Abstract: Synthetic biotextured surfaces and methods to produce the surface are disclosed. The surfaces mimic the topography of a surface of a naturally occurring sub-endothelial or sub-epithelial extracellular matrix. This is achieved by removing a cell layer from the naturally occurring extracellular matrix substrate, covering the extracellular matrix with a casting material, and allowing the casting material to harden. One then removes extracellular matrix from the casting material to uncover a negative core, and then uses the negative core to cast the replica material. By removing the negative core from the replica material one then creates the synthetic surface.