Abstract: Systems and methods of measuring drug efficacy and side effects using non-invasive or husbandry-only testing are described. Steps include testing a cohort with a proposed husbandry-only protocol against an existing gold-standard treatment, and then validating the use of a created surrogate, non-invasive metric in place of an invasive metric. Then, the validated non-invasive surrogate metric and the husbandry-only protocols are used with an animal treatment cohort to study a new proposed treatment. A control cohort is also used, subject to the same husbandry-only testing and the surrogate metric. A statistical difference in outcomes, using one or more surrogate metrics, between the treatment cohort and the control cohort is the drug efficacy, for a drug used to treat the treatment cohort.

Abstract: The field of this invention is recording motion of an animal, uniquely identifying the animal, and recording an activity of that animal. Motions of animals on a path when the animal is or can be uniquely identified are tracklets. Tracklets begin and end at ambiguation events, where these are defined as locations and times of an animal where it cannot be uniquely identified. A first animal may be uniquely identified by first identifying all other animals in the first animal's environment. Animal identification may be after the end of a tracklet. Embodiments use optical flow analysis from video of an animal's environment. Embodiments record an animal's activity on tracklets and then use that activity to measure animal health or use that activity as data for a study using animals.

Abstract: Aggregates of sets of identification tags are described, such as may be used on animals, such as mice or rats, affixed to ears or attached otherwise to an animal. A set may comprise from two to five patterns. An aggregate may comprise one to five sets. Patterns comprise a perimeter with an interior contrasting field, optional contrasting shapes within the field, and an optional contrasting shape core within a shape. Simple shapes may be squares, circles, or triangles. Shapes are selected for maximum machine readability. A pattern may have from zero to three shapes in its field. Patterns within sets are selected for maximum differentiation between the patterns in the set. Aggregates are selected based on the number of different sets required. Identification tags may be used to uniquely identify animals in a cage. Shown are specific patterns for embodiments of devices, systems and methods.

Abstract: A modular vivarium is assembled from pre-manufactured, transportable, sterile modules of distinct types, called pods. A hallway pod forms a spine, with cage-rack pods, ingress/egress pods and optional HVACPIT pods providing HVAC, power and IT services connected as spur pods to the hallway. Supplies and waste are also within modular pods. Temporary or single-use seals cover doorways during transit. Modules are joined or removed while maintaining sterility on both sides of the flush-to-wall doorway junction. A spur pod is sterilely connected to a hallway pod by aligning respective doors proximal, placing a seal between the pods around the doorways, sterilizing air between the pods, then removing the temporary seals from each doorway. Pods may be constructed from easily transportable ISO intermodal containers.

Abstract: A vehicle-to-vehicle (V2V) communication transponder for use in V2V communication, safety, optimization and anti-collision systems wherein the transponder records and shares observed traffic signal timing and phasing, and uses this information to make recommendations or perform automatic operation to optimize parameters such as safety, gas mileage, travel time, and overall traffic flow. Methods for sharing and making recommendations are described. Embodiments include considerations of traffic and road history in recommendations. No central authority, road-side equipment, (RSU), or pre-determined lane maps are required. Embodiments include a hybrid protocol using both TDMA and CSMA. Some embodiments are free of MAC and IP addresses. Embodiments include equipped vehicles and V2V system using the transponder.

Abstract: A test pad comprising multiple test patches and urine detection patches in an array is described. The test pad is suitable for automated urine analysis of multiple animals in a cage. Urine detection patches may be arranged in a BAYER pattern or as rings. Multiple layers, including a substrate, a wicking layer, a test patch layer, and optional protection layer serve to manage puddles of urine. Various isolation elements are described that keep urine puddles from spreading. Color reference spots on the pad are used for dynamic color determination of test patches. Urine detection patches start a read clock and identify which test patches are valid. Elements to hold and align the test pad are described. Methods of detection and reading are described.

Abstract: A method of reading arbitrary symbols is described. Symbols may degrade over time. Shapes of symbols as they degrade are used to update an initial symbol library, which is created based on actual markings, rather than idealized symbols. Marks associate with shapes, which associate to symbols, which in aggregate associate with an object ID. A read mark is compared to all the shapes in the library to determine a most likely shape. A selection set is used to limit shape selection, based on the comparison, to shapes of valid symbols. Comparison methods use probability distributions. Confidence values are used to validate output, generate warnings, and to control updating of the library. Weighted averaging based on confidence values or age of reads may be used at the level of shapes, comparison distributions, or selections.

Abstract: A transceiver in a vehicle-to-vehicle (V2V) communication system regularly broadcasts basic safety messages, comprising location, heading and speed, of a subject vehicle. The transponder is in communication with similar transponders in other vehicles. In order to manage available bandwidth to assure that safety messages are reliably received, the size of the set of vehicles communicating must be managed. Embodiments describe methods of adjusting the power in a consensus feedback series of power management messages. Each power management message comprises both a field comprising the as-transmitted power and also a field comprising a recommended power for other transponders. Transponders receive many such messages and average the recommended power value to compute their own, new, transmit power level. Power level changes are rate-limited.

Abstract: The field of this invention is classifying animal behaviors. In particular the fields of this invention include using animals in vivariums, such as rodents, particularly mice. Animal behaviors are classified according to behaviors consistent with healthy or unhealthy organs or locations within organs, such as the brain. Injected neoplastic cells may be used to create an unhealthy organ or location within an organ. Classifications also include responses to different therapies. The behavior of the animals is observed using fully automatic, continuous monitoring using per-cage ultrasonic and video sensors, where behavior recording is free of human, manual actions. Observed behavior is consistent with healthy or unhealthy behaviors specific to the injection site. Both positive and negative baseline behaviors are collected, typically using the same system or method.

Abstract: The field of this invention is classifying animal behaviors. In particular the fields of this invention include using animals in vivariums, such as rodents, particularly mice. Ultrasonic vocalizations of mice in a vivarium are compared to a predetermined set of positive phenotypes and to a predetermined set of negative phenotypes. The output of those comparisons are then compared to determine a metric of distance to classify vocalizations as closer to or more distant from elements of the positive or negative sets.

Abstract: The field of this invention is classifying animal behaviors. In particular the fields of this invention include using animals in vivariums, such as rodents, particularly mice. In a vivarium cage, animals create ultrasonic vocalizations. In addition, their phenotype changes over time. The vocalizations, a current phenotype, and the cage environments are inputs to a multi-dimensional classifier using clustering algorithms to find multi-dimensional close relationships. Such close relationships may be identified as particular behavior.

Abstract: The field of this invention is classifying animal behaviors. In particular the fields of this invention include using animals in vivariums, such as rodents, particularly mice. In a vivarium cage, mice generate ultrasonic vocalizations and video behaviors. Their phenotype may also change. Starting with known cognitions or emotions, then comparing the vocalizations, changes to phenotype and video behaviors identification, classification and correlations of cognition or emotion may be generated. These new identifications, classifications and correlations are then used to update the starting set of cognition and emotion of the study animals, in a continuous feedback.

Abstract: The field of this invention is classifying animal behaviors. In particular the fields of this invention include using animals in vivariums, such as rodents, particularly mice. Ultrasonic vocalizations are compared to times-shifted video behaviors of the same mice to identify vocalizations that occur prior to, during, and after particular video behaviors. The comparisons generate correlations weights that may then be used to apply known classifications of the video behaviors to the vocalizations.

Abstract: A method of measuring animal health, such as rodent health, such as a degree of arthritis, is described. Steps include capturing video frames, then using optical flow to compute vector fields for each frame, then taking a maximum vector value over a first time period, then selecting a set of maximum vector values over a second time period, then clipping those values to a maximum, then computing an average of those values. This is an animal health metric. Metrics may be organized into a plot over time and may then be compared against known plots to compute an overall animal health and measure efficacy of treatments or other animal characteristics or behavior, including predictive.

Abstract: A method of reading degraded symbols is described. A symbol has an initial shape, which is marked on an object. Marks, shapes, symbols, and object ID are managed separately. A symbol library with symbols and associated shapes is initially created. Shapes in the library are updated from shapes of marks as the marks degrade over time. A read mark is compared to all the shapes in the library to determine a most likely shape. A selection set is used to limit symbol selection, based on the comparison, to valid symbols. The symbol library and selection set may be customized to each usage of the method. Comparison methods use probability distributions. Confidence values are used to validate output, generate warnings, and to control updating of the library. Weighted averaging may be used at the level of shapes, comparison distributions, or selections. One application is reading tattooed marks on rodent tails in a vivarium.

Abstract: A modular vivarium is constructed entirely from transportable, sterile modules of distinct types, called pods. A hallway pod forms a spine, with cage-rack pods, ingress/egress pods and optional VHACPIT pods providing HVAC, power and IT services connected as spur pods to the hallway. Supplies and waste are also within modular pods. Temporary or single-use seals cover doorways during transit. Modules are joined or removed while maintaining sterility on both sides of the flush-to-wall doorway junction. Supply and return air for all pods is through hallway based ducting. Pods may be constructed from ISO intermodal containers. Embodiments have no permanent buildings. Pods may be moved, leveled and aligned for joining via special supports. Cage-rack pods are configured with two cage racks and a single isle permitting robotic cage placement and retrieval. Embodiments provide vibration, noise and earthquake isolation.

Abstract: An animal cage is described suitable for automated use in a vivarium. The cage comprises a study animal; bedding; pathogens; a wireless, a sterilizable scale comprising a sterile case comprising a flexible, penetrated membrane; and a penetrating fastener penetrating the membrane. The comprises four internal volume areas with respect to pathogen content: (1) volume between the membrane and a weighing platform, free of bedding; (2) a sterile volume inside the scale case below the membrane; (3) a vertical channel fluidly connecting the inside of the cage with the first volume; and (4) the inside of the cage outside of the above three volumes. These four volumes are sterilely isolated from air outside the cage. Electronics in the scale communicate wirelessly, through the cage, to cage-associated electronics outside the cage, using narrow beam communications free of device-ID specific protocol. The scale may be disassembled, sterilized in a fluid, re-assembled, and re-used in a different cage.

Abstract: An animal identification code is described comprising two numbers, one of which is encoded into a human-readable marking and the other of which is encoded into a machine-readable marking, where the two numbers and the two encodings are different. The combination of the two numbers, plus additional information not marked on a first animal, such as time of read, is looked up a first table to determine conditional validity and from there to a second table to determine a valid and unique animal ID associated with the animal, a primary key. The animal may be a rodent in a vivarium and the markings may be tattooed on the animal tail. The second marking may be a vine code with a spine where the spine is aligned with the animal tail. The first number may be unique within a first animal population such as an animal study. The combination of the first and second marking may be reused on a second animal wherein the lifetimes of the first and second animal do not overlap.

Abstract: A method of generating animal identification codes is described. A first and second number are selected, then each number is compared respectively against previously used first and previously used second numbers in a pool. Such comparing uses a computed distance based on how the numbers will be encoded and marked on an animal, such as a Hamming distance of printed symbologies. If the distance computed for either the first or second number is below a distance threshold, that number is discarded and another number is selected. When both numbers pass the distance test, they are added as a pair into the pool. Pairs from the pool may be encoded and marked on an animal. The encoding of the first number may generate a human-readable mark while a different encoding of the second number generates a machine-readable mark.

Abstract: A sterile scale is described suitable for automated use in animal cages. A case is covered by a flexible membrane that provides both sealing against pathogens and mechanical compliance for transfer of weight on an above weighing platform through a single sealed penetration in the membrane to a load cell in the case. The membrane comprises a perimeter that is attached and sealed to the case with a membrane frame, a penetration area, penetrated by a rigid weight-bearing element, and an isolating compliance area. A skirt on the weighing platform surrounds the case, protecting detritus from entering from below onto the membrane, while providing open-air movement. The weighing platform and case are easily separated permitting fluid-based sterilization of both. The weight-bearing element may contain an electrical connection from the case to the platform, through the membrane.