Abstract: A method of preventing or inhibiting L-cystine crystallization is disclosed, using the compounds of formula I: R1a—[O]v-(-A-L-)m-A-[O]v—R1b??I wherein A, L, R1a, R1b, m, and v are as described herein. The compounds may be prepared as pharmaceutical compositions, and may be used for the prevention and treatment of conditions that are causally related to L-cystine crystallization, such as comprising (but not limited to) kidney stones.

Abstract: An apparatus for separating particles from a medium includes a capillary defining a flow path therein that is in fluid communication with a medium source. The medium source includes engineered acoustic contrast capture particle having a predetermined acoustic contrast. The apparatus includes a vibration generator that is operable to produce at least one acoustic field within the flow path. The acoustic field produces a force potential minima for positive acoustic contrast particles and a force potential minima for negative acoustic contrast particles in the flow path and drives the engineered acoustic contrast capture particles to either the force potential minima for positive acoustic contrast particles or the force potential minima for negative acoustic contrast particles.

Abstract: A method of preventing or inhibiting L-cystine crystallization is disclosed, using the compounds of formula I: R1a—[O]v-(-A-L-)m-A-[O]v—R1b??I, wherein A, L, R1a, R1b, m, and v are as described herein. The compounds may be prepared as pharmaceutical compositions, and may be used for the prevention and treatment of conditions that are causally related to L-cystine crystallization, such as comprising (but not limited to) kidney stones.

Abstract: A method of forming a hybridoma cell includes acoustically focusing antibody producing cells in a first channel having a first acoustic field so as to produce a single file line of antibody producing cells; acoustically focusing immortal cells in a second channel having a second acoustic field so as to produce a single file line of immortal cells; flowing the acoustically focused single file lines of antibody producing cells and immortal cells to a third channel having a third acoustic field configured to bring at least one antibody cell and at least one immortal cell into close enough proximity to permit them to fuse; and fusing the antibody producing cell and the immortal cell together by at least one of a chemical and an electrical means to form at least one hybridoma cell.

Abstract: A method of focusing particles is provided. The method includes transiting a fluid containing particles therein through a channel at a flow rate and adjusting the flow rate for a desired transit time through an interrogation zone through which a light from an excitation source passes. The method further includes optically exciting the particles with the excitation source, detecting an optical signal from the particles, and analyzing the optical signal. The particles may be droplets. Further, the particles may transit the interrogation zone in single file. A system of focusing particles is also provided. The system includes a channel having an inlet for accepting a fluid containing particles. The system further includes a flow adjuster configured to adjust the flow rate for a desired transit time through an interrogation zone, a light source configured to optically excite the particles, and a detector configured to detect optical signals from the particles.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: An apparatus for separating particles from a medium includes a capillary defining a flow path therein that is in fluid communication with a medium source. The medium source includes engineered acoustic contrast capture particle having a predetermined acoustic contrast. The apparatus includes a vibration generator that is operable to produce at least one acoustic field within the flow path. The acoustic field produces a force potential minima for positive acoustic contrast particles and a force potential minima for negative acoustic contrast particles in the flow path and drives the engineered acoustic contrast capture particles to either the force potential minima for positive acoustic contrast particles or the force potential minima for negative acoustic contrast particles.

Abstract: A method for optimizing particle throughput in a particle analyzer includes determining an optimal concentration of particles in a sample for achieving a user defined coincidence rate; adjusting the concentration of the particles in the sample to the determined optimal concentration to achieve the user defined coincidence rate; acoustically focusing the particles in the particle analyzer; adjusting a flow rate of the particles to achieve a user defined transit time of the particles; and analyzing at least some of the particles with an interrogation source.

Abstract: An acoustic focusing capillary includes a capillary coupled to at least one vibration source possessing a groove. A method of manufacturing such an acoustic focusing capillary includes providing a capillary and a vibration source, machining a groove into the vibration source, and coupling the vibration source to the capillary at the groove. Another method relates to focusing a particle stream and includes flowing a sheath fluid into an outer confine of a capillary, flowing a particle stream into a central core of the capillary, and acoustically focusing the particle stream by applying acoustic radiation pressure to the particle stream at a first location along the capillary. The particle stream may be further focused by hydrodynamically focusing it at a second location along the capillary downstream of the first location.

Abstract: A method of preventing or inhibiting L-cystine crystallization using the compounds of formula I is disclosed. wherein A, L, R1a, R1b, and m are as described herein. The compounds may be prepared as pharmaceutical compositions, and may be used for the prevention and treatment of conditions that are causally related to L-cystine crystallization, such as comprising (but not limited to) kidney stones.

Abstract: A method to produce an assay is provided. The method includes introducing into a channel having a peripheral wall a first fluid having a first acoustic contrast, and introducing into a substantially peripheral portion of the channel a second fluid having a second acoustic contrast that is different from the first acoustic contrast, the substantially peripheral portion reaching from within the channel to the peripheral wall of the channel, wherein the second fluid is a biological fluid. The method further includes applying acoustic radiation pressure to the channel, and acoustically reorienting the second fluid based upon the difference between the first acoustic contrast and the second acoustic contrast such that the acoustically reoriented second fluid is translated from the substantially peripheral portion of the channel to a substantially internal portion of the channel that does not reach the peripheral wall of the channel.

Abstract: A method of preventing or inhibiting L-cystine crystallization using the compounds of formula I is disclosed. R1a—O-(-A-L-)m-A-O—R1b??I, wherein A, L, R1a, R1b, and m are as described herein. The compounds may be prepared as pharmaceutical compositions, and may be used for the prevention and treatment of conditions that are causally related to L-cystine crystallization, such as comprising (but not limited to) kidney stones.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: A method of preventing or inhibiting L-cystine crystallization is disclosed, using the compounds of formula I: R1a—[O]v-(-A-L-)m-A-[O]v—R1b??I wherein A, L, R1a, R1b, m, and v are as described herein. The compounds may be prepared as pharmaceutical compositions, and may be used for the prevention and treatment of conditions that are causally related to L-cystine crystallization, such as comprising (but not limited to) kidney stones.

Abstract: The present invention is a method and apparatus for acoustically manipulating one or more particles.

Abstract: The present invention is a method and apparatus for acoustically manipulating one or more particles.

Abstract: A method for fractionating particles includes flowing a fluid having particles therein along a first channel, applying acoustic radiation pressure to the fluid, focusing the particles within the fluid into a single file line, moving the particles in a flow rate, applying acoustic radiation pressure to the fluid for a second time, focusing the particles based on size and acoustic contrast, producing at least two fluid fractions of the particles, and collecting at least one of the fractions.

Abstract: The present invention is a method and apparatus for acoustically manipulating one or more particles. Acoustically manipulated particles may be separated by size. The particles may be flowed in a flow stream and acoustic radiation pressure, which may be radial, may be applied to the flow stream. This application of acoustic radiation pressure may separate the particles. In one embodiment, the particles may be separated by size, and as a further example, the larger particles may be transported to a central axis.

Abstract: The present invention is a method and apparatus for acoustically manipulating one or more particles. Acoustically manipulated particles may be separated by size. The particles maybe flowed in a flow stream and acoustic radiation pressure, which may be radial, may be applied to the flow stream. This application of acoustic radiation pressure may separate the particles. In one embodiment, the particles may be separated by size, and as a further example, the larger particles may be transported to a central axis.