extract the excitation light (3) scattered and reflected on the object (10) or the excitation wavelength from the light (13) coming from the object (10) through the detection beam path (12) is characterized for variable configurations with the simplest construction in that excitation light (3, 9) of different wavelengths can be extracted by the spectrally selective element (4). Alternatively, an optical arrangement like this is characterized in that the spectrally selective element (4) can be adjusted to the excitation wavelength to be extracted.

Abstract: A scanning microscope is disclosed, through which a sample (14) can be illuminated and detected. An illumination pinhole and a detection pinhole (10, 16) are respectively arranged in the illumination beam path and in the detection beam path (8, 15), an optical component (4), which generates at least to some extent spectrally broadened illumination light, is provided in the illumination beam path (8). A polarization-independent and wavelength-independent beam splitter (11) is arranged in a fixed position in the illumination beam path and the detection beam path (8, 15).

Abstract: A scanning microscope has a light source that generates a light beam for illumination of a specimen, and has a beam deflection device with which the light beam can be guided over the specimen. The beam deflection device contains at least one rotatable unit having at least one reflecting mirror. A compensation element that executes a motion equal and opposite to that of the rotatable unit is arranged in such a way that it at least partially compensates for the moment of inertia of the rotatable unit.

Abstract: A scanning microscope has a microscope stand and a light source that emits an illuminating light beam for illumination of a sample. The illuminating light beam is scanned over a sample with a beam deflection device, arranged in the microscope stand. A Bauernfeind prism is arranged between the sample and the detector for deflecting the detection light out of the microscope beam.

Abstract: The apparatus for objective changing comprises an inventory of at least one objective (5) which defines a longitudinal axis (6). The objective change between an objective storage position (22) and a reference objective position (5a) is made possible by the apparatus, and the reference objective position (5a) lies within an optical beam path that defines an optical axis (3). A retaining element defines the reference objective position (5a), and during the objective change, the objective (5) with its longitudinal axis (6) is movable, in the vicinity of the retaining element (26), substantially coaxially with the optical axis (3). The objective change is accomplished along a guide rail (17).

Abstract: A variable diaphragm has two diaphragm blades movable relative to one another. The diaphragm blades are rotatable about a common rotary shaft. The diaphragm can be arranged in a confocal scanning microscope.

Abstract: The present invention relates to an optical arrangement for deflecting a light beam (1, 14), in particular in two substantially mutually perpendicular directions (2, 3), preferably for applying to confocal scanning microscopes, having two mirrors (8, 10) which can be rotated by means of a rotary drive (4, 5) in each case about mutually perpendicular axes—the x-axis (6) and y-axis (7)—one of the two mirrors (8, 10) being assigned a further mirror (9) in a prescribed angular position in a rotationally fixed fashion such that the mutually assigned mirrors (8, 9)—first and second mirrors—rotate jointly about the y-axis (7), and in so doing rotate the light beam (1, 14) about a pivot (11) which lies on the axis of rotation (6)—the x-axis—of the third mirror (10).

Abstract: A laser scanning microscope, preferably a confocal laser scanning microscope, having a laser light source for illuminating a specimen and a detector for detecting the light returning from the specimen, the specimen or parts thereof. The specimen is marked with markers that can be excited to emit. For the specific detection of preferably biological specimen structures, with high localization accuracy for the specimen structures, the laser light source emits exciting light substantially at one wavelength. Different markers emit light of different wavelengths, when irradiated with exciting light of substantially the same wavelength. The detector is embodied as a multi-band detector for the simultaneous detection of light at several wavelengths. A corresponding method for the detection of preferably biological specimens or specimen structures by laser scanning microscopy is described.

Abstract: An apparatus for simultaneous detection of a plurality of spectral regions of a light beam (1), in particular for detection of the light beam (1) of a laser scanner (2) in the detection beam path of a confocal microscope, is characterized, in order to achieve a simple configuration with small overall size and elimination of the defocusing effect, by an arrangement (3) for spectral spreading of the light beam (1) and an arrangement (4) for splitting the spread beam (5) out of the dispersion plane (6) into spectral regions (7, 8, 9), and for subsequent detection of the split spectral regions (7, 8, 9).

Abstract: A method for examining a specimen (11) by means of a confocal scanning microscope having at least one light source (1), preferably a laser, to generate an illuminating light beam (4) for the specimen (11), and a beam deflection device (9) to guide the illuminating light beam (4) over the specimen (11) comprises, in the interest of reliable definition of details or regions of interest of the specimen (11), the following method steps: Firstly a preview image is acquired. Then at least one region of interest in the preview image is marked. This is followed by allocation of individual illuminating light beam wavelengths and/or illuminating light beam power levels to the region or regions. Illumination of the region or regions of the specimen (11) in accordance with the allocation is then accomplished, at least one manipulation in at least one region (25) being performed by means of the illumination.

Abstract: A scanning microscope has at least one illumination source for emitting a light beam, which is fed via a microscope optic to a specimen and scans the latter. In order to correct the imaging defect of the microscope optic, said defect is determined and a correction value is determined therefrom. This correction value is used for influencing control signals which control the impinging of the light beam on the specimen.

Abstract: The invention discloses a system utilized in a scanning microscope and a method for providing user guidance. The system for providing user guidance comprises an illumination source for producing a light beam and optical means for shaping and guiding the light beam. A scanning device scans the light beam across a sample. At least one detector is used to detect the fluorescent or reflected light from the sample. The position signal of the light beam on the sample is also detected. A control and processing unit with digitizing means processes the intensity data received from a sample and generates an online representation of various image quality parameters to be observed by the user on a display of a computer.

Abstract: An arrangement is proposed for detecting fluorescent light from a plurality of specimen points (2), in particular from microgene spots or microbiospots, the specimen points (2) being arranged on a slide (1). This arrangement comprises at least one light source for simultaneously illuminating the specimen points (2) with excitation light, and detection means, comprising detection elements (3), for simultaneously detecting the fluorescent light from the individual specimen points (2). According to the invention, the spacing d between the specimen points (2) and the respectively assigned detection elements (3) is selected to be as small as possible.