Patent number: 11549119
Abstract: The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.
Type:
Grant
Filed:
April 6, 2017
Date of Patent:
January 10, 2023
Assignee:
ZUMUTOR BIOLOGICS, INC.
Inventors:
Sohang Chatterjee, Kavitha Iyer Rodrigues, Maloy Ghosh, Sunit Maity, Divya Unnikrishnan, Yogendra Manjunath Bangalore Muniraju, Sathyabalan Murugesan, Pavithra Mukunda, Bhargav Prasad, Veeresha Kamanagowda, Sanghamitra Bhattacharjee, Pravin Kumar Dakshinamurthy, Vivek Halan, Sankaranarayanan Srinivasan, Anuradha Hora, Bairavabalakumar Natarajan, Karthika Nair, Aswini Thanigaivel, Amol Maliwalave, Bharath Ravindra Shenoy, Sahana Bhima Rao, Subhra Prakash Chakrabarty, Ashvini Kumar Dubey, Amir Khan, Ankurina Sharma, Rashmi Sharma, Anurag Tiwari, Santosh Kumar, Shivani Patel, Nikitha M