Abstract: The present invention relates to isolated or non-natural nucleic acids encoding at least a portion of an avian Mago protein or a variant thereof. Nucleic acids comprising all or part of the Mago nashi-encoding region and/or an untranslated region of a Mago nashi cDNA are used as probes for hybridizing and detecting nucleic acids encoding all or part of a Mago protein. Recombinant cells, tissues and animals containing recombinant nucleic acids including expression vectors and encoding Mago, antibodies to the Mago proteins, assays utilizing the Mago polypeptide are within the scope of the present invention. Recombinant nucleic acid molecules may contain transcription regulatory sequences, a sequence complementary to a mRNA sequence encoding a Mago-related polypeptide and transcriptional control sequences functional in a recipient cell.

Abstract: The present invention includes methods of producing an integrated transgene in an avian cell which include introducing a nucleic acid into an avian cell by electroporating and methods of producing a transgenic avian comprising injecting the cell comprising the transgene into an avian stage X embryo. The present invention also provides for methods of screening for nucleic acid integration in a cellular genome which include transforming a nucleic acid comprising a marker into a recipient avian cell and determining if the nucleic is present in equal copy number in cells of a colony produced by the recipient avian cell.

Abstract: This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced.

Abstract: This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced.

Abstract: This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.

Abstract: This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.

Abstract: The present invention relates to isolated or non-natural nucleic acids encoding at least a portion of an avian Mago protein or a variant thereof. Nucleic acids comprising all or part of the mago nashi-encoding region and/or an untranslated region of a mago nashi cDNA are used as probes for hybridizing and detecting nucleic acids encoding all or part of a Mago protein. Recombinant cells, tissues and animals containing recombinant nucleic acids including expression vectors and encoding Mago, antibodies to the Mago proteins, assays utilizing the Mago polypeptide are within the scope of the present invention. Recombinant nucleic acid molecules may contain transcription regulatory sequences, a sequence complementary to a mRNA sequence encoding a Mago-related polypeptide and transcriptional control sequences functional in a recipient cell.

Abstract: A method of manipulating an egg or an embryo therein, preferably a chicken egg or embryo, and sealing the egg shell with a heat melted composition. The present invention provides a method whereby a hole in an egg shell may be optionally resealed with a patch from an egg shell membrane and covered by a heat sealed composition. The present invention further provides a method to expel air introduced into the egg during manipulation by expanding the air sac of the egg.