Abstract: A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: The present invention relates to a method for treating vegetable oils and/or animal fats. The method comprises adjusting temperature, treatment with acid, adjusting pH, contacting the aqueous mixture with enzyme, crystallization of high melting glycerides and separation.

Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

Abstract: A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: An optical fiber connector device includes an optical fiber connector and an insert unit. The insert unit hash a housing and a connecting mechanism. The housing surrounds an axis, and has an outer wall, a surrounding wall, and a cover member. The outer wall defines a first insert opening on the axis, and has a main opening spaced apart from the axis. The outer wall and the surrounding wall are molded as one piece. The cover member covers removably the main opening. The connecting mechanism has a first connecting member surrounded by the outer wall and connected to the optical fiber connector, and a second connecting member surrounded by the surrounding wall, coupled to the first connecting member, and adapted to be connected to another optical fiber connector.

Abstract: A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

Abstract: A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: An optical fiber connector is for linking a standard connector head member and a lucent connector tail member, and includes a first connecting member and a second connecting member. The first connecting member is adapted to be detachably connected to the standard connector head member, and has a first hollow body that defines a first passage. The second connecting member is adapted to be detachably coupled to the lucent connector tail member, is connected to the first connecting member, and has a second hollow body that defines a second passage. The second passage is in spatial communication with the first passage and has a diameter that is 1.3 millimeters. The first connecting member and the second connecting member are molded as one piece.

Abstract: The present teachings provide an amylase with maltogenic properties. Nucleic acids encoding the maltogenic amylase and variants thereof, expression vectors, formulations, and host cells are also provided. Additional embodiments of the present teachings provide various methods of use and methods of manufacturing.

Abstract: Provided herein are methods of treating, preventing and/or managing cancers, which comprise administering to a patient 3-(5-amino-2-methyl-4-oxo-4H-quinazolin-3-yl)-piperidine-2,6-dione, or an enantiomer or a mixture of enantiomers thereof, or a pharmaceutically acceptable salt, solvate, hydrate, co-crystal, clathrate, or polymorph thereof.

Abstract: The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide.

Abstract: The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

Abstract: An optical fiber connector for connecting to an optical fiber cable includes a shell, a hollow guiding device, and a socket device. The hollow guiding device is connected to the shell. The socket device includes a hollow socket unit and a ferrule. The socket unit is formed as one piece and is detachably connected to an end of the guiding device opposite to the shell such that the optical fiber cable extends through the guiding device and the shell into the socket unit. The ferrule is disposed in the socket unit and is for connecting to one end of the optical fiber cable.

Abstract: A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Abstract: An optical fiber plug adapted to be connected to an optical fiber socket and a first optical fiber cable includes a plug sleeve unit, a plug unit, a plug guiding unit, and a coupling unit. The coupling unit includes a coupling seat, a coupling ring, and a coupling cover movable between a locked position and a released position. When the coupling cover is at the locked position, the optical fiber plug is locked to the optical fiber socket by the coupling ring. When the coupling cover is moved from the locked position to the released position, the coupling ring is urged resiliently by the coupling cover to release the optical fiber plug, thereby permitting separation of the optical fiber plug from the optical fiber socket.

Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract: An optical fiber connector device includes an optical fiber connector and an insert unit. The insert unit has a housing and a connecting mechanism. The housing surrounds an axis, and has an outer wall, a surrounding wall, and a cover member. The outer wall defines a first insert opening on the axis, and has a main opening spaced apart from the axis. The outer wall and the surrounding wall are molded as one piece. The cover member covers removably the main opening. The connecting mechanism has a first connecting member surrounded by the outer wall and connected to the optical fiber connector, and a second connecting member surrounded by the surrounding wall, coupled to the first connecting member, and adapted to be connected to another optical fiber connector.