Abstract: Methods and compositions for identifying an unknown phenotype of a tissue that correlates with changes in the methylation state of the tissue comprising, nucleic acid sample from the tissue with a reagent that modifies unmethylated cytosine to produce uracil, amplifying the nucleic acid target gene region using at least one primer that hybridizes to a strand of said nucleic acid target gene region to produce amplified nucleic acids, determining the characteristic methylation state of the nucleic acid target gene region by base specific cleavage and identification of methylation sites and comparing the ratio of methylated cytosine to unmethylated cytosine for each methylation site of the nucleic acid target gene region to the ratio of methylated cytosine to unmethylated cytosine for each methylation site of a tissue nucleic acid sample of the same type having a known phenotype thereby identifying the unknown phenotype.

Abstract: Provided herein are libraries of nucleic acid species each comprising a transcription unit having a promoter region operatively linked to a coding sequence. The coding sequence of each nucleic acid species encodes a RNA cleavage substrate comprising a unique compomer species and a cleavage site. Each compomer species has a molecular mass distinguishable from the molecular mass of other compomer species in the library, and cleavage at a cleavage site releases a polynucleotide comprising the compomer species from the RNA cleavage substrate.

Abstract: The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.

Abstract: Provided herein are processes for rapidly identifying or determining sequence information in a sample nucleic acid by comparing sample nucleic acid sequence information to reference nucleic acid sequence information or information obtained from reference samples. Also provided are automated systems for conducting comparative sequence analyses.

Abstract: The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method uses the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and release labels for detection by mass spectrometry. This process is easily incorporated into a PCR amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.

Abstract: The invention relates to an apparatus and a method for ascertaining and correcting the optimum sampling time for an oversampled input bit stream. This involves feeding the data bit blanked with the current sampling phase into the comparative sequence and using the data bit to ascertain a new, corrected sampling phase. This decision-based approach enables the sampling phase to be continuously corrected.

Abstract: The invention relates to an apparatus and a method for ascertaining and correcting the optimum sampling time for an oversampled input bit stream. This involves feeding the data bit blanked with the current sampling phase into the comparative sequence and using the data bit to ascertain a new, corrected sampling phase. This decision-based approach enables the sampling phase to be continuously corrected.

Abstract: A circuit configuration for preparing analog signals for a boundary scan test process includes a boundary cell input structure. A Schmitt trigger receives an analog signal and has an output connected to the boundary cell input structure.

Abstract: A circuit configuration for monitoring a supply voltage of a processor unit being clocked by an oscillator and requiring a minimum supply voltage, includes an undervoltage detector being connected to the processor unit. The undervoltage detector is in operation and generates a reset signal at a supply voltage being in a range between a lower first limit value and a higher second limit value and being below the minimum supply voltage. A comparator is connected to the processor unit. The comparator is in operation at a supply voltage above the second limit value and the comparator generates an activation signal at a supply voltage above a third limit value being above the minimum supply voltage. The oscillator is in operation at a supply voltage above the second limit value and the processor unit is in operation upon the appearance of the activation signal.

Abstract: A hydraulic engine mount is provided, the dynamic characteristics of which may be varied in response to engine performance parameters. In particular, the invention provides for a hydraulic engine mount comprising a core for attachment to an engine and the base for mounting to a frame, the core and the base being connected to each other by an elastic member, at least two fluid filled and elastically deformable chambers divided by a diaphragm, a throttle for permitting a limited exchange of fluid between the chambers, an absorptive mass selectively movable by the core, and a selectively operable connection between the core and the absorptive mass.

Abstract: A hydraulic engine mount comprising two fluid-filled chambers normally communicating with each other to affect a controlled throttled exchange of fluid between them sufficient to absorb relative movements of high frequency low amplitude between an engine and a chassis. Relative movements of high amplitude and low frequency may be absorbed by rendering the mount soft by opening a bypass to provide for a substantially unimpeded flow of fluid between the chambers. Preferably, the bypass is provided with valve means controlled by sensors reacting to parameters representing engine performance or the like.

Abstract: The invention relates to a noise reduction lining of the kind for use in passenger compartments of automobiles for absorbing engine noise of five or six cylinder combustion engines, and comprises between a cover layer and a base layer an absorption layer of open-pored elastic material into which is embedded a perforsted extinction layer.

Abstract: A mount for securing together two relatively displaceable bodies, such as a motor-vehicle engine and a vehicle chassis, with limited relative freedom of movement has a substantially closed liquid-filled chamber formed by a first resiliently deformable wall and a second wall which may be rigid or resiliently deformable. A partition subdivides this chamber into a pair of compartments and has an elastically deformable and relatively stiff portion and an elastically deformable and relatively yieldable portion. One of these portions is formed with a throughgoing orifice. Stops are provided on each side of the relatively yieldable portion to limit the deflection thereof so that low-amplitude vibrations are not transmitted between the bodies, but high-amplitude vibrations are damped when the yieldable portion abuts the stops.

Abstract: In an arrangement according to this disclosure, a spring element (3, 7) is arranged between a mounting core member (2) and a support (6) and is connected to a damper mass (9, 12). The damper mass has a container (10) which is filled with pressure liquid and/or gas and closed by a diaphragm (13). The spring rate of the diaphragm can be varied as desired, particularly in dependence on the speed of rotation of the machine or engine. For varying the spring rate of the diaphragm, a pressure plate (15) is associated with the diaphragm, and is displaceable relative to the diaphragm by way of an adjusting arrangement (16, 17) dependent on the speed of rotation of the machine or engine, and the relative configurations of the pressure plate and the facing diaphragm are such that this displacement reduces or increases the free area of the diaphragm, and thus varies its spring rate.

Abstract: A vibration-absorbing mount with hydraulic damping, especially for mounting an internal-combustion engine upon a vehicle chassis, comprises a housing subdivided internally by an elastic wall which is connected with the force-transmitting member of the mount and is formed with an orifice enabling fluid to pass between the two chambers. For small pressure differentials, the pressure equalization between the two chambers is effected substantially by transfer of fluid through the orifice while large pressure differentials, representing high frequency oscillations, are damped primarily by deformation of the movable wall.