Abstract: A control arrangement, method, and computer program for obtaining, from a real-time location system, positions of individual animals and of individual mobile agricultural devices located in a livestock area, and automatically controlling one or more of the mobile agricultural devices based on the obtained positions of the mobile agricultural devices and the individual animals so that the mobile agricultural devices are operated optimally and safely of the animals.

Abstract: A peritoneal dialysis (“PD”) system includes a housing, a PD fluid pump housed by the housing, and a reusable patient line extending from the housing. The reusable patient line includes a distal end configured to be connected to a patient line connector provided by the housing. The PD system also includes at least one reusable PD fluid line extending from the housing, the at least one reusable PD fluid line including a distal end configured to be connected to a PD fluid line connector provided by the housing. The PD system further includes a control unit configured to cause the PD fluid pump to apply a negative pressure to at least one of the reusable patient line or the at least one reusable PD fluid line when connected, respectively, to the patient line connector or the PD fluid line connector.

Abstract: A heat exchanger plate module comprises a pressed heat exchanger plate and a flat plate, and a heat exchanger comprises plural of the modules. The plates comprise first and second longitudinal end portions each having a fluid port, and an intermediate heat exchange portion between the first and second longitudinal end portions. The plates further include a pressed corrugated pattern with alternating tops and bottoms in the thickness direction. The pressed pattern comprises in the first and/or second longitudinal end portions, a first fluid channel pattern leading fluid flow into the fluid port and/or a second fluid channel pattern bypassing the fluid port. In the intermediate heat exchange portion, a third fluid channel pattern fluidly communicates with the first and/or second fluid channel pattern and comprises longitudinally extending wave-shaped pressed lines that form discrete longitudinal extending fluid channels, when the pressed heat exchanger plate is attached to the flat plate.

Abstract: The present application provides methods for detecting a target nucleic acid molecule in a sample comprising contacting said sample with a ligatable probe comprising one or more parts and allowing said probe to hybridise to the target nucleic acid molecule, ligating any probe which has hybridised to the target nucleic acid molecule, amplifying the ligated probe, and detecting the amplification product, thereby to detect the target nucleic acid molecule, wherein said probes comprise at least one ribonucleotide at or near to a ligation site and/or wherein the probe or a probe part comprises an additional sequence 5? to a target-specific binding site which is not hybridised to the target nucleic acid molecule upon hybridisation of the probe to the target nucleic acid molecule and forms a 5? flap containing one or more nucleotides at its 3? end that is cleaved prior to ligation, and methods of synthesising a DNA molecule with Phi29 DNA polymerase using a template nucleic acid molecule comprising at least one ribo

Abstract: The present invention relates to a device (10; 30; 50) for analysing liquid samples comprising amplified nucleic acids, said device comprising: a sample pad (16); a first (18) and a second (19) sample analysis strip configured to analyse different aspects of the nucleic acid sample wherein said segments comprise nucleic acid sequences which are complementary to the predetermined sequences of target nucleic acids whose presence or absence is analysed; and a housing (11) enclosing said sample pad and at least two elongated sample analysis strips wherein the analysis result is detectable from the outside of the housing from the combination of aligned segments of said first and second analysis strips. The invention furthermore relates to a method for determining the presence or absence of a target nucleic acid, providing confirmatory results, in combination with a predetermined sequence in a sample from a subject using the device according to the invention.

Abstract: A peritoneal dialysis system includes a cycler, a disposable set operable with the cycler and including a patient line and a drain line, one of (i) a water purifier for supplying purified water for mixing to form fresh dialysis fluid at the disposable set, (ii) at least one fresh dialysis fluid container provided as part of the disposable set for supplying fresh dialysis fluid, or (iii) a dialysis fluid preparation unit configured to supply fresh dialysis fluid to the disposable set, and at least one flow path insulator provided at the cycler, the water purifier, the dialysis fluid preparation unit, and/or along the drain line. The flow path insulator is configured to separate used dialysis fluid flowing along the drain line into flow segments to limit any current flowing from the patient to a drain.

Abstract: A method of assembling resilient floorboards is disclosed that includes the step of bending an edge of a floorboard during the assembling. The bending reduces the force required for connection of the edge to another edge of a juxtaposed floorboard. The floorboards may be provided with a mechanical locking system for vertical and horizontal locking of two adjacent floorboards.

Abstract: The present application provides methods for detecting a target nucleic acid molecule in a sample comprising contacting said sample with a ligatable probe comprising one or more parts and allowing said probe to hybridise to the target nucleic acid molecule, ligating any probe which has hybridised to the target nucleic acid molecule, amplifying the ligated probe, and detecting the amplification product, thereby to detect the target nucleic acid molecule, wherein said probes comprise at least one ribonucleotide at or near to a ligation site and/or wherein the probe or a probe part comprises an additional sequence 5? to a target-specific binding site which is not hybridised to the target nucleic acid molecule upon hybridisation of the probe to the target nucleic acid molecule and forms a 5? flap containing one or more nucleotides at its 3? end that is cleaved prior to ligation, and methods of synthesising a DNA molecule with Phi29 DNA polymerase using a template nucleic acid molecule comprising at least one ribo

Abstract: A set of essentially identical floorboards each including a front face and a rear face extending in the horizontal plane, a core, and a surface layer, a mechanical locking system is arranged at least at two opposite edges for connecting a floorboard with an adjacent floorboard in a horizontal and a vertical direction, said mechanical locking system being configured for connecting the floorboard with the adjacent floorboard by vertical folding, wherein one of said opposite edges is provided with a horizontally extending protrusion at an upper edge, and wherein said protrusion is configured to overlap a surface groove at an upper edge of the other of said opposite edges, such that two connected and adjacent floorboards have upper overlapping edges.

Abstract: The present disclosure in some aspects relate to a direct RNA (dRNA) detection approach incorporating the use of circularizable probes (e.g., padlock probes) having asymmetric arms, rolling circle amplification of the ligated circularizable probes, and in situ detection (e.g., using hybridization-based in situ sequencing) for multiplexed spatial analysis of nucleic acid sequences (e.g., short sequences such as SNPs and point mutations) in a biological sample. In some aspects, compositions and methods disclosed herein improve detection specificity, reduce false positive signal detection, and/or maintain or improve detection efficiency.

Abstract: The present application provides methods for detecting a target nucleic acid molecule in a sample comprising contacting said sample with a ligatable probe comprising one or more parts and allowing said probe to hybridise to the target nucleic acid molecule, ligating any probe which has hybridised to the target nucleic acid molecule, amplifying the ligated probe, and detecting the amplification product, thereby to detect the target nucleic acid molecule, wherein said probes comprise at least one ribonucleotide at or near to a ligation site and/or wherein the probe or a probe part comprises an additional sequence 5? to a target-specific binding site which is not hybridised to the target nucleic acid molecule upon hybridisation of the probe to the target nucleic acid molecule and forms a 5? flap containing one or more nucleotides at its 3? end that is cleaved prior to ligation, and methods of synthesising a DNA molecule with Phi29 DNA polymerase using a template nucleic acid molecule comprising at least one ribo

Abstract: Systems and methods for authenticating objects associated with online activities are provided. For a target object involved in online activities, an authentication system accesses data associated with the target object and generates a featurized dataframe for the target object by processing the data associated with the target object. The authentication system further applies a machine learning model to the featurized dataframe to determine an authenticity indicator for the target object and assigns the target object to an authentication stream according to the authenticity indicator determined. Based on the results returned from the assigned authentication stream, the online activities involving the target object may be blocked or permitted.

Abstract: A method of assembling resilient floorboards is disclosed that includes the step of bending an edge of a floorboard during the assembling. The bending reduces the force required for connection of the edge to another edge of a juxtaposed floorboard. The floorboards may be provided with a mechanical locking system for vertical and horizontal locking of two adjacent floorboards.

Abstract: A diapharagm pump for a bioprocess system comprises a pump head (1; 101; 201; 301) comprising: a common inlet (3); a common outlet (5); a plurality of pump cavities (7) each including at least one cooperating pair of one-way valves, the at least one pair of one-way valves including an inlet valve (9) and an outlet valve (11), wherein the respective inlet valves (9) are in fluid communication with the common inlet (3) and the respective outlet valves (11) are in fluid communication with the common outlet (5) and wherein a centre of the outlet valve (11) for each pump cavity is positionable above a centre of the inlet valve (9) for the same pump cavity when the diaphragm pump is oriented for use to inhibit trapped gas; and a plurality of moveable diaphragms (13) each respectively provided in a respective of said pump cavities (7) for varying a volume of the pump cavities.

Abstract: The present invention relates to the field of biotechnology and a method for creating a cDNA library. More specifically, the invention refers to a method of forming complementary DNA (cDNA) sequencing libraries from RNA in situ comprising the steps of: (a) fixating cells, immobilized on a solid surface; (b) performing an in situ reverse transcription (RT) inside cells, using RT primers comprising a PCR handle 1, and partially biotinylated dNTPs; (c) releasing single stranded cDNA from the cells using a release mix, wherein the release mix comprises an RNAse, such that the single stranded cDNA is released from intact cells; (d) collecting a supernatant comprising released cDNA into a single larger volume or in separate volumes; and (e) introducing an adapter molecule comprising a PCR handle 2 that will bind 3? of the extended cDNA.

Abstract: Operations to blend electronic inventory are provided. The operations may include obtain a request for multiple seats at a venue and determine a combination of data objects from a data storage that represent seats at the venue that meet the request. The combination of the data objects may include at least one of multiple first data objects and at least one of multiple second data objects. The first data objects may be tagged with a first tag and are associated with first seats in the venue controlled by a first entity and the second data objects may be tagged with a second tag and are associated with second seats in the venue controlled by a second entity, and the combination of the data objects is determined irrespective of whether the data objects include the first tag or the second tag.

Abstract: A method of assembling resilient floorboards is disclosed that includes the step of bending an edge of a floorboard during the assembling. The bending reduces the force required for connection of the edge to another edge of a juxtaposed floorboard. The floorboards may be provided with a mechanical locking system for vertical and horizontal locking of two adjacent floorboards.

Abstract: A set of essentially identical floorboards each including a front face and a rear face extending in the horizontal plane, a core, and a surface layer, a mechanical locking system is arranged at least at two opposite edges for connecting a floorboard with an adjacent floorboard in a horizontal and a vertical direction, said mechanical locking system being configured for connecting the floorboard with the adjacent floorboard by vertical folding, wherein one of said opposite edges is provided with a horizontally extending protrusion at an upper edge, and wherein said protrusion is configured to overlap a surface groove at an upper edge of the other of said opposite edges, such that two connected and adjacent floorboards have upper overlapping edges.

Abstract: A set of panels including a panel with a first main plane and an adjacent panel with a second main plane, wherein the panel and the adjacent panel are provided with an angleable locking device for locking a first edge of the panel to a second edge of the adjacent panel. The first main plane is essentially perpendicular to the second main plane. The angleable locking device includes an edge groove at the first edge and a tongue groove at the second edge. The edge groove includes a flexible tongue arranged in an insertion groove at a first side of the edge groove. The angleable locking device is configured to be unlocked by an angling motion of the panel relative the adjacent panel, such that an angle between the first and the second main plane increases.

Abstract: The present invention provides a method for detection of target RNA in a sample comprising the use of a hairpin probe which unfolds upon binding to its target RNA sequence to reveal a 3? end region which is capable firstly of binding to a circular or circularisable probe and if necessary templating ligation of a circularisable probe to circularize the probe, and secondly of priming a subsequent RCA reaction of the circularized/circular probe. The circularized or circular probe is then subjected to RCA and a RCA product is detected, in order to detect the target RNA. Also provided are probes and kits for performing such a method.