Abstract: An additive manufacturing machine and an energy beam system including an energy beam generator configured to output an energy beam through a first optical element along a first direction is provided. The energy beam system includes an optical translation system positioned to receive the energy beam through a steering optic. The steering optic is positioned within a translator apparatus. The translator apparatus is configured to translate the steering optic along a plane perpendicular to the first direction.

Abstract: Additive manufacturing methods and systems are disclosed including irradiation devices for an additive manufacturing machine for additively manufacturing three-dimensional objects. The irradiation device includes a beam generation device configured to provide an energy beam travelling on a nominal beam path trajectory and an optical modulator comprising a reflective optic downstream from the beam generating device, wherein the optical modulator is configured to actuate the reflective optic to modify a position of the energy beam from the nominal beam path trajectory. The irradiation device further includes an optical scanner disposed downstream from the optical modulator, wherein the optical scanner is configured to translate the nominal beam path trajectory along a build plane of the additive manufacturing machine.

Abstract: Methods of additively manufacturing a three-dimensional object include irradiating a first build plane region using a first energy beam, irradiating a second build plane region using a second energy beam, and irradiating an interlace region between the first build plane region and the second build plane region. Irradiating the interlace region comprises directing the first energy beam along a first oscillating path and directing the second energy beam along a second oscillating path intersecting and overlapping with the first oscillating path.

Abstract: A system includes a first group of optic lenses within a focusing unit positioned along the propagation direction of a collimated laser beam, the first group of optic lenses separated by a predetermined fixed distance. The first group of optic lenses in conjunction cause the collimated beam to form as an annular beam as it passes through the first group of optic lenses. An axicon lens located distal from the first group of optic lenses along the propagation direction, the axicon lens operable to bifurcate the annular beam into two deflected collimated beam sections, and the axicon lens having a focus that causes the two deflected collimated beam sections to merge at a distance distal from the axicon lens to create an interference pattern region.

Abstract: A system includes a first group of optic lenses within a focusing unit positioned along the propagation direction of a collimated laser beam, the first group of optic lenses separated by a predetermined fixed distance. The first group of optic lenses in conjunction cause the collimated beam to form as an annular beam as it passes through the first group of optic lenses. An axicon lens located distal from the first group of optic lenses along the propagation direction, the axicon lens operable to bifurcate the annular beam into two deflected collimated beam sections, and the axicon lens having a focus that causes the two deflected collimated beam sections to merge at a distance distal from the axicon lens to create an interference pattern region.

Abstract: An additive manufacturing system includes a laser device, a build plate, and a scanning device. The laser device is configured to generate a laser beam with a variable intensity. The build plate is configured to support a powdered build material. The scanning device is configured to selectively direct the laser beam across the powdered build material to generate a melt pool on the build plate. The scanning device is configured to oscillate a spatial position of the laser beam while the laser device simultaneously modulates the intensity of the laser beam to facilitate reducing spatter and to facilitate reducing a temperature of the melt pool to reduce overheating of the melt pool.

Abstract: An additive manufacturing system includes a laser device, a build plate, and a scanning device. The laser device is configured to generate a laser beam with a variable intensity. The build plate is configured to support a powdered build material. The scanning device is configured to selectively direct the laser beam across the powdered build material to generate a melt pool on the build plate. The scanning device is configured to oscillate a spatial position of the laser beam while the laser device is configured to simultaneously modulate the intensity of the laser beam to thermally control the melt pool.

Abstract: A system includes a first group of optic lenses within a focusing unit positioned along the propagation direction of a collimated laser beam, the first group of optic lenses separated by a predetermined fixed distance. The first group of optic lenses in conjunction cause the collimated beam to form as an annular beam as it passes through the first group of optic lenses. An axicon lens located distal from the first group of optic lenses along the propagation direction, the axicon lens operable to bifurcate the annular beam into two deflected collimated beam sections, and the axicon lens having a focus that causes the two deflected collimated beam sections to merge at a distance distal from the axicon lens to create an interference pattern region.

Abstract: The present approach generally relates to systems and methods for implementing energy modulated tomographic imaging of nanoparticles. In certain embodiments, a first energy is used to activate probe particles labeling an anatomy or tissue of interest. The probe particles, once activated, emit photons at a different rate and/or spectrum in response to an underlying physiological event, such as action potentials propagating in the labeled anatomy or tissue. The emitted photons may then be detected and used to map or image the occurrence of the physiological event.

Abstract: An additive manufacturing system includes a laser device, a build plate, and a scanning device. The laser device is configured to generate a laser beam with a variable intensity. The build plate is configured to support a powdered build material. The scanning device is configured to selectively direct the laser beam across the powdered build material to generate a melt pool on the build plate. The scanning device is configured to oscillate a spatial position of the laser beam while the laser device simultaneously modulates the intensity of the laser beam to facilitate reducing spatter and to facilitate reducing a temperature of the melt pool to reduce overheating of the melt pool.

Abstract: An additive manufacturing system includes a laser device, a build plate, and a scanning device. The laser device is configured to generate a laser beam with a variable intensity. The build plate is configured to support a powdered build material. The scanning device is configured to selectively direct the laser beam across the powdered build material to generate a melt pool on the build plate. The scanning device is configured to oscillate a spatial position of the laser beam while the laser device is configured to simultaneously modulate the intensity of the laser beam to thermally control the melt pool.

Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, defecting and bleaching may be iteratively repeated.

Abstract: The present invention provides a microscope slide for analyzing a tissue sample. The slide includes an elongate planar substrate, which comprises a first major surface having a sample-affixing area within which a tissue sample may be affixed; a first positive control sample affixed on the first major surface at a first location adjacent to the sample-affixing area; and a second positive control sample affixed on the first major surface at a second location adjacent to the sample-affixing area. The first and second locations are spaced such that quality of the staining of the first and second positive control samples is indicative of the quality of the staining of the tissue sample. Also provided are a kit containing the microscope slide and a method of making and a method of using the microscope slide.

Abstract: A method for processing and imaging a first and second plurality of samples, comprising processing at least one sample from the first plurality of samples, imaging the at least one sample from the first plurality of samples, while being capable of simultaneously processing at least one sample from the second plurality of samples; and imaging the at least one processed sample from the second plurality of samples.

Abstract: The present approach generally relates to systems and methods for implementing energy modulated tomographic imaging of nanoparticles. In certain embodiments, a first energy is used to activate probe particles labeling an anatomy or tissue of interest. The probe particles, once activated, emit photons at a different rate and/or spectrum in response to an underlying physiological event, such as action potentials propagating in the labeled anatomy or tissue. The emitted photons may then be detected and used to map or image the occurrence of the physiological event.

Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.

Abstract: The present invention provides a microscope slide for analyzing a tissue sample. The slide includes an elongate planar substrate, which comprises a first major surface having a sample-affixing area within which a tissue sample may be affixed; a first positive control sample affixed on the first major surface at a first location adjacent to the sample-affixing area; and a second positive control sample affixed on the first major surface at a second location adjacent to the sample-affixing area. The first and second locations are spaced such that quality of the staining of the first and second positive control samples is indicative of the quality of the staining of the tissue sample. Also provided are a kit containing the microscope slide and a method of making and a method of using the microscope slide.

Abstract: An ultra-compact microscope configured to image at least a portion of a biological sample is provided. The ultra-compact microscope includes an illumination source configured to provide illumination beams to image at least the portion of the biological sample. The ultra-compact microscope further includes an image sensor configured to acquire emitted signals from at least the portion of the biological sample. Moreover, the ultra-compact microscope includes an objective lens operatively coupled to the image sensor and configured to direct emitted signals from the biological sample to the image sensor. Additionally, the ultra-compact microscope includes a micro-motion control assembly operatively coupled to the image sensor and configured to provide dynamic translational motion, dynamic tilting motion, or both to the image sensor at least during scanning of the biological sample.

Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, defecting and bleaching may be iteratively repeated.

Abstract: A method for processing and imaging a first and second plurality of samples, comprising processing at least one sample from the first plurality of samples, imaging the at least one sample from the first plurality of samples, while being capable of simultaneously processing at least one sample from the second plurality of samples; and imaging the at least one processed sample from the second plurality of samples.