Patents by Inventor Sailaja Chandrapati
Sailaja Chandrapati has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230049162Abstract: Composition useful for, among other things, culturing and enumerating microorganisms, and associated methods.Type: ApplicationFiled: January 4, 2021Publication date: February 16, 2023Inventor: Sailaja Chandrapati
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Publication number: 20220315977Abstract: Article for sampling comprising inner layer, one or more outer layers, and optional scouring layer on a portion of the one or more outer layers.Type: ApplicationFiled: August 17, 2020Publication date: October 6, 2022Inventors: Sailaja Chandrapati, Saurabh Batra, Stephen M. Sanocki, Walter E. Partlo, Lou D. Hibbard, Joseph A. Dunbar
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Patent number: 11414641Abstract: A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.Type: GrantFiled: December 10, 2020Date of Patent: August 16, 2022Assignee: 3M INNOVATIVE PROPERTIES COMPANYInventors: Sailaja Chandrapati, Tera M. Nordby
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Patent number: 11319573Abstract: A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a ?-D-glucuronidase indicator system that comprises a plurality of compounds that enhance ?-glucuronidase enzyme activity in E. coli, a redox indicator system and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. Methods of using the device are also provided.Type: GrantFiled: April 2, 2018Date of Patent: May 3, 2022Assignee: 3M INNOVATIVE PROPERTIES COMPANYInventors: Sailaja Chandrapati, Alec J. Teagarden, Haley A. Saddoris
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Publication number: 20210087516Abstract: A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.Type: ApplicationFiled: December 10, 2020Publication date: March 25, 2021Inventors: Sailaja Chandrapati, Tera M. Nordby
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Patent number: 10947577Abstract: A biological sterilization indicator device is provided. The device comprises a body, a plurality of test microorganisms, and an oxygen-modulated first fluorescent sensor. The body comprises a first layer attached to a second layer, forming at least one isolatable microchamber and at least one primary passageway that provides fluidic communication between ambience and the at least one microchamber. The microchamber has an isolated volume of about 0.5 microliters to about 9.5 microliters. The plurality of test microorganisms and the oxygen-modulated first fluorescent sensor are disposed in the microchamber. A method of using the device to determine the effectiveness of a sterilization process is also provided.Type: GrantFiled: February 11, 2013Date of Patent: March 16, 2021Assignee: 3M Innovative Properties CompanyInventors: Francois Ahimou, Sailaja Chandrapati, Kurt J. Halverson, Heather M. Webb, Michael G. Williams
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Publication number: 20200399574Abstract: A device for growing microorganisms is provided. The device includes a body member comprising a self-supporting, waterproof substrate, a layer of adhesive disposed on the substrate, a powder adhered to the adhesive, a cover sheet adhered to the body member, and a dry composition including a mixture of hydroxypropylmethylcellulose and guar gum adhered to the cover sheet or the substrate. The powder comprises a cold-water-soluble gelling agent and one or more nutrient for growing microorganisms. The composition, when reconstituted with water, resists liquefaction by microorganisms A method of culturing microorganisms using the device is also provided.Type: ApplicationFiled: December 12, 2018Publication date: December 24, 2020Inventors: Jason W. Bjork, Sailaja Chandrapati
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Patent number: 10640743Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.Type: GrantFiled: January 25, 2018Date of Patent: May 5, 2020Assignee: 3M Innovative Properties CompanyInventors: Sailaja Chandrapati, Tera M. Nordby
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Publication number: 20200109431Abstract: A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a ?-D-glucuronidase indicator system that comprises a plurality of compounds that enhance ?-glucuronidase enzyme activity in E. coli, a redox indicator system and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. Methods of using the device are also provided.Type: ApplicationFiled: April 2, 2018Publication date: April 9, 2020Inventors: Sailaja CHANDRAPATI, Alec J. TEAGARDEN, Haley A. SADDORIS
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Publication number: 20200048679Abstract: A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a ?-D-glucuronidase indicator system, and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. The first sheet and second sheet are configured to retard passage of carbon dioxide therethrough. Methods of using the device are also provided.Type: ApplicationFiled: April 2, 2018Publication date: February 13, 2020Inventors: Sailaja CHANDRAPATI, Alec J. TEAGARDEN, Haley A. SADDORIS
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Patent number: 10047334Abstract: A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.Type: GrantFiled: March 9, 2016Date of Patent: August 14, 2018Assignee: 3M Innovative Properties CompanyInventors: Sailaja Chandrapati, Heather M. Webb, Jeffrey C. Pederson, Jeffrey D. Smith, RuthAnn R. Duda, Brian J. Engel
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Publication number: 20180148675Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.Type: ApplicationFiled: January 25, 2018Publication date: May 31, 2018Inventors: SAILAJA CHANDRAPATI, TERA M. NORDBY
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Patent number: 9879214Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.Type: GrantFiled: July 30, 2015Date of Patent: January 30, 2018Assignee: 3M Innovative Properties CompanyInventors: Sailaja Chandrapati, Tera M. Nordby
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Patent number: 9717812Abstract: A biological sterilization indicator, system, and methods of determining the effectiveness of a sterilization process. The biological sterilization indicator can include a locus of spores, a reservoir containing a liquid, and a sterilant path positioned to provide fluid communication between ambience and the locus of spores. The reservoir can have a closed state in which the reservoir is not in fluid communication with the locus of spores and an open state in which the reservoir is in fluid communication with the locus of spores. The biological sterilization indicator system can include the biological sterilization indicator and a detection device adapted to be coupled to the biological sterilization indicator. In some embodiments, the method can include assaying the spores for a detectable change in a characteristic, and detecting substantially all of the detectable change.Type: GrantFiled: February 18, 2015Date of Patent: August 1, 2017Assignee: 3M INNOVATIVE PROPERTIES CO.Inventors: Sailaja Chandrapati, Jeffrey C. Pederson, Heather M. Webb, Jeffrey D. Smith, Kurt J. Halverson, Bryan S. Behun, Peter D. Ludowise
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Patent number: 9701996Abstract: A biological sterilization indicator (BI) and a method of using same for assaying the lethality of a sterilization process. The BI can include a housing, which can include a first portion, and a second portion, which can be movable with respect to the first portion between a first and second position. The BI can further include a frangible container comprising a liquid. The BI can further include a spore reservoir and a projection positioned in the housing. The projection can be configured to fracture the container when the second portion of the housing is moved from the first position to the second position. The method can include maintaining a minimal cross-sectional area of space around the container when the second portion of the housing is in the first position, and fracturing the container in response to moving the second portion between the first and second positions.Type: GrantFiled: February 17, 2015Date of Patent: July 11, 2017Assignee: 3M Innovative Properties CompanyInventors: Jeffrey D. Smith, Jeffrey C. Pederson, Sailaja Chandrapati
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Publication number: 20170073629Abstract: A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.Type: ApplicationFiled: March 5, 2015Publication date: March 16, 2017Applicant: 3M Innovative Properties CompanyInventors: SAILAJA CHANDRAPATI, TERA M. NORDBY
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Publication number: 20170037447Abstract: A sterility indicating composition comprising a plurality of sterilization process resistant spores; a germination medium comprising a sub-lethal amount of at least one cell-permeant nucleic acid-interacting fluorescent dye and at least one nutrient for germination of the spores; wherein the at least one cell-permeant fluorescent dye can interact with nucleic acids present in and produced by the plurality of spores during germination or during germination and outgrowth of the spores to produce an increase in fluorescence intensity, indicating that viable spores are present, and wherein the cell-permeant fluorescent dye is sufficiently stable at least at a temperature for incubating the spores to produce the increase in fluorescence intensity, a sterilization process indicator comprising the composition, and a method of determining the effectiveness of a sterilization process using the composition and indicator are disclosed.Type: ApplicationFiled: October 24, 2016Publication date: February 9, 2017Inventors: SAILAJA CHANDRAPATI, HEATHER M. WEBB, KURT J. HALVERSON
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Patent number: 9540677Abstract: A biological sterilization indicator (BI) and method of using same. The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a first fluid path positioned to fluidly couple the first chamber and the second chamber, and a second fluid path positioned to allow displaced gas to move out of the second chamber. The method can include moving displaced gas out of the second chamber via the second fluid path as a sterilant is moved into the second chamber via the first fluid path and/or as the liquid is moved into the second chamber via the first fluid path.Type: GrantFiled: September 17, 2014Date of Patent: January 10, 2017Inventors: Jeffrey D. Smith, Jeffrey C. Pederson, Sailaja Chandrapati, RuthAnn R. Duda
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Patent number: 9428786Abstract: A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.Type: GrantFiled: August 18, 2015Date of Patent: August 30, 2016Inventors: Jeffrey C. Pederson, Sailaja Chandrapati, Bryan S. Behun, Barry W. Robole, Leroy J. Longworth
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Patent number: D952137Type: GrantFiled: October 13, 2020Date of Patent: May 17, 2022Assignee: 3M INNOVATIVE PROPERTIES COMPANYInventors: Sailaja Chandrapati, Berenice Francoise Anne Marie Plessis, Elliott J. Zell, Adam J. Stanenas, Rocio Foncea, Samuel E. Beseres, Lloyd S. Vasilakes